全文获取类型
收费全文 | 276篇 |
免费 | 0篇 |
国内免费 | 3篇 |
专业分类
系统科学 | 3篇 |
教育与普及 | 1篇 |
理论与方法论 | 3篇 |
现状及发展 | 58篇 |
研究方法 | 32篇 |
综合类 | 172篇 |
自然研究 | 10篇 |
出版年
2018年 | 3篇 |
2017年 | 2篇 |
2016年 | 1篇 |
2015年 | 1篇 |
2014年 | 2篇 |
2013年 | 1篇 |
2012年 | 23篇 |
2011年 | 24篇 |
2010年 | 4篇 |
2009年 | 1篇 |
2008年 | 18篇 |
2007年 | 12篇 |
2006年 | 22篇 |
2005年 | 16篇 |
2004年 | 14篇 |
2003年 | 10篇 |
2002年 | 8篇 |
2001年 | 9篇 |
2000年 | 7篇 |
1999年 | 5篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 6篇 |
1989年 | 2篇 |
1988年 | 5篇 |
1986年 | 1篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 4篇 |
1979年 | 1篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1975年 | 4篇 |
1974年 | 2篇 |
1973年 | 1篇 |
1972年 | 2篇 |
1971年 | 7篇 |
1970年 | 8篇 |
1969年 | 6篇 |
1968年 | 3篇 |
1967年 | 3篇 |
1966年 | 8篇 |
1965年 | 3篇 |
1963年 | 1篇 |
1958年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有279条查询结果,搜索用时 171 毫秒
11.
Davila S Furu L Gharavi AG Tian X Onoe T Qian Q Li A Cai Y Kamath PS King BF Azurmendi PJ Tahvanainen P Kääriäinen H Höckerstedt K Devuyst O Pirson Y Martin RS Lifton RP Tahvanainen E Torres VE Somlo S 《Nature genetics》2004,36(6):575-577
Mutations in PRKCSH, encoding the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER), cause autosomal dominant polycystic liver disease. We found that mutations in SEC63, encoding a component of the protein translocation machinery in the ER, also cause this disease. These findings are suggestive of a role for cotranslational protein-processing pathways in maintaining epithelial luminal structure and implicate noncilial ER proteins in human polycystic disease. 相似文献
12.
Kawai J Shinagawa A Shibata K Yoshino M Itoh M Ishii Y Arakawa T Hara A Fukunishi Y Konno H Adachi J Fukuda S Aizawa K Izawa M Nishi K Kiyosawa H Kondo S Yamanaka I Saito T Okazaki Y Gojobori T Bono H Kasukawa T Saito R Kadota K Matsuda H Ashburner M Batalov S Casavant T Fleischmann W Gaasterland T Gissi C King B Kochiwa H Kuehl P Lewis S Matsuo Y Nikaido I Pesole G Quackenbush J Schriml LM Staubli F Suzuki R Tomita M Wagner L Washio T Sakai K Okido T Furuno M Aono H Baldarelli R Barsh G 《Nature》2001,409(6821):685-690
The RIKEN Mouse Gene Encyclopaedia Project, a systematic approach to determining the full coding potential of the mouse genome, involves collection and sequencing of full-length complementary DNAs and physical mapping of the corresponding genes to the mouse genome. We organized an international functional annotation meeting (FANTOM) to annotate the first 21,076 cDNAs to be analysed in this project. Here we describe the first RIKEN clone collection, which is one of the largest described for any organism. Analysis of these cDNAs extends known gene families and identifies new ones. 相似文献
13.
In the Northern Hemisphere, ozone levels in the troposphere have increased by 35 per cent over the past century, with detrimental impacts on forest and agricultural productivity, even when forest productivity has been stimulated by increased carbon dioxide levels. In addition to reducing productivity, increased tropospheric ozone levels could alter terrestrial carbon cycling by lowering the quantity and quality of carbon inputs to soils. However, the influence of elevated ozone levels on soil carbon formation and decomposition are unknown. Here we examine the effects of elevated ozone levels on the formation rates of total and decay-resistant acid-insoluble soil carbon under conditions of elevated carbon dioxide levels in experimental aspen (Populus tremuloides) stands and mixed aspen-birch (Betula papyrifera) stands. With ambient concentrations of ozone and carbon dioxide both raised by 50 per cent, we find that the formation rates of total and acid-insoluble soil carbon are reduced by 50 per cent relative to the amounts entering the soil when the forests were exposed to increased carbon dioxide alone. Our results suggest that, in a world with elevated atmospheric carbon dioxide concentrations, global-scale reductions in plant productivity due to elevated ozone levels will also lower soil carbon formation rates significantly. 相似文献
14.
Seismic hazard in the Marmara Sea region following the 17 August 1999 Izmit earthquake 总被引:3,自引:0,他引:3
Hubert-Ferrari A Barka A Jacques E Nalbant SS Meyer B Armijo R Tapponnier P King GC 《Nature》2000,404(6775):269-273
On 17 August 1999, a destructive magnitude 7.4 earthquake occurred 100 km east of Istanbul, near the city of Izmit, on the North Anatolian fault. This 1,600-km-long plate boundary slips at an average rate of 2-3 cm yr(-1), and historically has been the site of many devastating earthquakes. This century alone it has ruptured over 900 km of its length. Models of earthquake-induced stress change combined with active fault maps had been used to forecast that the epicentral area of the 1999 Izmit event was indeed a likely location for the occurrence of a large earthquake. Here we show that the 1999 event itself significantly modifies the stress distribution resulting from previous fault interactions. Our new stress models take into account all events in the region with magnitudes greater than 6 having occurred since 1700 as well as secular interseismic stress change, constrained by GPS data. These models provide a consistent picture of the long term spatio-temporal behaviour of the North Anatolian fault and indicate that two events of magnitude equal to, or greater than, the Izmit earthquake are likely to occur within the next decades beneath the Marmara Sea, south of Istanbul. 相似文献
15.
下肢损伤是行人与车辆碰撞事故中的主要损伤形式之一,建立具有较高生物逼真度的行人下肢有限元模型,可以为下肢保护提供有效的研究手段.在下肢长骨(股骨、胫骨、腓骨)、韧带(ACL,PCL,MCL,LCL)及膝关节得到全面验证的基础上,建立了包含骨骼、韧带、肌肉及皮肤等详细解剖学结构的行人下肢有限元模型,并定义了各组织间的接触.利用行人下肢弯曲的生物力学实验,对下肢有限元模型进行了整体弯曲验证.结果显示,下肢有限元模型的损伤形式、损伤发生时刻、膝关节弯矩和弯曲角度均与实验结果吻合较好,能够较真实地反映行人下肢的损伤和生物力学响应,具有较好的生物逼真度. 相似文献
16.
17.
A D Hope G A King 《厦门大学学报(自然科学版)》2002,(Z1)
One of the most important features of the modern ma ch ining system in an “unmanned“ factory is to change tools that have been subjec ted to wear and damage. An integrated tool condition monitoring system composed of multi-sensors, signal processing devices and intelligent decision making pla ns is a necessary requirement for automatic manufacturing processes. An intellig ent tool wear monitoring system will be introduced in this paper. The system is equipped with power consumption, vibration, AE and cutt... 相似文献
18.
Progesterone inhibits the 21-hydroxylation of 17alpha-hydroxyprogesterone by human adrenal cortex microsomes. The possible light this finding may shed on the genetic condition, the 'adrenogenital syndrome' is discussed. Km and Vmax data for the above hydroxylation reaction are given. 相似文献
19.
Computed redox potentials and the design of bioreductive agents 总被引:1,自引:0,他引:1
Anti-cancer agents that have been made selective for tumour cells by exploiting the known differences in the availability of oxygen between normal and transformed cells are a promising development in cancer chemotherapy. We have recently suggested a new type of bioreductive activity which would depend on a two-electron reduction. For rational design of such compounds, it is essential to be able to predict the redox potentials and the chemical modifications needed to produce the optimum redox value. Calculating redox potentials is a daunting task for the theoretician, however, as the effect of water solvation is clearly of major significance. Recent successful calculations of differences in the free energies of biologically important molecules in aqueous solution using the free-energy perturbation method prompted us to apply the technique to the computation of two-electron redox potentials. The results are accurate to within 20 mV, suggesting that we should be able to manipulate redox potentials by successfully predicting structures with the appropriate value. 相似文献
20.
Catalytic head assembling protein in virus morphogenesis 总被引:25,自引:0,他引:25