Two new Asian species of the genus Scottolana Huys, 2009 (Copepoda: Canuelloida: Canuellidae)

Two new species of the family Canuellidae Lang, 1944, Scottolana jasani sp. nov. collected from Gusipo Beach, Korea and S. huysi sp. nov. from Kata Beach, Phuket Island, Thailand, are described. So far 14 species are currently known in the genus, of which only two, S. bulbifera from Russia, China, and Korea, and S. geei from China, have been reported from Asia. The new species, Scottolana jasani sp. nov. and S. huysi sp. nov., belong to the longipes-group, defined by the presence of two postgenital segments in the female, and caudal ramus with a proximal hook-like inner projection. Scottolana jasani sp. nov. closely resembles S. geei. However, it is easily distinguished by the following characters: (1) number of segments of antennal exopod (8 in the new species, but 9 in S. geei); (2) setae I, and II of caudal ramus; (3) shape of genital field in both sexes; (4) segmentation of mandibular exopod; and (5) spinular ornamentation of endopod-2 in P1. Scottolana huysi sp. nov. is also closely related to S. geei. However, it is unique and different from S. geei by (1) indistinctly 4-segmented antennule in the female; (2) 5-segmented male antennule; (3) larger genital field in female; (4) inner long seta on P4 coxa; and (5) the morphology of caudal seta II, being long and plumose, with a bulbiform base in the female only.

www.zoobank.org/urn:lsid:zoobank.org:pub:33BFE1A2-8C95-4A9D-9E7A-B495CEEC3182     

Regulation of PDGF signalling and vascular remodelling by peroxiredoxin II

Platelet-derived growth factor (PDGF) is a potent mitogenic and migratory factor that regulates the tyrosine phosphorylation of a variety of signalling proteins via intracellular production of H2O2 (refs 1, 2-3). Mammalian 2-Cys peroxiredoxin type II (Prx II; gene symbol Prdx2) is a cellular peroxidase that eliminates endogenous H2O2 produced in response to growth factors such as PDGF and epidermal growth factor; however, its involvement in growth factor signalling is largely unknown. Here we show that Prx II is a negative regulator of PDGF signalling. Prx II deficiency results in increased production of H2O2, enhanced activation of PDGF receptor (PDGFR) and phospholipase Cgamma1, and subsequently increased cell proliferation and migration in response to PDGF. These responses are suppressed by expression of wild-type Prx II, but not an inactive mutant. Notably, Prx II is recruited to PDGFR upon PDGF stimulation, and suppresses protein tyrosine phosphatase inactivation. Prx II also leads to the suppression of PDGFR activation in primary culture and a murine restenosis model, including PDGF-dependent neointimal thickening of vascular smooth muscle cells. These results demonstrate a localized role for endogenous H2O2 in PDGF signalling, and indicate a biological function of Prx II in cardiovascular disease.     

Selective depletion of spinal noradrenaline inhibits post-decapitation convulsions in rats

Summary Local administration of 6-hydroxydopamine in the subarachnoidal space of the spinal cord in rats resulted in a selective long-lasting depletion of spinal noradrenaline, but not of dopamine, and prevented the occurrence of postdecapitation convulsions.     

Clinical and biochemical analysis of gluten toxicity I

Zusammenfassung Eine saure Peptid-Fraktion wurde aus dem Weizenprotein Gliadin durch Behandlung mit Pepsin und Trypsin erhalten. Das Verdauungsprodukt wurde durch Präzipitation mit Aceton und Adsorptionschromatographie an saurem Al₂O₃ weiter aufgearbeitet.Die erhaltene saure Peptid-Franktion wurde mit Perameisensäure nach dem vonSanger entdeckten Verfahren behandelt. Es wurde festgestellt, dass der in idiopathischer Steatorrhoe toxische Faktor nach dieser Behandlung noch immer vorlag.     

盐碱胁迫对抗碱盐生植物碱地肤生长特性的影响

以天然盐生植物碱地肤为材料,利用人工生态模拟方法对其施加0～400mmol·L^-1的盐胁迫（摩尔比1：1的NaCl和Na2SO4）和碱胁迫（摩尔比1：1的NaHCO3和Na2CO3）．通过测定其相对生长率（RGR）等生理响应指标分析其抗盐、抗碱能力及特点,以期明确其生态定位的生理基础．结果表明：碱地肤不仅具有强抗盐性也具有强抗碱性,在高达400mmol·L^-1的盐胁迫或400mmol·L^-1的碱胁迫下仍能存活并维持一定的生长．低浓度的盐胁迫（80mmol·L^-1）对其生长非但不抑制反而具有刺激作用．从RGR等胁变指标来看,在相同盐浓度下,碱胁迫对碱地肤的胁迫强度大于盐胁迫,碱地肤对碱胁迫所做出的胁变反应均大于对盐胁迫的反应．以此可推断,碱胁迫甚于盐胁迫,碱地肤的抗盐性大于抗碱性．     

时滞偏微分方程系统的稳定性检验

TDPDE系统的稳定性涉及到2D拟多项式, TDPDE系统的特征多项式为2D拟多项式,而其零点为一些连续的超曲面, 不再是孤立的和可分离的. 这导致检验TDPDE系统的稳定性非常困难. 为解决上述问题,提出一种检验TDPDE系统渐近稳定性的方法,该方法通过检验TDPDE系统对应的2D特征多项式的Hurwitz稳定性来确定TDPDE系统的渐近稳定性. 本文提出的定理建立了TDPDE系统的渐近稳定性与对应的2D特征多项式的Hurwitz稳定性关系, 提供了2D特征多项式(2D拟多项式)的Hurwitz稳定性检验方法. 由该文结果导出具有简单检验过程的2D拟多项式的Hurwitz-Schur稳定性数值检验算法,并用实例说明其应用。     

Laser hardening processing on tool steel SKD61

This paper is aimed at overcoming different degree defects of crystalline structure in SKD61 carbide tool steel under quenching in solid phase and liquid phase. The paper studies the micro-structure an...     

A conditional knockout resource for the genome-wide study of mouse gene function

Gene targeting in embryonic stem cells has become the principal technology for manipulation of the mouse genome, offering unrivalled accuracy in allele design and access to conditional mutagenesis. To bring these advantages to the wider research community, large-scale mouse knockout programmes are producing a permanent resource of targeted mutations in all protein-coding genes. Here we report the establishment of a high-throughput gene-targeting pipeline for the generation of reporter-tagged, conditional alleles. Computational allele design, 96-well modular vector construction and high-efficiency gene-targeting strategies have been combined to mutate genes on an unprecedented scale. So far, more than 12,000 vectors and 9,000 conditional targeted alleles have been produced in highly germline-competent C57BL/6N embryonic stem cells. High-throughput genome engineering highlighted by this study is broadly applicable to rat and human stem cells and provides a foundation for future genome-wide efforts aimed at deciphering the function of all genes encoded by the mammalian genome.