农业网站的易访问性问题初探

阐述农业网站易访问性的现状,提出了增强网站易访问性的对策。     

ADO意义.NET在并发控制方面存在的问题及解决方案

针对ADO.NET在处理"丢失的修改"问题时,不支持"关键字和已修改字段"的并发控制问题提出了相应的解决办法;同时对ADO.NET架构本身带来的异常更新问题提供了解决思路.     

高职院校"软件蓝领"人才培养的探索与实践

结合教学实践,介绍了高职高专院校如何以管理信息系统课程的实践性教学为切入点培养"软件蓝领"人才的实践。     

Structural Changes of Water Molecules Upon the Reduction of Quinones in The Reaction Center from Rhodobactery Sphaeroides

1 Results The photosynthetic bacterial reaction center (RC) is a membrane protein complex.The RC is composed of three protein subunits and redox components such as bacteriochlorophylls, bacteriopheophytins,and quinones.The RC performs the photochemical electron transfer from the bacteriochlorophyll dimer through a series of electron donor and acceptor molecules to a secondary quinone,QB.QB accepts electrons from a primary quinone,QA,in two sequential electron transfer reactions.The second electron trans...     

浅谈随刊附盘的著录与管理

介绍了随刊附盘的种类及其验收与著录的方法,提出了随刊附盘管理和利用的措施。     

Transgenesis in fish

Gene transfer into fish embryo is being performed in several species (trout, salmon, carps, tilapia, medaka, goldfish, zebrafish, loach, catfish, etc.). In most cases, pronuclei are not visible and microinjection must be done into the cytoplasm of early embryos. Several million copies of the gene are generally injected. In medaka, transgenesis was attempted by injection of the foreign gene into the nucleus of oocyte. Several reports indicate that the injected DNA was rapidly replicated in the early phase of embryo development, regardless of the origin and the sequence of the foreign DNA. The survival of the injected embryos was reasonably good and a large number reached maturity. The proportion of transgenic animals ranged from 1 to 50% or more, according to species and to experimentators. The reasons for this discrepancy have not been elucidated. In all species, the transgenic animals were mosaic. The copy number of the foreign DNA was different in the various tissues of an animal and a proportion lower than 50% of F1 offsprings received the gene from their parents. This suggests that the foreign DNA was integrated into the fish genome at the two cells stage or later. An examination of the integrated DNA in different cell types of an animal revealed that integration occurred mainly during early development. The transgene was found essentially unrearranged in the fish genome of the founders and offsprings. The transgenes were therefore stably transmitted to progeny in a Mendelian fashion. Southern blot analysis revealed the presence of possible junction fragments and also of minor bands which may result from a rearrangement of the injected DNA. In all species, the integrated DNA appeared mainly as random end-to-end concatemers. In adult trout blood cells, a small proportion of the foreign DNA was maintained in the form of non-integrated concatemers, as judged by the existence of end fragments. The transgenes were generally only poorly expressed. The majority of the injected gene constructs contained essentially mammalian or higher vertebrates sequences. The comparison of the expression efficiency of these constructs in transfected fish and mammalian cells indicates that some of the mammalian DNA sequences are most efficiently understood by the fish cell machinery. Chloramphenicol acetyl transferase gene under the control of promoters from Rous sarcoma virus, and human cytomegalovirus, was expressed in several tissues of transgenic fish. Chicken delta-crystallin gene was expressed in several tissues of transgenic fish.(ABSTRACT TRUNCATED AT 400 WORDS)     

高中生高考前心理健康状况的调查与研究

在我国现阶段 ,高中生作为一个独立的社会群体 ,心理承受着巨大的压力 .学校在重视学习成绩的同时 ,往往忽视学生的生长发育规律 ;家长对孩子的关心也偏重于学习成绩 ,对生长发育期的孩子生理和心理的变化知之甚少 ,这些都加深了高中生的心理矛盾和心理冲突 ,使学生自身心理行为问题增多 ,患有心理疾病的学生人数增加 .这严重影响了学生身心的健康发展 ,影响素质教育目标的实现 .通过对齐齐哈尔 2所重点高中学生高考前心理健康状况调查以及对睡眠时间和参加体育锻炼的调查结果分析 ,探讨开展心理健康教育的必要性 ,研究实施心理健康教育的途径