Monitoring p21 mRNA expression in living cell based on molecular beacon fluorescence increasing rate

Studying the expression level of mRNA in living cells will offer tremendous opportunities for advancement in cell biology research, disease diagnostics, and drug discovery. In this paper, a molecular beacon （MB） specific for the important tumor suppressor gene p21 has been designed and synthesized. The fluorescence signal was detected in real-time after the MB entered the cytoplasm of nasopharyngeal carcinoma cells. After injecting the p21MB into nasopharyngeal carcinoma cell and p33-transfected nasopharyngeal carcinoma cell, the consistent increase of fluorescent signal intensity was detected in both cell lines, and maximum fluorescence intensity achieved in about 15 min. In about 4 min following microinjection, the fluorescence increasing rate was significantly different between these two cell lines, which indicate the different p21 mRNA expression levels. The results obtained in the real-time detection were also validated by RT-PCR. Analysis of the initial fluorescence increasing rate can efficiently reduce the side effect of enzyme and improve the accuracy in living cell mRNA detection.     

基于激光散透比的牛乳脂肪测试技术研究

依据光散射原理,建立了利用散透比测量牛乳脂肪质量含量的理论模型,提出了牛乳脂肪质量含量的快速高准确度测量方法。实验结果表明,本方法能准确、快速的测量牛乳脂肪质量含量。     

铝（Ⅲ）在乳状液膜体系中的迁移行为

研究了铝(Ⅲ)在乳状液膜分离体系中的迁移行为.确立了最佳迁移条件: 制乳搅拌速度2 400 r/min,制乳时间10 min,乳液与外相溶液混合时间15 min,乳水比0.4,磷酸三丁酯(TBP)浓度4.5 %,span80和液体石蜡浓度均为7.9 %,内相和外相HCl浓度分别为1.0 mol/L和3.0 mol/L.在此条件下,铝(Ⅲ)的迁移率达90 %以上.     

B3G系统中MAC和PHY接口设计与实现

研究了如何实现超三代移动通信时分双工(B3G TDD)系统中媒质接人控制层(MAC)与物理层(PHY)多通道RocketIO Gbit数据接口.基于模块化思想设计出一种按比特映射方式调整MAC发送数据包格式的透明发送机制;利用各通道轮询方法设计了一种多通道联合自适应数据接收机制,它的片内存储器资源占用率仅为传统FPGA设计的25%.基于该接口模式,仅修改FPGA内几个寄存器值即町实现整个系统的升级,而自适应接收机制使得基站和移动台可以使用同一FPGA代码,即实现了设计的通用性.所有寄存器均由协议处理器通过外部设备互联总线配置(PCI),因此FPGA配置文件只需要生成一次,可节省大量用于产生不同配置文件所需的FPGA布局布线的时间.本接口设计方法在未来移动通信系统中具有广泛的应用价值.     

超宽带无线通信系统接入控制流程设计

针对单信道不能解决并发传输的问题,在超宽带系统的接入控制中采用多信道方式,根据信道最大利用率的原则决定哪些分组可先传送.对原有的接入控制设计进行了适用于超宽系统特点的改进,通过退避一段随机时间再来监听信道.在半双工方式下采用单收发器方式进行系统设计,每个节点依据服务质量需求选择确定的分组,分组信息广播到所有节点,所有信息在信号产生阶段收集以减小系统额外开销.仿真结果表明,改进的设计在业务量高时传输性能更好.     

从构建和谐社会角度看老年休闲的发展

本文在分析我国老年人口状况和老龄化状况、老年休闲的类型、特点及存在问题的基础上,阐述了和谐社会构建进程中发展老年休闲的重要意义,并提出了发展老年休闲的相关对策,旨在为科学应对人口老龄化,促进和谐社会建设提供理论参考。     

Efficient statistical analysis method of power/ground(P/G) network

In this paper, we propose an incremental statistical analysis method with complexity reduction as a pre-process for on-chip power/ground (P/G) networks. The new method exploits locality of P/G network analyses and aims at P/G networks with a large number of strongly connected subcircuits(called strong connects) such as trees and chains. The method consists of three steps. First it compresses P/G circuits by removing strong connects. As a result, current variations (CV) of nodes in strong connects are transferred to some remaining nodes. Then based on the locality of power grid voltage responses to its current inputs, it efficiently calculates the correlative resistor (CR) matrix in a local way to directly compute the voltage variations by using small parts of the remaining circuit. Last it statistically recovers voltage variations of the suppressed nodes inside strong connects. This new method for statistically compressing and expanding strong connects in terms of current or voltage variations in a closed form is very efficient owning to its property of incremental analysis. Experimental results demonstrate that the method can efficiently compute low-bounds of voltage variations for P/G networks and it has two or three orders of magnitudes speedup over the traditional Monte-Carlo-based simulation method, with only 2.0% accuracy loss.     

多媒体网络化大学英语课堂教学提问艺术探究

多媒体网络化教学的进一步普及,要求英语教师的课堂提问也不断改革,并且更加艺术化.英语课堂提问艺术要注重激发学生学习兴趣,培养学生分析问题、解决问题的能力,充分发挥学生的主体作用.     

Extending TAM for Online Learning Systems: An Intrinsic Motivation Perspective

To get a better understanding of user behavior towards online learning systems, the technology acceptance model （TAM） was extended to include an intrinsic motivational factor. An online survey posted on a campus BBS was conducted to collect research data with a total of 121 usable responses. The results support the motivational model and show that the explained variance of online learning system use behavior is 71.3% higher than that of the original TAM explanation.     

无机盐水解法制备介孔NiAl2O4纳米粉简

介孔NiAl2 O4材料是常用的电极材料和催化材料,目前常用溶胶-凝胶法制备。本文在不添加任何模板剂和有机溶剂的情况下,通过摩尔比为1！2的硝酸镍和硝酸铝水溶液与碳酸铵水溶液水解的方法制备NiAl2 O4介孔材料。利用热重-差热分析、X射线衍射、扫描电镜观察、透射电镜观察及N2吸附-脱附等测试手段表征。通过800℃焙烧,制备了NiAl2 O4介孔材料,晶粒大小约30 nm,比表面积为105．3 m2/g,平均孔径为7．2 nm,孔容为0．19 cm3/g,孔径分布比较狭窄、规整。