TMEM16A confers receptor-activated calcium-dependent chloride conductance

Calcium (Ca(2+))-activated chloride channels are fundamental mediators in numerous physiological processes including transepithelial secretion, cardiac and neuronal excitation, sensory transduction, smooth muscle contraction and fertilization. Despite their physiological importance, their molecular identity has remained largely unknown. Here we show that transmembrane protein 16A (TMEM16A, which we also call anoctamin 1 (ANO1)) is a bona fide Ca(2+)-activated chloride channel that is activated by intracellular Ca(2+) and Ca(2+)-mobilizing stimuli. With eight putative transmembrane domains and no apparent similarity to previously characterized channels, ANO1 defines a new family of ionic channels. The biophysical properties as well as the pharmacological profile of ANO1 are in full agreement with native Ca(2+)-activated chloride currents. ANO1 is expressed in various secretory epithelia, the retina and sensory neurons. Furthermore, knockdown of mouse Ano1 markedly reduced native Ca(2+)-activated chloride currents as well as saliva production in mice. We conclude that ANO1 is a candidate Ca(2+)-activated chloride channel that mediates receptor-activated chloride currents in diverse physiological processes.     

TiO_2薄膜光催化灭菌性能表征

以日用荧光灯和自然光为照射光源 ,研究了TiO2 薄膜的光催化灭菌性能 ,对比了具有不同紫外 可见吸收边波长薄膜的灭菌性能 .结果显示 ,在日用荧光灯照射下吸收边波长在 384nm的TiO2 薄膜具有较优的灭菌性能 ,而吸收边波长为 2 96nm的薄膜未显示明显的灭菌效果 .在室外自然光条件下 ,薄膜显示了灭菌性能 .