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161.
Ectomycorrhizal colonization and rooting characteristics were quantified in a mature ponderosa pine ( Pinus ponderosa Dougl. ex Laws.) stand in the western Sierra Nevada. Root length totaled 3835.9 m · m –2 of forest floor surface area, with 96% consisting of the fine-root fraction. Total root dry weight and volume were 2230.4 g · m –2 and 5807.4 cm 3 · m –2 of forest floor area, respectively, with 69% of the former and 75% of the latter accounted for by the coarse fraction. Fine roots were most prevalent in the upper 15 cm of the mineral soil profile, and their abundance declined with increasing depth. Coarse roots were most abundant at a depth of 15–30 cm. Ectomycorrhizal counts totaled 26,814 · m –2 of forest floor area, and an overwhelming preponderance was associated with the fine-root fraction. More than three-quarters of mychorrhizae resided in the upper 15 cm of mineral soil, with an overall trend of declining numbers with increasing depth. Roots and mycorrhizae were exceedingly scarce at a depth of 45–60 cm, and neither was found in the organic soil layer above the mineral profile. A necessary step in understanding the ecophysiological role of mycorrhizae in mature forests is to quantify their abundance in such settings, and the results of this study contribute such information for ponderosa pine. 相似文献
162.
The effects of elevation and environmental parameters associated with elevation on the dinitrogen fixation rates, N 2 [C 2 H 2 ], of a North American lupine species were evaluated. Lupinus argenteus Pursh plants growing at five elevations on Francis Peak, Utah, were sampled weekly throughout the growing season. The highest elevation site had the greatest frequency of nodulated plants. Influence of the environmental and physiological variables on fixation activity varied with elevation and the mean time of sampling. Soil moisture, nodule dry weight, and air temperature were important. Less than one-half of the observed variation in fixation rates, N 2 [C 2 H 2 ], was accounted for by variation in the associated independent variables. Nevertheless this study documented that nodulated plants of L. argenteus were capable of actively fixing nitrogen at a range of elevations on Francis Peak. 相似文献
163.
Walker T Johnson PH Moreira LA Iturbe-Ormaetxe I Frentiu FD McMeniman CJ Leong YS Dong Y Axford J Kriesner P Lloyd AL Ritchie SA O'Neill SL Hoffmann AA 《Nature》2011,476(7361):450-453
Dengue fever is the most important mosquito-borne viral disease of humans with more than 50 million cases estimated annually in more than 100 countries. Disturbingly, the geographic range of dengue is currently expanding and the severity of outbreaks is increasing. Control options for dengue are very limited and currently focus on reducing population abundance of the major mosquito vector, Aedes aegypti. These strategies are failing to reduce dengue incidence in tropical communities and there is an urgent need for effective alternatives. It has been proposed that endosymbiotic bacterial Wolbachia infections of insects might be used in novel strategies for dengue control. For example, the wMelPop-CLA Wolbachia strain reduces the lifespan of adult A. aegypti mosquitoes in stably transinfected lines. This life-shortening phenotype was predicted to reduce the potential for dengue transmission. The recent discovery that several Wolbachia infections, including wMelPop-CLA, can also directly influence the susceptibility of insects to infection with a range of insect and human pathogens has markedly changed the potential for Wolbachia infections to control human diseases. Here we describe the successful transinfection of A. aegypti with the avirulent wMel strain of Wolbachia, which induces the reproductive phenotype cytoplasmic incompatibility with minimal apparent fitness costs and high maternal transmission, providing optimal phenotypic effects for invasion. Under semi-field conditions, the wMel strain increased from an initial starting frequency of 0.65 to near fixation within a few generations, invading A. aegypti populations at an accelerated rate relative to trials with the wMelPop-CLA strain. We also show that wMel and wMelPop-CLA strains block transmission of dengue serotype 2 (DENV-2) in A. aegypti, forming the basis of a practical approach to dengue suppression. 相似文献
164.
165.
Quantum annealing with manufactured spins 总被引:1,自引:0,他引:1
Johnson MW Amin MH Gildert S Lanting T Hamze F Dickson N Harris R Berkley AJ Johansson J Bunyk P Chapple EM Enderud C Hilton JP Karimi K Ladizinsky E Ladizinsky N Oh T Perminov I Rich C Thom MC Tolkacheva E Truncik CJ Uchaikin S Wang J Wilson B Rose G 《Nature》2011,473(7346):194-198
Many interesting but practically intractable problems can be reduced to that of finding the ground state of a system of interacting spins; however, finding such a ground state remains computationally difficult. It is believed that the ground state of some naturally occurring spin systems can be effectively attained through a process called quantum annealing. If it could be harnessed, quantum annealing might improve on known methods for solving certain types of problem. However, physical investigation of quantum annealing has been largely confined to microscopic spins in condensed-matter systems. Here we use quantum annealing to find the ground state of an artificial Ising spin system comprising an array of eight superconducting flux quantum bits with programmable spin-spin couplings. We observe a clear signature of quantum annealing, distinguishable from classical thermal annealing through the temperature dependence of the time at which the system dynamics freezes. Our implementation can be configured in situ to realize a wide variety of different spin networks, each of which can be monitored as it moves towards a low-energy configuration. This programmable artificial spin network bridges the gap between the theoretical study of ideal isolated spin networks and the experimental investigation of bulk magnetic samples. Moreover, with an increased number of spins, such a system may provide a practical physical means to implement a quantum algorithm, possibly allowing more-effective approaches to solving certain classes of hard combinatorial optimization problems. 相似文献
166.
Structural changes in glycogen phosphorylase induced by phosphorylation 总被引:27,自引:0,他引:27
S R Sprang K R Acharya E J Goldsmith D I Stuart K Varvill R J Fletterick N B Madsen L N Johnson 《Nature》1988,336(6196):215-221
A comparison of the refined crystal structures of dimeric glycogen phosphorylase b and a reveals structural changes that represent the first step in the activation of the enzyme. On phosphorylation of serine-14, the N-terminus of each subunit assumes an ordered helical conformation and binds to the surface of the dimer. The consequent structural changes at the N- and C-terminal regions lead to strengthened interactions between subunits and alter the binding sites for allosteric effectors and substrates. 相似文献
167.
Cross A. J. Crow T. J. Johnson J. A. 《Cellular and molecular life sciences : CMLS》1983,39(10):1168-1171
Summary Incubation of calf brain membrane preparations with the plant lectins, concanavalin A and wheat germ agglutinin did not inhibit neurotransmitter receptor binding sites directly. Plant lectins did however protect these sites against subsequent trypsin digestion suggesting that neurotransmitter binding sites may be associated with glycoprotein structures. 相似文献
168.
J. L. Fausnaugh J. F. Blazyk S. C. El-Saleh P. Johnson 《Cellular and molecular life sciences : CMLS》1984,40(1):83-84
Summary Differential scanning calorimetry of polymeric F-actin at pH 8.0 showed that the polymer had a concentration-independent thermal profile with a single transition temperature of 81 °C. In contrast, the thermal profile of G-actin was concentration-dependent, and although it resembled the F-actin profile at lower concentrations, it was found to have a more complex profile at higher protein concentrations.Acknowledgment. This research was carried out under grants to J.F.B. and P.J. from The Muscular Dystrophy Association of America. 相似文献
169.
The muscle enzyme calpain II, in contrast to muscle calpain I, was markedly inhibited by millimolar concentrations of the polyamines spermine and spermidine. These compounds and the calpain inhibitor calpastatin had synergistic inhibitory effects on calpain II. These results suggest that the polyamines may have possible regulatory effects on the in vivo activity of calpain II enzymes. 相似文献
170.
The cDNA sequence of the precursor of mouse epidermal growth factor (EGFP) has recently been reported by two groups, both of whom noted the presence of repeated similar segments, each about 40 residues long. One of these repeat units overlaps with the sequence of epidermal growth factor itself. The sequence of epidermal growth factor has been reported to be similar to that of pancreatic secretory trypsin inhibitor (PSTI) and a somewhat better match has been found with part of the sequence of bovine factor X, one of the blood coagulating factors. We report here that there is an even stronger similarity between the sequences of some of the repeat units of epidermal growth factor precursor and certain segments in factor X. This sequence similarity is also apparent in comparisons with other blood coagulation factors. On the basis of these sequence comparisons we suggest a scheme for the evolution of the epidermal growth factor precursor. We have also identified certain structural features in the precursor sequence that bear on the way in which the mature epidermal growth factor is generated. 相似文献