高效液相色谱法测定清喉炎合剂及冲剂中黄芩甙含量

用反相高效液相色谱法对清喉炎合剂和冲剂中的黄芩甙含量进行测定．固定相柱为Ｓｈｉｍ－ｐａｃｋＣＬＣ－ＯＤＳ（１５０ｍｍ×６ｍｍ,５μｍ）;流动相为甲醇－水－磷酸（４７∶５３∶０．２）,检测波长２７７ｎｍ．方法平均回收率及ＲＳＤ分别为９９．４％和２．３％．     

路段通行能力的动态微观仿真研究

用动态微观仿真方法研究路段通行能力,从微观的角度出发,考虑驾驶员,车辆,道路, 环境和交通规划的相互关系对通行能力的影响并建立了仿真模型。     

ATM广域网预测式流量控制方案

针对ＡＴＭ广域网的长时延特点,提出了一种预测式流量控制方案,通过预测各交换节点非受控业务流所占用的带宽,对各链路可获得带宽进行预测式分配;预测式方案结合本文作者提出的集中式流量控制机制,保证了带宽分配的公平性和链路利用的高效性;本文还在ＶＢＲ＋ＡＢＲ业务综合传输的情形下,进行了预测式流量控制方案的计算机仿真,仿真结果表明,本文的预测式算法具有带宽分配的公平性和链路利用的高效性,交换节点处缓冲队列很     

Genomic organization of EDSV strain AA-2

The genomic organization of egg drop syndrome virus strain AA-2 isolated in China is reported here. The genome was found to be 32 838 bp in length, approximately 2 kb shorter than those of the human subgenus C adenoviruses. Analysis of open reading frames indicated that the genes for major viral structural proteins (55 K, Penton base, pⅦ, pⅩ, pⅥ, Hexon, 100 K, pⅧ and Fiber), as well as EP, DNA polymerase and ⅣaⅡ are present in the expected locations in the genome. EDSV possesses no identifiable E3 and E4 regions and most proteins encoded by E3 and E4 regions of other adenoviruses genome were not presented in EDSV.     

A continuous 200-ka palaeoclimatic record from stalagmite in Tangshan Cave, Nanjing

An oxygen isotope temperature record from 381 to 166 kaB. P. (²³⁰Th/²³⁴U dating and tuned ages) has been obtained by combining data on the isotopic composition of calcites with that of fluid inclusions trapped in a stalagmite from a limestone cave in which a fossil cranium deposited. Several laminae in microsequence of the stalagmite represent climatic events or shifts. The Nanjing man fossil was older than 381 kaB. P.     

Cloning and expression of the vp39 gene ofBombyx mori nuclear polyhedrosis virus inE. coli

The nuclear capsid protein gene (vp39) ofBombyx mori nuclear polyhedrosis virus (BmNPV) was amplified successfully by PCR technique and inserted into pGEM 3zf(+). The 5′ and 3′ terminal area of the amplified vp39 gene were sequenced with silver-staining dideoxy method. Bmvp39 gene was sub-cloned into the expression vector pRSET-A, and transformed intoE. coli BL21. This gene was highly expressed by IPTG induction. SDS-PAGE analysis showed that the expressed protein is about 38 kd, and the expressed amount reached maxium in 4 h with IPTG induction. Supported by the National Natural science Foundation of China and the Doctoral Foundation of Edn carto Committee Liu Deli: born in 1954, Doctoral Candidate from Huazhong Normal University To whom correspondence should be addressed: (027-7882712-2938)     

花生油磷酸酯加脂剂的研制及应用

本文报道了以花生油为原料,经酯交换,磷酸化制备新型皮革加脂剂,并研究了其单一及复配后的加脂性能。     

马尔柯夫链状预测的概率计算及拓广

提出了一种马尔柯夫链状预测中的概率计算新方法：状态划分矩阵法;具有模糊状态的马尔柯夫链的概率计算公式,并用实例计算作了说明。     

腺病毒载体介导胸苷激酶基因/ACV对大鼠脑胶质瘤的离体和活体杀伤作用

建立缺陷型重组腺病毒载体介导的胸苷激酶基因治疗系统（Adtk／ACV）,进行离休和活体治疗大鼠C6脑胶质瘤研究．将质粒pAdtk与pJM17共转染腺病毒包装细胞──293细胞,同源重组后制备纯化的Adtk并以PCR证实;Adtk对离休C6细胞的杀伤作用随着Adtk滴度和ACV剂量增加而增强,并有旁观者效应,而未转染C6细胞和AdLacZ转染C6细胞均未被杀伤;扫描电镜下见Adtk／ACV处理的细胞呈明显病理改变;ACV可有效杀伤Adtk／C6细胞．在大鼠脑立体定向仪引导下于右额叶接种2×106个C6细胞,分别于第3,6,8和10天原位注射Adtk,同时腹腔注射ACV（100mg／（kg·d-1））,3d组、6d组、8d组大鼠成活期在90d以上,组织学检查未发现肿瘤细胞．10d组成活期（28．5±4．6）d,而C6未治疗组和AdLacZ／ACV治疗组成活期分别为（1．8±3．1）d和（14．0±2．2）d．本文建立的Adtk／ACV在离体和活体水平对大鼠C6脑胶质瘤有强烈的杀伤作用,效果确实,应用方便,ACV价格便宜,有潜在临床应用价值．     

南方红豆杉扦插繁殖技术研究Ⅱ.穗条对扦插生根率的影响

本文系统研究了南方红豆杉(Taxus chinensisvar.mairei)扦插育苗中穗条来源对生根率的效应。结果显示,采条母树龄、穗条年龄和穗条采自的树冠部位均对扦插生根率影响较大。本研究3年龄实生苗优于胸径10-20 cm的母树,后者又优于胸径30-40 cm的母树,胸径50 cm以上的最差,且差异显著。在母树龄和树冠部位一样的条件下,1年龄穗条的生根率显著高于2年龄穗条,树冠中部穗条优于上部,下部穗条生根率最低。采用3年龄实生苗为采条母树,并采集树冠中部的1年龄穗条,其最高生根率可达98.7%。研究结果为扦插繁殖中穗条的选择提供科学依据。