N-terminal of L protein of vesicular stomatitis virus contains a new signal sequence

The L protein (241kD) of vesicular stomatitis virus (VSV) is the most important snbnnit of the replication complex. The existence of specific localization signal in the L protein was investigated by making recombinant constructs expressing truncated mutants of the L protein fused to green fluorescent protein (GFP) in transient transfection assays. The chimeric genes encoding varied N-terminal of L and GFP gene were put under the control of T7 promoter or CMV promoter. The fusion proteins were transiently expressed in BHK-21, COS-7, CHO or Hep G2 cells. When more than 120 residues were deleted or only 96 residues were kept on the N-terminal, the fusion proteins were shown to be distributed throughout the cells, cytoplasm and nucleus under the confocal microscope. However, other chimeric proteins with 120 or more amino acids were dotted and distributed in the perinuclear regions. And the fusion protein with 96—120 aa has the similar distribution. A thirteen-residue peptide QGYSFLHEVDKEA (108—120) was identified as localization signal, whose function would be absolutely distributed with the deficiency of D or V. Our results show that there is an independent localizing signal in N-terminal domain of L protein of VSV and this functional signal is conserved in different cell lines.     

Evolution of the South China Sea and monsoon history revealed in deep-sea records

As the third summary report of ODP Leg 184 to the South China Sea (SCS), this paper discusses the evolution of the East Asian monsoon and the SCS basin. A multi-proxy approach, involving geochemistry, micropale-ontology, pollen and other analyses, was adopted for reconstructing the evolutionary history of the East Asian monsoon, which was characterized by a series of paleo-climate events especially at 8, 3.2, 2.2 and 0.4 Ma. The new record indicates similar stages in the development of the East and South Asian monsoons, with an enhanced winter monsoon over East Asia being the major difference. The rich spectrums of monsoon variability from the southern SCS also reveal other characteristic features of the low latitude ocean. Evidence for the evolution of the SCS includes the hemipelagic Oligocene sediments, implying the existence of deep water environments during the early seafloor spreading stage of the SCS basin. The four major unconformities and some remarkabl ediagenetic features in upper Oligocene deposits indicate the strongest tectonic events in the region. From a careful comparison of lithologies and sedimentation rates, we conclude that the prominent differences in sedimentary environments between the southern and northern SCS were established only by ～3 Ma.     

鄂尔多斯盆地低渗透油田注水开发的调整与优化

根据鄂尔多斯盆地成功开发的主要特低渗油田——安塞、靖安、华池油田长6、长3油层的储层特征及开发实践,提出了改善特低渗油田注水开发效果的注采参数、注水方式优化及调整技术,从而达到提高单井产量及最终采收率,降低开发投资和生产成本,以提高整体开发效益的目的.     

尿囊素在润肤奶液中的应用

本介绍尿囊素在润肤奶液中的应用，以及尿囊素在该产品中的保湿效果和应用前景。     

试论达尔文进化论在生态学发展中的地位

介绍了现代生态学发展的特点和达尔文的进化论及完善过程，认为达尔文提出的生态思想是朴素的生态观，其进化论是种—种群的进化，从本质上并未涉及到其他生态学等级的进化；必须以普遍联系的系统观和时空观评价生态学发展现状，生态学研究也必须注重局部各因子之间、整体与局部之间的作用关系。初步探讨了能否象牛顿力学那样将自然选择力作为推动生物进化的“万有引力”的生态学研究问题。     

带宽控制技术分析及其实现

针对目前Internet技术中热门的QoS问题,讨论了带宽控制的基本方法和带宽动态划分的算法,分析了Linux内核和iproute2工具包中的关键数据结构,以及过滤器和队列调度规则,并对在Linux环境下,利用改进的双今牌桶算法对带宽的动态划分,实现对带宽的有效控制,从代码角度上作了分析和探索。     

SQL Server的安全性管理

介绍了SQL Server的安全机制．从进入系统到使用数据库对象，SQL Server都提供了比较完善的手段，方便系统管理员保护数据安全．     

复配型微乳液介质苯基荧光酮光度法测定痕量铅的研究

研究了在微乳液介质中 ,在pH =9.5时 ,铅与苯基荧光酮 (PF)显色生成稳定的 1:3配合物 ,在 5 72nm处摩尔吸光系数为 6 .0 4× 10 4L·mol-1·cm-1,铅含量在 0～ 1.2 μg/mL范围内符合比尔定律 ,采用巯基葡聚糖凝胶分离富集 ,消除了共存离子的干扰 ,用所拟方法测定水样中的微量铅 ,结果满意     

发展我国现代信息产业之浅见

从现代世界信息产业发展角度出发，结合我国信息产业发展的状况，从政府引导、合理调整产业结构、发展信息服务业、发挥联机信息检索作用、信息商品化、信息立法几方面提出了加速发展我国现代信息产业的对策与思考。     

高师声乐钢琴伴奏初探

钢琴伴奏是一门音乐表演艺术，在声乐伴奏中，它是配合歌唱成功的完成声乐作品的关键，本文分析、阐述、说明了钢琴伴奏在声乐演唱中的重要作用。