压力补偿数字阀及系统数学模型,静动态数字仿真

本文以我校研制的PDV-Ⅲ型、 PDV-Ⅰ型数字阀及其组成系统为对象,建立了它们的数学模型,提出了一种解液压系统非线性微分方程的实用型方法——变微分方程等数法,并以阶跃和脉冲输入,进行了动态数字仿真。所得的结论,具有一定的指导意义。     

Characteristics of the binding features of Nelin with F-actin and screening Nelin interactive proteins

The interaction of nelin, a cardiac-specific expressed protein of human novel gene nelin, with F-actin was studied by both F-actin cosedimentation in vitro and colocalization assays. The results showed that nelin is a new F-actin binding protein and is colocolized with F-actin in cytoplasm of cells. Three new nelin binding proteins, filamin C subtype, titin N2B subtype and inter-alpha trypsin inhibitor heavy chain precursor (ITIH), were identified from human heart cDNA library using yeast two-hybrid screening. The binding activity of filamin C with nelin was confirmed by coimmunoprecipitation. Filamin C binds to nelin through its C-terminal region. It is indicated that nelin is a cytoskeleton associated protein and acts as a membrane-cytoskeleton associated protein involved in the formation of focal adhesions.     

Mismatch repair genes identified using genetic screens in Blm-deficient embryonic stem cells

Phenotype-driven recessive genetic screens in diploid organisms require a strategy to render the mutation homozygous. Although homozygous mutant mice can be generated by breeding, a reliable method to make homozygous mutations in cultured cells has not been available, limiting recessive screens in culture. Cultured embryonic stem (ES) cells provide access to all of the genes required to elaborate the fundamental components and physiological systems of a mammalian cell. Here we have exploited the high rate of mitotic recombination in Bloom's syndrome protein (Blm)-deficient ES cells to generate a genome-wide library of homozygous mutant cells from heterozygous mutations induced with a revertible gene trap retrovirus. We have screened this library for cells with defects in DNA mismatch repair (MMR), a system that detects and repairs base-base mismatches. We demonstrate the recovery of cells with homozygous mutations in known and novel MMR genes. We identified Dnmt1(ref. 5) as a novel MMR gene and confirmed that Dnmt1-deficient ES cells exhibit micro-satellite instability, providing a mechanistic explanation for the role of Dnmt1 in cancer. The combination of insertional mutagenesis in Blm-deficient ES cells establishes a new approach for phenotype-based recessive genetic screens in ES cells.     

Fingerprint representation methods based on B-Spline functions

The global characteristics of a fingerprint image such as the ridge shape and ridge topology are often ignored in most automatic fingerprint verification system.In this paper,a new representative method based on B-Spline curve is proposed to address this problem.The resultant B-Spline curves can represent the global characteristics completely and the curves are analyzable and precise.An algorithm is also proposed to extract the curves from the fingerprint image.In addition to preserve the most information of the fingerprint image,the knot-points number of the B-Spline curve is reduced to minimum in this algorithm. At the same time,the influence of the fingerprint image noise is discussed.In the end,an example is given to demonstrate the effectiveness of the representation method.     

Study on properties of residue-residue contacts in protein

Residue-residue contacts are very important in forming protein structure. In this work, we calculated the average probability of residue-residue contacts in 470 globular proteins and analyzed the distribution of contacts in the different interval of residues using Contacts of Structural Units (CSU) and Structural Classification (SCOP) software. It was found that the relationship between the average probability PL and the residue distance L for four structural classes of proteins could be expressed as lgPL=a+b x L, where a and b are coefficients. We also discussed the connection between two aspects of proteins which have equal array residue number and found that the distribution probability was stable (or un-stable) if the proteins had the same (or different) compact (for example synthase) in the same structural class.     

毫微秒脉冲发生器设计

利用晶体管的雪崩特性,本文设计制作可以按需要改变输出脉冲宽度的毫微秒脉冲发生器,可获得上升时间Ins、50Ω负载下最大输出脉冲幅度35V、脉冲成形宽度20ns、最大重复频率60kHz的毫微秒脉冲。     

璧固美矣犹堪琢之--教育部高教司组编98版《大学语文》指瑕

《大学语文》中出现释义欠妥、注音谬误及技术性错误,表明现行教材在编纂中略嫌粗放,亟待去伪存真,精雕细琢,使其日臻完善。     

应用Delphi4的MIDAS构件组开发起重机零部件管理系统

Delphi自版本4就开始提供一组新的构件MIDAS,使用它,可以方便地开发具有三层体系结构的数据库应用程序。本文分析了MIDAS构件组的开发原理,并以起重机为例,论述了开发基于三层结构的起重机零部件管理系统的基本步骤,并详细讨论了开发中三层结构的实现过程。     

履带式推土机粘弹性悬挂机构的开发研究

对履带式推土机行走机构的振动特性进行测试 ,建立起推土机缓冲系统模型 ,经对模型特性参数对传递率影响的分析 ,确定出参数的合理值域。把阻尼材料诺模图曲线数字化 ,做到了计算机对诺模图动态曲线的自动查询 ,实现了阻尼缓冲结构的动态优化。掌握了阻尼缓冲结构的设计理论和方法 ,已应用于 2 4 2kW履带式推土机粘弹性悬挂机构的开发。