Heritable germline epimutation of MSH2 in a family with hereditary nonpolyposis colorectal cancer

Epimutations in the germline, such as methylation of the MLH1 gene, may contribute to hereditary cancer syndrome in human, but their transmission to offspring has never been documented. Here we report a family with inheritance, in three successive generations, of germline allele-specific and mosaic hypermethylation of the MSH2 gene, without evidence of DNA mismatch repair gene mutation. Three siblings carrying the germline methylation developed early-onset colorectal or endometrial cancers, all with microsatellite instability and MSH2 protein loss. Clonal bisulfite sequencing and pyrosequencing showed different methylation levels in different somatic tissues, with the highest level recorded in rectal mucosa and colon cancer tissue, and the lowest in blood leukocytes. This mosaic state of germline methylation with different tissue distribution could act as the first hit and provide a mechanism for genetic disease inheritance that may deviate from the mendelian pattern and be overlooked in conventional leukocyte-based genetic diagnosis strategy.     

英语转移否定句的理解与翻译

在翻译中,英语的否定结构是一个常见的语言现象.由于英汉两种语言在表达方法上存在很大差异,尤其在表达否定概念上,英语在用词、语法和逻辑等方面与汉语都有很大不同.如何将英语否定句的确切含义准确地用汉语表达出来,是一个较为复杂的事.本文就英语转移否定句的理解与翻译从3个方面进行了探讨.     

切削用量对刀具耐用度影响的探讨

简述了切削用量对刀具耐用度的影响程度,叙述了在零件加工过程中合理的选取切削用量和合适的刀具耐用度,对于提高机械加工的效率有很大的作用.     

Effect of protein molecules on the photoluminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots

The fact that the photoluminescence properties of quantum dots are always strongly influenced by the environment limits the scope of further progress in the field of QD’ bio-applications. In this paper, the effects of immunoglobulin G (IgG) on the photo-luminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots coated with amphiphilic poly (acrylic acid) (PAA) are studied. Photoluminescence (PL) spectra, UV-vis spectra and excited state lifetime measurements are used to characterize the influence of different protein molecules, such as IgG (goat anti-human IgG, rabbit anti-human IgG, human IgG, and goat anti-human IgG-human IgG conjugates), avidin and bovine serum albumin (BSA) on the PL properties of QDs. The PL intensity and stability of CdSe/ZnS are largely enhanced compared to that of pure CdSe/ZnS QDs when the IgG molecules are added into the QD solution. The PL intensity increases with increasing the IgG concentration, but there appears no influence on the PL peak and a full width at half maximum (FWHM). The PL evolution of QDs as a function of different protein molecules depends on the structure of protein molecules, which is used as a sensor to recognize human IgG. It is inferred that the interaction between PAA coating layer and IgG molecules results in the enhancement of PL intensity. The study of the effect of pH and ion strength on optical properties of QD-IgG mixed solution, compared with the pure QD solution, suggests that pH value and ion strength do not destroy the interaction between the PAA coating layer and IgG. Excited state lifetime analysis indicates that the PL enhancement comes from the passivation of surface of the QDs with the PAA coating layer. IgG molecules have no effects on the properties of the biological system but can increase the stability and PL intensity of CdSe/ZnS QDs, which will enlarge the application of QDs in biomedicine and other fields.     

一类商品价格供需系统的稳定性分析

运用动力系统稳定性理论,建立了一类具有时滞脉冲的商品价格供需系统,讨论了一类商品价格供需系统的稳定性问题.给出了系统在时滞脉冲下零解渐近稳定的条件,分析系统参数、脉冲和时滞量之间的相对关系对系统的影响,发现在一定条件下商品市场的价格调节机制是可行的,能够保证市场商品价格的稳定,但价格这种调节机制是有限的,一旦超出这个范围,就会出现市场商品价格的不稳定.最后进行数值仿真,验证了时滞脉冲稳定的有效性,并给出了经济学解释.     

互联网时代的“数字化校园”建设与高校管理工作的思考

随着互联网的飞速发展,教育界掀起了一股数字化校园建设热潮,众多的学校纷纷投入到其中,架构在网络环境上的＂数字化校园＂成为学校信息化建设的重点。搞好校园网络建设,构建现代化的教育环境,成为教育现代化的重要内容,也是形成现代教育办学体系的重要组成部分。我们的数字化校园建设是要以网络技术、多媒体技术等现代化信息技术为手段、以提高教育教学工作效率为目的,实现资源的有效整合和优化,从根本上转变教育教学和管理模式与方法。因此,我们要从根本上转变思想,建立与完善现代化管理意识的校园网络;同时,加强师资队伍建设,培养一支素养高、勇于创新、善于运用现代化技术的教师队伍。     

无界绝对正则序列强律的收敛速度

本文进一步讨论了无界绝对正则序列(未必平稳)的Marcinkiewicz-Zygmund强律的收敛速度。在对随机变量的矩与相对系数给出一定的限制后,得到了与[2]中主要结果相应的结论。     

吖啶橙荧光染色法在遗传毒理学中的应用

本文用吖啶橙(Acridine orange)荧光染色法进行了遗传毒理的研究,并取得了较好的效果。实验内容包括:(1)中国仓鼠体外培养细胞的姐妹染色单体交换试验;(2)中国仓鼠活体中姐妹染色单体交换试验;(3)小鼠骨髓嗜多染红细胞微核试验;(4)孕鼠骨髓与胎鼠肝血微核试验。与Giemsa染色法相比,吖啶橙荧光染色法有染色特异性强、色泽鲜艳、重复性强、实验结果可靠等优点.     

墨子的数学思想

本文将墨子的数学思想作为一个整体来研究,揭示了它的起源及本质,探讨了数学与政治、文化及哲学的关系.文章着重阐述墨子是怎样把墨家特有的形式逻辑——辩学,用于数学研究的,并认为在墨子的数学理论中,整体观是基础,抽象性是主要特征,而关于无限的论述则代表着墨子数学思想的最高水平.文中还探讨了墨家衰亡的社会原因,提出一些值得今天借鉴的历史教训.