Functional analysis of an archaebacterial voltage-dependent K+ channel

All living organisms use ion channels to regulate the transport of ions across cellular membranes. Certain ion channels are classed as voltage-dependent because they have a voltage-sensing structure that induces their pores to open in response to changes in the cell membrane voltage. Until recently, the voltage-dependent K+, Ca2+ and Na+ channels were regarded as a unique development of eukaryotic cells, adapted to accomplish specialized electrical signalling, as exemplified in neurons. Here we present the functional characterization of a voltage-dependent K+ (K(V)) channel from a hyperthermophilic archaebacterium from an oceanic thermal vent. This channel possesses all the functional attributes of classical neuronal K(V) channels. The conservation of function reflects structural conservation in the voltage sensor as revealed by specific, high-affinity interactions with tarantula venom toxins, which evolved to inhibit eukaryotic K(V) channels.     

The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria

Bacillus anthracis is an endospore-forming bacterium that causes inhalational anthrax. Key virulence genes are found on plasmids (extra-chromosomal, circular, double-stranded DNA molecules) pXO1 (ref. 2) and pXO2 (ref. 3). To identify additional genes that might contribute to virulence, we analysed the complete sequence of the chromosome of B. anthracis Ames (about 5.23 megabases). We found several chromosomally encoded proteins that may contribute to pathogenicity--including haemolysins, phospholipases and iron acquisition functions--and identified numerous surface proteins that might be important targets for vaccines and drugs. Almost all these putative chromosomal virulence and surface proteins have homologues in Bacillus cereus, highlighting the similarity of B. anthracis to near-neighbours that are not associated with anthrax. By performing a comparative genome hybridization of 19 B. cereus and Bacillus thuringiensis strains against a B. anthracis DNA microarray, we confirmed the general similarity of chromosomal genes among this group of close relatives. However, we found that the gene sequences of pXO1 and pXO2 were more variable between strains, suggesting plasmid mobility in the group. The complete sequence of B. anthracis is a step towards a better understanding of anthrax pathogenesis.     

黄河沉积物中重金属离子的形态转化及释放研究

以黄河包头段上游清洁河段的沉积物为吸附剂,以Pb^2 、Cu^2 、Zn^2 、Cd^2 等多离子溶液为吸附质,开展了重金属离子被黄河沉积物吸附后的再释放,以及吸附作用对重金属形态转化的影响等实验研究．结果表明,重金属离子被黄河沉积物吸附后,各元素均不转入残渣态,Cu^2 和Zn^2 主要向碳酸盐结合态及铁锰氧化物结合态转化,Pb^2 主要向碳酸盐结合态和可交换态转化;Cd^2 主要转入可交换态和碳酸盐结合态．吸附后的赋存形态决定了Cd^2 的释放量及释放能力远远大于其它3种重金属离子,由Cd^2 排放引起的污染不易消除且影响长久．     

Fluorescence spectra and enzymatic property of hemoglobin as mimetic peroxidase

0　IntroductionHemoglobin(Hb)isthemajorhemeproteinofredbloodcells(RBCs)andisresponsibleforthetransportofoxygentothetissues.ThefunctionofHbdependsupontheabilityoffer rousironinthehemegrouptobindandreleaseoxygen .Despiteitsprincipalroleasanoxygen carrier,theHbmoleculepossessesdifferentenzymaticactivities[1 ] andamethodwasdevelopedforthedeterminationofHbbasedonitsenzymaticactivityfortheox idationofo phenylenediamine (OPDA)withH2 O2 asanoxi dant[2 ] .Hbasamimeticenzymeofperoxidase ,cancataly…     

Quick trickle permutation based on quick trickle characteristic sequence

0　IntroductionAblockcipherwithoutdataextensionorexpressionisaper mutation .Thesecurityofablockcipherschemeiscorrela tivewiththe permutations.Quicktrickle permutation[1 ] canmakethedatadisorderedwhenactingatthedataandmakeallprobabledistancebetweentwoelementsappear,soithasgoodcryptographicpropertiesandcanbeusedintheblockcipherwhichrequireshighsecurity.Aprivatekeyblockcipherisapermutationinn dimensionvectorspaceoverF2 atthecontrolofakey[2 ,3] .Moreover,manyblockciphersusepermutationstoexecuteso…     

Design and implementation of WebGIS based on SOAP

0　IntroductionTheWorldWideWebisconsideredasalargedistributedin formationsystemthatprovidesaccesstoshareddataob jects.AsoneofthemostpopularapplicationscurrentlyrunningontheInternet,theWorldWideWebisofanexponentialgrowthinsize,whichresultsinnetworkcongestionandserveroverloading .Inthefieldsofspatialinformationsystems,therehavethesameproblems.Recently ,attentionhasbeenpaidtoefficientintegra tionoftheWebandGIS(GeographicInformationSystem)appli cation .ThisisbecausetheWebhasmanyadvantages,i…     

Research on data combination for Phased-array Ground Penetrating Radar

0　IntroductionGroundPenetratingRadar (GPR)isanewandefficientex ploringmeanstodetecttheobjectinshallowsubsurface.Comparedwithotherdetectingmethods,GPRhasmanyadvan tages,suchasquickness,highresolution ,convenientoperationetc.SoGPRisappliedtonationaldefenseandnationaleconomy .IndevelopmentofGPRtechnology ,therecomeforthcontinuous waveradar,FMcontinuous waveradar,andshockpulseradar.Amongoftheseradars,shockpulseradarsendsgreatamplitudeimpulsewithperiodinns,receivingequivalentsamplingtoreali…     

Application of support vector machines regression in prediction Shanghai stock composite index

0　IntroductionNeuralnetworks,inrecentyears,havebeensuccessfullyusedformodelingfinancialtimeseries.Researchesrevealneuralnetworkshavebetterresultsindescribingdynamiccharac teristicscomparingwithclassicalstatisticalmethods[1 3] .Back propagation (BP)neuralnetworkisamostpopularlyusedneuralnetworkmodelintheareaoffinancialforecasting .AlthoughBP’sstructureissimple ,ithaspowerfulabilityinsolvingvariousproblems.TherearealsoshortcomingswithBP :needsformanycontrolparameters,difficultyingettingsta…     

Investigation on the coordinate structures of the rare earth metal complexes with edta and cydta ligands

0　IntroductionThegreatattentionhasbeenpaidtorareearthorradioactiverareearthmetalcomplexesfortheirvariousbiologicactivi ties[1 3] .Forexamples,theradioactiverareearthmetal1 53SmⅢ com plexeswerewidelyusedfortumourtherapyofbrain ,liver,lung ,heartandbonetissues[4 5] ;forrareearthmetalEuⅢandTbⅢemitinguniquefluorescence ,theircompoundswereappliedfordiagnosesofvariousdiseases[6 ,7] ;forrareearthmetalGdⅢcontainingthemosthigh spinsingleelectrons,itscomplexeswereusuallyusedforcontrastagentsofmag…     

A CORBA-Based Multidatabase System——Panorama Project

0　ＩｎｔｒｏｄｕｃｔｉｏｎＴｈｅｎｅｅｄｆｏｒｉｎｔｅｇｒａｔｉｏｎｏｆｄｉｆｆｅｒｅｎｔｄａｔａｂａｓｅｍａｎａｇｅｍｅｎｔｓｙｓｔｅｍｓ (ＤＢＭＳｓ) ,ｍａｋｉｎｇｄｉｆｆｅｒｅｎｃｅｓｂｅｔｗｅｅｎｔｈｅｓｅｓｙｓｔｅｍｓｉｎｖｉｓｉｂｌｅａｎｄｐｒｏｖｉｄｉｎｇｕｓｅｒｓｗｉｔｈａｕｎｉｆｏｒｍａｃｃｅｓｓｔｏａｌｌｔｈｅｄａｔａｂａｓｅｓｈａｓｇｒｏｗｎｒａｐｉｄｌｙｄｕｒｉｎｇｔｈｅｌａｓｔｄｅｃａｄｅ .Ｈｏｗｅｖｅｒ,ｄａｔａｂａｓｅａｕｔｏｎｏｍｙａｎｄｈｅｔｅｒｏｇｅｎｅｉｔｙ…