浅谈随刊附盘的著录与管理

介绍了随刊附盘的种类及其验收与著录的方法,提出了随刊附盘管理和利用的措施。     

UNIX系统源语言调试器的分析

源语言调试器是程序员不可缺少的软件开发工具。本文从介绍UNIX系统程序调试的基本思想入手,较详细地分析了UNIX系统源语言调试器的实现,阐述了源语言调试器实现中的几个主要问题。     

Transgenesis in fish

Gene transfer into fish embryo is being performed in several species (trout, salmon, carps, tilapia, medaka, goldfish, zebrafish, loach, catfish, etc.). In most cases, pronuclei are not visible and microinjection must be done into the cytoplasm of early embryos. Several million copies of the gene are generally injected. In medaka, transgenesis was attempted by injection of the foreign gene into the nucleus of oocyte. Several reports indicate that the injected DNA was rapidly replicated in the early phase of embryo development, regardless of the origin and the sequence of the foreign DNA. The survival of the injected embryos was reasonably good and a large number reached maturity. The proportion of transgenic animals ranged from 1 to 50% or more, according to species and to experimentators. The reasons for this discrepancy have not been elucidated. In all species, the transgenic animals were mosaic. The copy number of the foreign DNA was different in the various tissues of an animal and a proportion lower than 50% of F1 offsprings received the gene from their parents. This suggests that the foreign DNA was integrated into the fish genome at the two cells stage or later. An examination of the integrated DNA in different cell types of an animal revealed that integration occurred mainly during early development. The transgene was found essentially unrearranged in the fish genome of the founders and offsprings. The transgenes were therefore stably transmitted to progeny in a Mendelian fashion. Southern blot analysis revealed the presence of possible junction fragments and also of minor bands which may result from a rearrangement of the injected DNA. In all species, the integrated DNA appeared mainly as random end-to-end concatemers. In adult trout blood cells, a small proportion of the foreign DNA was maintained in the form of non-integrated concatemers, as judged by the existence of end fragments. The transgenes were generally only poorly expressed. The majority of the injected gene constructs contained essentially mammalian or higher vertebrates sequences. The comparison of the expression efficiency of these constructs in transfected fish and mammalian cells indicates that some of the mammalian DNA sequences are most efficiently understood by the fish cell machinery. Chloramphenicol acetyl transferase gene under the control of promoters from Rous sarcoma virus, and human cytomegalovirus, was expressed in several tissues of transgenic fish. Chicken delta-crystallin gene was expressed in several tissues of transgenic fish.(ABSTRACT TRUNCATED AT 400 WORDS)     

论信托法的理论及实践

信托制度最根本的特点是“所有权和利益相分离”。信托财产的独立性使商事信托成为其他法律制度如代理或合同所不能替代的法律制度。从效率的角度出发,证明信托制度的科学性和实用性及其法理价值,并对构建独持养老金制度和集合投资信托制度的商事设计进行探讨。     

城市规划辅助审批系统中的相似度研究

城市规划的审批是一件难于量化的工作，它受政治经济等多方面因素影响，是城市规划领域关注的一大焦点．本文尝试将CBR技术用于城市规划辅助审批，让政府的审批依据达到历史与现实的统一．实现CBR的一个关键是相似度的设计，本文结合城市规划辅助审批领域的特点，设计了可行的方案，并应用于实际的办公系统中．     

激发数学学习兴趣的策略

针对多数学生对学习数学有枯燥、乏味的感觉，阐述了必须在教学中采用生动的富有感染力的教学方法，以激发学生对数学学习的浓厚兴趣，使学生在提出问题和解决问题中养成善于思考，勇于探索的良好品质。     

漫谈音乐院校钢琴副科教学的艺术

钢琴副科课是我国音乐院校针对非钢琴专业的学生开设的一门钢琴课程.本文以如何上好钢琴副科课为探讨的主要目的,从基础和提高视谱能力这两个角度来展开讨论,力图使学生学习更富有热情和效率,对知识的掌握更为主动和全面.     

新疆北塔山天然草地营养类型的初步研究

通过对新疆北塔山天然草地实地考察，采集混合草群样品，经化学分析，按照全国统一标准和方法，对北塔山天然草地进行了营养类型划分，阐述了各营养类型草地自然、经济特征，并对各营养类型草地的营养特点、饲用价值和适宜放牧家畜配置等进行了分析评价。     

分子间作用力对制备含有机废弃之水泥浆体影响研究

研究以水泥处理固体有机物时，有机物对水泥水化的影响。以膜流模式说明水份在水泥浆体内传输的现象。水膜存在半干之水泥浆体，其厚度小于100nm，膜流由离分压所驱动，而离分压来自分子间作用力。膜拟结果发现，水泥浆体内局部位置可用以进行水化之水量为Q=-Aslv／6vπ。当有机物添加在水泥浆体内时，会影响Hamaker常数，Aslv因此可能使膜流不稳定或完全抑制膜流，因而使水泥浆体内局部缺水，影响水化进行，造成成品强度降低，影响品质。