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71.
1 Results The photosynthetic bacterial reaction center (RC) is a membrane protein complex.The RC is composed of three protein subunits and redox components such as bacteriochlorophylls, bacteriopheophytins,and quinones.The RC performs the photochemical electron transfer from the bacteriochlorophyll dimer through a series of electron donor and acceptor molecules to a secondary quinone,QB.QB accepts electrons from a primary quinone,QA,in two sequential electron transfer reactions.The second electron trans... 相似文献
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73.
李敏 《新乡学院学报(自然科学版)》2008,(2)
在深入分析本科生研究能力存在的问题以及本科生研究能力内涵的基础上,给出相应的本科生研究能力阶段性培养方案,试图通过多种训练方法的有机结合,解决本科生研究能力培养的问题。 相似文献
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荣敏 《徐州师范大学学报(自然科学版)》1996,(2)
对梅向明等编《高等几何》关于线束中四直线交比的内容作重新编排,既保证其内容的完整性,又弥补原书理论上的不足. 相似文献
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Transgenesis in fish 总被引:1,自引:0,他引:1
Gene transfer into fish embryo is being performed in several species (trout, salmon, carps, tilapia, medaka, goldfish, zebrafish, loach, catfish, etc.). In most cases, pronuclei are not visible and microinjection must be done into the cytoplasm of early embryos. Several million copies of the gene are generally injected. In medaka, transgenesis was attempted by injection of the foreign gene into the nucleus of oocyte. Several reports indicate that the injected DNA was rapidly replicated in the early phase of embryo development, regardless of the origin and the sequence of the foreign DNA. The survival of the injected embryos was reasonably good and a large number reached maturity. The proportion of transgenic animals ranged from 1 to 50% or more, according to species and to experimentators. The reasons for this discrepancy have not been elucidated. In all species, the transgenic animals were mosaic. The copy number of the foreign DNA was different in the various tissues of an animal and a proportion lower than 50% of F1 offsprings received the gene from their parents. This suggests that the foreign DNA was integrated into the fish genome at the two cells stage or later. An examination of the integrated DNA in different cell types of an animal revealed that integration occurred mainly during early development. The transgene was found essentially unrearranged in the fish genome of the founders and offsprings. The transgenes were therefore stably transmitted to progeny in a Mendelian fashion. Southern blot analysis revealed the presence of possible junction fragments and also of minor bands which may result from a rearrangement of the injected DNA. In all species, the integrated DNA appeared mainly as random end-to-end concatemers. In adult trout blood cells, a small proportion of the foreign DNA was maintained in the form of non-integrated concatemers, as judged by the existence of end fragments. The transgenes were generally only poorly expressed. The majority of the injected gene constructs contained essentially mammalian or higher vertebrates sequences. The comparison of the expression efficiency of these constructs in transfected fish and mammalian cells indicates that some of the mammalian DNA sequences are most efficiently understood by the fish cell machinery. Chloramphenicol acetyl transferase gene under the control of promoters from Rous sarcoma virus, and human cytomegalovirus, was expressed in several tissues of transgenic fish. Chicken delta-crystallin gene was expressed in several tissues of transgenic fish.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
78.
闵洁 《东华大学学报(自然科学版)》2002,28(1):97-100,104
对复合变性玉米淀粉印花糊料的增稠性,稳定性,印花特性及流变性等方面作了系统研究,并认为此糊料可以全部或部分代替海藻酸钠。 相似文献
79.
钢琴副科课是我国音乐院校针对非钢琴专业的学生开设的一门钢琴课程.本文以如何上好钢琴副科课为探讨的主要目的,从基础和提高视谱能力这两个角度来展开讨论,力图使学生学习更富有热情和效率,对知识的掌握更为主动和全面. 相似文献
80.
本文提出了大截面消声器的设计方法和步聚,并对大型消声器的制造技术及其应用效果进行了探讨. 相似文献