Relationships between insolation and rattlesnake hibernacula

We examined the relationship between insolation, climate, and hibernacula of black-tailed ( Crotalus molossus ), Great Basin ( Crotalus lutosus ), and western diamondback ( Crotalus atrox ) rattlesnakes at 4 sites in Arizona, Nevada, and Utah. Hibernacula were located through a combination of visual searches and radio telemetry from 1995 to 2003. We used global information systems to calculate insolation and compared hibernaculum insolation values with random points representing available insolation of the surrounding habitat. Insolation reflects soil temperatures, and we predicted that hibernacula in cool climates, at high elevations, and at high latitudes would have higher insolation relative to their surroundings, while hibernacula in warmer climates would not differ from their surroundings in insolation. Coolest temperatures, highest elevations, and highest latitudes occurred on the C. lutosus and C. molossus sites, where hibernaculum insolation was higher than surrounding insolation. Temperatures were intermediate on the high-elevation C. atrox site, where hibernaculum insolation did not differ from random-point insolation. Temperatures were highest on the low-elevation C. atrox site, where hibernaculum insolation was unexpectedly lower than random-point insolation. Our observations suggest that rattlesnakes in cool climates utilize hibernacula with insolation values higher than those of their surroundings. Rattlesnakes in warm climates utilize hibernacula with insolation values lower than or similar to those of their surroundings.     

Complex shapes self-assembled from single-stranded DNA tiles

Programmed self-assembly of strands of nucleic acid has proved highly effective for creating a wide range of structures with desired shapes. A particularly successful implementation is DNA origami, in which a long scaffold strand is folded by hundreds of short auxiliary strands into a complex shape. Modular strategies are in principle simpler and more versatile and have been used to assemble DNA or RNA tiles into periodic and algorithmic two-dimensional lattices, extended ribbons and tubes, three-dimensional crystals, polyhedra and simple finite two-dimensional shapes. But creating finite yet complex shapes from a large number of uniquely addressable tiles remains challenging. Here we solve this problem with the simplest tile form, a 'single-stranded tile' (SST) that consists of a 42-base strand of DNA composed entirely of concatenated sticky ends and that binds to four local neighbours during self-assembly. Although ribbons and tubes with controlled circumferences have been created using the SST approach, we extend it to assemble complex two-dimensional shapes and tubes from hundreds (in some cases more than one thousand) distinct tiles. Our main design feature is a self-assembled rectangle that serves as a molecular canvas, with each of its constituent SST strands--folded into a 3 nm-by-7 nm tile and attached to four neighbouring tiles--acting as a pixel. A desired shape, drawn on the canvas, is then produced by one-pot annealing of all those strands that correspond to pixels covered by the target shape; the remaining strands are excluded. We implement the strategy with a master strand collection that corresponds to a 310-pixel canvas, and then use appropriate strand subsets to construct 107 distinct and complex two-dimensional shapes, thereby establishing SST assembly as a simple, modular and robust framework for constructing nanostructures with prescribed shapes from short synthetic DNA strands.     

Decoherence of quantum superpositions through coupling to engineered reservoirs

The theory of quantum mechanics applies to closed systems. In such ideal situations, a single atom can, for example, exist simultaneously in a superposition of two different spatial locations. In contrast, real systems always interact with their environment, with the consequence that macroscopic quantum superpositions (as illustrated by the 'Schrodinger's cat' thought-experiment) are not observed. Moreover, macroscopic superpositions decay so quickly that even the dynamics of decoherence cannot be observed. However, mesoscopic systems offer the possibility of observing the decoherence of such quantum superpositions. Here we present measurements of the decoherence of superposed motional states of a single trapped atom. Decoherence is induced by coupling the atom to engineered reservoirs, in which the coupling and state of the environment are controllable. We perform three experiments, finding that the decoherence rate scales with the square of a quantity describing the amplitude of the superposition state.     

Genome sequence of Silicibacter pomeroyi reveals adaptations to the marine environment

Since the recognition of prokaryotes as essential components of the oceanic food web, bacterioplankton have been acknowledged as catalysts of most major biogeochemical processes in the sea. Studying heterotrophic bacterioplankton has been challenging, however, as most major clades have never been cultured or have only been grown to low densities in sea water. Here we describe the genome sequence of Silicibacter pomeroyi, a member of the marine Roseobacter clade (Fig. 1), the relatives of which comprise approximately 10-20% of coastal and oceanic mixed-layer bacterioplankton. This first genome sequence from any major heterotrophic clade consists of a chromosome (4,109,442 base pairs) and megaplasmid (491,611 base pairs). Genome analysis indicates that this organism relies upon a lithoheterotrophic strategy that uses inorganic compounds (carbon monoxide and sulphide) to supplement heterotrophy. Silicibacter pomeroyi also has genes advantageous for associations with plankton and suspended particles, including genes for uptake of algal-derived compounds, use of metabolites from reducing microzones, rapid growth and cell-density-dependent regulation. This bacterium has a physiology distinct from that of marine oligotrophs, adding a new strategy to the recognized repertoire for coping with a nutrient-poor ocean.     

Karyopherin-mediated import of integral inner nuclear membrane proteins

Targeting of newly synthesized integral membrane proteins to the appropriate cellular compartment is specified by discrete sequence elements, many of which have been well characterized. An understanding of the signals required to direct integral membrane proteins to the inner nuclear membrane (INM) remains a notable exception. Here we show that integral INM proteins possess basic sequence motifs that resemble 'classical' nuclear localization signals. These sequences can mediate direct binding to karyopherin-alpha and are essential for the passage of integral membrane proteins to the INM. Furthermore, karyopherin-alpha, karyopherin-beta1 and the Ran GTPase cycle are required for INM targeting, underscoring parallels between mechanisms governing the targeting of integral INM proteins and soluble nuclear transport. We also provide evidence that specific nuclear pore complex proteins contribute to this process, suggesting a role for signal-mediated alterations in the nuclear pore complex to allow for passage of INM proteins along the pore membrane.