IFT54 regulates IFT20 stability but is not essential for tubulin transport during ciliogenesis

Intraflagellar transport (IFT) is required for ciliogenesis by ferrying ciliary components using IFT complexes as cargo adaptors. IFT54 is a component of the IFT-B complex and is also associated with cytoplasmic microtubules (MTs). Loss of IFT54 impairs cilia assembly as well as cytoplasmic MT dynamics. The N-terminal calponin homology (CH) domain of IFT54 interacts with tubulins/MTs and has been proposed to transport tubulin during ciliogenesis, whereas the C-terminal coiled-coil (CC) domain binds IFT20. However, the precise function of these domains in vivo is not well understood. We showed that in Chlamydomonas, loss of IFT54 completely blocks ciliogenesis but does not affect spindle formation and proper cell cycle progression, even though IFT54 interacts with mitotic MTs. Interestingly, IFT54 lacking the CH domain allows proper flagellar assembly. The CH domain is required for the association of IFT54 with the axoneme but not with mitotic MTs, and also regulates the flagellar import of IFT54 but not IFT81 and IFT46. The C-terminal CC domain is essential for IFT54 to bind IFT20, and for its recruitment to the basal body and incorporation into IFT complexes. Complete loss of IFT54 or the CC domain destabilizes IFT20. ift54 mutant cells expressing the CC domain alone rescue the stability of IFT20 and form stunted flagella with accumulation of both IFT-A component IFT43 and IFT-B component IFT46, indicating that IFT54 also functions in IFT turn-around at the flagellar tip.     

Melatonin and mitochondrial function during ischemia/reperfusion injury

Ischemia/reperfusion (IR) injury occurs in many organs and tissues, and contributes to morbidity and mortality worldwide. Melatonin, an endogenously produced indolamine, provides a strong defense against IR injury. Mitochondrion, an organelle for ATP production and a decider for cell fate, has been validated to be a crucial target for melatonin to exert its protection against IR injury. In this review, we first clarify the mechanisms underlying mitochondrial dysfunction during IR and melatonin’s protection of mitochondria under this condition. Thereafter, special focus is placed on the protective actions of melatonin against IR injury in brain, heart, liver, and others. Finally, we explore several potential future directions of research in this area. Collectively, the information compiled here will serve as a comprehensive reference for the actions of melatonin in IR injury identified to date and will hopefully aid in the design of future research and increase the potential of melatonin as a therapeutic agent.     

非下采样Contourlet HMT模型

非下采样Contourlet变换（NSCT）不仅具有类似传统小波变换和Contourlet变换的多尺度和多方向特性,而且还具有良好的平移不变特性．文中对NSCT变换系数进行研究,获得NSCT变换的方向子带系数同样具有持续性和聚集性的结论;在此基础上结合隐Markov树模型,建立了一种新的基于NSCT域的隐Markov树模型NSCT—HMT,并且给出了该模型的参数结构以及模型参数的训练方法;最后将所提出的模型应用于图像去噪中取得了很好的仿真效果．     

探月工程三期月地高速再入返回飞行器防热系统设计与验证

本文针对月地高速再入返回的高焓、二次大气层再入、高热流密度峰值与长加热时间耦合的气动加热环境特点,提出了飞行器防热系统设计方案,研制了七种新型碳硅复合烧蚀防热材料,建立了防热系统分析模型,并完成了地面试验验证.经对飞行试验数据的详细分析,结果表明防热系统的实际性能与设计预期一致.     

A genetic interaction network of five genes for human polycystic kidney and liver diseases defines polycystin-1 as the central determinant of cyst formation

Autosomal dominant polycystic liver disease results from mutations in PRKCSH or SEC63. The respective gene products, glucosidase IIβ and SEC63p, function in protein translocation and quality control pathways in the endoplasmic reticulum. Here we show that glucosidase IIβ and Sec63p are required in mice for adequate expression of a functional complex of the polycystic kidney disease gene products, polycystin-1 and polycystin-2. We find that polycystin-1 is the rate-limiting component of this complex and that there is a dose-response relationship between cystic dilation and levels of functional polycystin-1 following mutation of Prkcsh or Sec63. Reduced expression of polycystin-1 also serves to sensitize the kidney to cyst formation resulting from mutations in Pkhd1, the recessive polycystic kidney disease gene. Finally, we show that proteasome inhibition increases steady-state levels of polycystin-1 in cells lacking glucosidase IIβ and that treatment with a proteasome inhibitor reduces cystic disease in orthologous gene models of human autosomal dominant polycystic liver disease.     

基于非等量采样的伪码同步技术研究

针对数字通信系统中伪随机噪声(pseudo-random noise, PN)码同步技术的同步精度受限于采样间隔的问题,结合可变群时延的高精度同步思想,利用采样点位置偏移量的变化,推导出了非等量采样(non-commensurate sampling, NCS)下的PN码相关函数解析表达式,提出了基于精度因子的NCS率选取准则,通过精度因子可快速判断出NCS后PN码的同步精度下限。在此基础上,分析了码序列周期、接收机前端滤波器等附加因素对PN码同步精度的影响。对NCS率选取准则的研究,为优化数字通信系统设计提供了理论指导,达到以低硬件消耗实现高精度同步的目的。     

基于装备体系韧性的作战网络链路重要度评估及恢复策略

韧性可以全面描述装备体系吸收打击或干扰、并从打击或干扰中恢复的能力, 体现了体系的综合性能。针对装备体系的韧性提出了一种量化评价方法, 以对装备体系作战网络链路进行重要度评估。首先给出了装备体系韧性的明确定义, 并建立了量化韧性的数学模型; 其次提出了基于韧性增加值的链路重要度指标, 并构建了一种基于装备体系韧性的作战网络链路重要度评估模型; 然后构建了不同的恢复策略,并分析了其对作战网络性能恢复的影响; 最后通过应用案例验证了所提方法与模型的适用性和合理性, 为优化体系结构、保障和提高体系作战性能提供了理论支撑, 也为进一步开展基于装备体系韧性的作战网络应用研究提供了参考借鉴。     

四川盆地地表水酸化现状与趋势

2009～2011年间对四川盆地地表水的酸化现状进行了一次大规模调查.通过对采集的90多个地表水样的pH和主要阴阳离子浓度的分析发现,除了重庆铁山坪溪流的pH低于6.0,万州开县天蛾池、重庆铁山坪溪流和峨眉山金顶池塘等少数地表水的酸中和容量(ANC)低于0.200mmoleL1,从而表明这些地表水体可能已经发生严重酸化之外,其他水体均具有较高的pH和ANC,表明在四川盆地地表水酸化可能并非严重的区域环境问题.这里存在30多年的严重酸沉降污染,部分水体与20多年前的调查结果相比存在明显的酸化趋势,表现为ANC显著下降,同时SO42和NO3浓度显著升高.SO2和NOx排放的增长是地表水酸化的原因,其中SO2的贡献更明显.四川盆地广泛分布的紫色土具有较高的化学风化速率,是地表水具有较强的酸中和容量的原因.而在周边山地分布着黄壤等风化速率较低的土壤类型,严重的酸沉降可能导致个别地表水的酸化.     

Non-Uniform Doo-Sabin Subdivision Surface via Eigen Polygon

This paper constructs a new non-uniform Doo-Sabin subdivision scheme via eigen polygon.The authors proved that the limit surface is always convergent and is G1 continuous for any valence and any positive knot intervals under a minor assumption, that λ is the second and third eigenvalues of the subdivision matrix. And then, a million of numerical experiments are tested with randomly selecting positive knot intervals, which verify that our new subdivision scheme satisfies the assumption.However this is not true for the other two existing non-uniform Doo-Sabin schemes in Sederberg, et al.(1998), Huang and Wang(2013). In additional, numerical experiments indicate that the quality of the new limit surface can be improved.