Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis

To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme (hLYZ) cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400 μg of the p215C3LYZ vector, over 2.0 μg/ml of rhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhLYZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hLYZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis.     

略论网络时代高校思想政治工作

网络技术的发展给高校思想政治工作带来了难得的机遇，同时也面临严峻的挑战。面对机遇和挑战，高校思想政治工作者必须积极应对，趋利避害。充分发挥网络的积极作用，加强和改进高校思想政治工作。     

稀土金属有机氢化物的合成及其引发Claisen 重排反应的研究

用(C9H7)2LnCl@2THF与过量氢化钠在THF溶液中反应,得到相应的稀土有机氢化物[(C9H7)2LnH]2@4THF@NaCl,用制得的氢化物与不饱和羰基化合物苯乙酸烯丙酯反应,发生Claisen重排,同时选择性还原羰基.     

基于模糊神经网络方法的新构造控水专家系统

在对大量野外地质资料统计和综合分析基础上，依据地质专家在新构造领域的理论知识和成功经验，运用模糊数学理论和神经网络理论相结合的方法，提出一种用线性单元并联实现非线性划分的神经元网络模型，并研制出相应具有较强推理能力和学习功能、广泛用于非定性系统数据处理的专家系统，该系统经实例验证具有较强的适用性。     

微乳液介质中5''-硝基水杨基荧光酮光度法测定微量锰

研究了在混合微乳液介质中 ,Mn(Ⅱ) - 5’ -硝基水杨基荧光酮 (5’ -NSF)的显色反应 ,在pH =9.5～ 10 .5的硼砂缓冲体系中 ,Mn(Ⅱ)与 5’ -NSF形成 1∶2的紫红色配合物 ,ε548=1.4 4× 10 5L·mol- 1 ·mL- 1 ,锰质量浓度在 0～ 0 .2 8mg·L- 1 范围内服从比尔定律。该方法用于岩石矿物及水中微量锰的测定 ,结果满意     

关于旅游产业中几种关系的探讨

总结归纳了旅游业中普遍存在的几种关系,介绍了这些关系的内涵、特征,最后提出协调几种关系的几点建议。     

一类多步Runge-Kutta方法的保单调性

在对步长作了一定的限制下研究了一类多步Runge-Kutta方法的保单调性,并得到了此类多步Runge-Kutta方法的保单调的充分条件,最后给出了试验方程y′=λ(t)y的情况.     

局部分析方法在可解群中的应用

局部分析方法是有限群理论最基本的方法,利用可解群的性质和有限群的基本定理,通过局部分析方法,研究了可解群和可解子群的一些性质.利用成分的性质及ThompsonA×B引理,得到了p-局部子群的相关结论.     

Preface

Different from traditional tax audit, supervision with self-audit is a combination of audit by the taxpayer himself and audit by the tax authority. This paper mainly studies a taxpayer’s optimal policy of tax evasion under supervision with self-audit and its related properties, in order to deduce some effective suggestions and theoretical bases to restrain tax evasion. Assuming that only a certain proportion of evaded tax can be discovered when the audit is executed, the authors first formulate a static model with self-audit. This model is divided into two stages. At the first stage, taxpayers declare their taxes, then the tax authority chooses audit objects, based on a known probability, and announces the result; if the taxpayer is chosen, he will enter the second stage, during which he has a chance to pay the evaded tax and the corresponding late fees and then is audited by the tax authority. The authors show the existence and uniqueness of the optimal amount of tax evasion at the first stage and the optimal proportion to self-expose at the second stage. The authors also discuss the related properties of the interior solution, and do elasticity analyses on some parameters. Besides, the authors extend the static model into the corresponding two-period model, and study the existence and uniqueness of the solution of the extended model. Finally, under the assumption that tax evasion can only be discovered with a certain probability when the audit is executed, the authors formulate another static model with self-audit and investigate its properties. This research is partially supported by the National Natural Science Foundation of China under Grant Nos. 60674082, 70221001, and 70731003.     

客户知识分类与共享方法分析

从客户知识的存储单位和可呈现性两个纬度对客户知识进行了分类,同时对其共享方法进行了分析。