Amplification of a gene coding for human T-cell differentiation antigen

Using previously isolated mouse L-cell transferents for the human T-cell differentiation antigen Leu-2, we now report the first example of spontaneous gene amplification for membrane antigens. The Leu-2 (or T8) antigen is normally expressed on T lymphocytes that have cytotoxic or suppressor functions. Cells of a Leu-2 transfected clone were stained with fluorescein-tagged monoclonal anti-Leu-2, and the brightest 0.1-0.3% of cells were viably separated using a fluorescence activated cell sorter (FACS). After growth of these selected cells, sorting and regrowth was repeated six times, resulting in a population of cells that, compared with the starting population, stains 40 times brighter for Leu-2 and whose DNA transforms 20 times more efficiently for Leu-2. In addition, these cells have 10- to 50-fold amplified human DNA sequences and numerous double minute chromosome fragments, a common indicator of gene amplication in mouse cells.     

Size-dependent optical properties of Au nanorods

In the fast evolving field of nanoscience and nanotechnology,where size and shape are crucial in deciding the optoelectronic properties of nanomaterials,the understanding of size and shape dependent behavior is of direct relevance to device applications.Present study reports the synthesis of Au nanorods with well controlled aspect ratios,and the influence of the aspect ratio on the surface enhanced Raman scattering(SERS) activity using crystal violet(CV) as the probe molecule.The influence of pH and the concentrations of reducing agent and Ag ions in controlling the aspect ratio of gold nanorods are also investigated.The structural and optical properties of the synthesized samples have been characterized by transmission electron microscopy(TEM) and UV-visible absorption spectroscopy.The nonlinear optical(NLO) transmission of the Au nanorods investigated using the open aperture Z-scan technique revealed the absorption saturation followed by an optical limiting behavior,which may find potential applications in optoelectronic nanodevices.     

一种识别储层非均质性的双示踪剂方法

根据非均质多孔介质渗流与示踪剂迁移理论,提出一种识别储层非均质性的双示踪剂方法,通过构建考虑层间绕流的非均质单管模型,对双示踪剂方法进行试验验证。该方法利用两种扩散系数存在明显差别的示踪剂(离子型化学示踪剂溴化钾KBr和颗粒型荧光标记示踪剂荧光碳纳米颗粒Cdot)分别反映非均质多孔介质的总孔隙体积和非均质多孔介质内流动区的孔隙体积,识别储层非均质性和评价注入水的波及状况。结果表明:均质条件下KBr和Cdot的穿透曲线基本重合,而非均质条件下KBr和Cdot的穿透曲线产生分离,且KBr和Cdot在多孔介质中的流体置换率或改变率fv值相差较大;在一定的注入速率条件下,通过分析KBr和Cdot的产出规律及其fv值的变化规律,可识别储层非均质性和评价注入水的波及状况,验证了双示踪剂方法的可行性和有效性。     

义东油田义东断裂带成藏规律分析

 受义东断裂带控制,四扣洼陷北部发育了多种类型油气藏,且义东断裂带南北构造差异明显,造成成藏模式也存在较大差异。研究义东断裂带成藏条件及成藏规律,对该区的进一步油气勘探具有重要的意义。根据岩心、录井、钻井、地震等资料,对四扣洼陷义东断裂带成藏条件及成藏规律进行综合研究。结果表明,与义东断裂带相邻的四扣洼陷沙三、四段具有丰富的烃源岩条件、良好的储盖组合及封闭性,成藏条件十分优越。义东断裂带发育有多个岩性及构造－岩性油气藏。油藏具有沿断裂带分布及“浊积扇”式等特征。其中沙四段油气成藏特征为南部断阶带以礁灰岩储集层形成的岩性油藏,北部为碎屑岩储集层形成的构造－岩性油藏;沙三段发育小规模近岸水下扇、浊积扇构造－岩性油藏和浊积水道砂体岩性油藏;沙二段发育扇三角洲和砂质滩坝,常形成构造－岩性油藏。     

铝电解TiB2/C复合阴极用煤沥青的无机改性

采用无机粒子改性法在200℃下对煤沥青进行改性,分析无机粒子(Al2O3粒子或TiB2粒子)对改性煤沥青结构与性能以及改性沥青基TiB2/C复合阴极性能的影响。研究结果表明:改性煤沥青的结焦值和软化点比未改性煤沥青均有较大提高。无机粒子的添加进一步提高改性煤沥青的结焦能力,但降低改性煤沥青的软化点,与对比改性煤沥青相比,Al2O3粒子和TiB2粒子改性煤沥青的软化点分别降低4.5℃和2.0℃,结焦值分别提高4.85%和5.35%。经过1 000℃炭化,其残炭率相对于对比试样分别增加3.01%和5.87%。与对比复合阴极相比,Al2O3粒子和TiB2粒子改性煤沥青基复合阴极的密度分别增大0.45%和1.79%,开孔率分别降低5.08%和5.78%,电阻率从52.93μΩ·m分别降低到48.86μΩ·m和49.95μΩ·m,抗压强度从24.21 MPa分别增加到28.78 MPa和28.06 MPa,电解膨胀率分别降低0.10%和0.16%。无机改性粒子均匀地分布于粘结剂煤沥青炭中。     

Sub-laser-cycle electron pulses for probing molecular dynamics

Experience shows that the ability to make measurements in any new time regime opens new areas of science. Currently, experimental probes for the attosecond time regime (10(-18) 10(-15) s) are being established. The leading approach is the generation of attosecond optical pulses by ionizing atoms with intense laser pulses. This nonlinear process leads to the production of high harmonics during collisions between electrons and the ionized atoms. The underlying mechanism implies control of energetic electrons with attosecond precision. We propose that the electrons themselves can be exploited for ultrafast measurements. We use a 'molecular clock', based on a vibrational wave packet in H(2)(+) to show that distinct bunches of electrons appear during electron ion collisions with high current densities, and durations of about 1 femtosecond (10(-15) s). Furthermore, we use the molecular clock to study the dynamics of non-sequential double ionization.     

大跨度钢管混凝土拱桥肋缆索吊装施工技术

茅草街大桥为主跨368 m的自锚中承式钢管混凝土拱桥,在同类型桥型中跨度最大.主拱拱肋采用三跨缆索吊吊装施工,技术含量高,施工难度大,实践表明,茅草街大桥钢管拱肋缆索吊装施工较为成功,其经验值得为同类型桥梁施工借鉴.     

The p66shc adaptor protein controls oxidative stress response and life span in mammals

Gene mutations in invertebrates have been identified that extend life span and enhance resistance to environmental stresses such as ultraviolet light or reactive oxygen species. In mammals, the mechanisms that regulate stress response are poorly understood and no genes are known to increase individual life span. Here we report that targeted mutation of the mouse p66shc gene induces stress resistance and prolongs life span. p66shc is a splice variant of p52shc/p46shc (ref. 2), a cytoplasmic signal transducer involved in the transmission of mitogenic signals from activated receptors to Ras. We show that: (1) p66shc is serine phosphorylated upon treatment with hydrogen peroxide (H2O2) or irradiation with ultraviolet light; (2) ablation of p66shc enhances cellular resistance to apoptosis induced by H2O2 or ultraviolet light; (3) a serine-phosphorylation defective mutant of p66shc cannot restore the normal stress response in p66shc-/- cells; (4) the p53 and p21 stress response is impaired in p66shc-/- cells; (5) p66shc-/- mice have increased resistance to paraquat and a 30% increase in life span. We propose that p66shc is part of a signal transduction pathway that regulates stress apoptotic responses and life span in mammals.     

端到端推测网络路径上的数据包转发优先级

出于各种考虑,ISP会对不同类型的数据包设置不同的转发优先级. 这会造成不同网络应用出现网络性能上的差异,影响到网络测量结果的代表性,也会增加网络故障排查的难度. 通常情况下,ISP不公布此类信息. 为此提出了一种端到端测量方法,根据路径上不同类型包丢包率的差异来推断它们是否属于不同的转发优先级. 通过在PlanetLab上的测量实验,发现了位于某网络内部的一处优先级设置,结果得到了相关网管的确认.     

非线性空间有限元在钢筋混凝土分析中的应用

用非线性空间有限元法分析钢筋砼存所在的问题，包括材料的本构关系、破坏准则及砼开裂、压碎前后的模拟．在这些工作的基础上编制了三维非线性有限元分析程序．作为算例，分析了钢筋砼构件受扭矩等力系作用时的内力和位移．计算表明，理论与实验结果吻合良好．