Urinary bladder hyporeflexia and reduced pain-related behaviour in P2X3-deficient mice

Extracellular ATP is implicated in numerous sensory processes ranging from the response to pain to the regulation of motility in visceral organs. The ATP receptor P2X3 is selectively expressed on small diameter sensory neurons, supporting this hypothesis. Here we show that mice deficient in P2X3 lose the rapidly desensitizing ATP-induced currents in dorsal root ganglion neurons. P2X3 deficiency also causes a reduction in the sustained ATP-induced currents in nodose ganglion neurons. P2X3-null mice have reduced pain-related behaviour in response to injection of ATP and formalin. Significantly, P2X3-null mice exhibit a marked urinary bladder hyporeflexia, characterized by decreased voiding frequency and increased bladder capacity, but normal bladder pressures. Immunohistochemical studies localize P2X3 to nerve fibres innervating the urinary bladder of wild-type mice, and show that loss of P2X3 does not alter sensory neuron innervation density. Thus, P2X3 is critical for peripheral pain responses and afferent pathways controlling urinary bladder volume reflexes. Antagonists to P2X3 may therefore have therapeutic potential in the treatment of disorders of urine storage and voiding such as overactive bladder.     

木果楝挥发油成分的GC-MS分析

采用气相色谱-质谱联用技术,首次对木果楝叶子和果实挥发油进行了成分分析,从木果楝叶子和果实分别鉴定出了33和36种化合物,分别占挥发油的99.11%和97.63%.两个部位中挥发油化学组成各有异同,但其主要成分均是倍半萜类化合物b-桉叶烯和γ-桉叶烯.本研究结果可为木果楝的综合开发利用提供理论依据.     

Circular object recognition based on shape parameters

To recognize circular objects rapidly in satellite remote sensing imagery, an approach using their geometry properties is presented. The original image is segmented to be a binary one by one dimension maximum entropy threshold algorithm and the binary image is labeled with an algorithm based on recursion technique. Then, shape parameters of all labeled regions are calculated and those regions with shape parameters satisfying certain conditions are recognized as circular objects. The algorithm is described in detail, and comparison experiments with the randomized Hough transformation (RHT) are also provided. The experimental results on synthetic images and real images show that the proposed method has the merits of fast recognition rate, high recognition efficiency and the ability of anti-noise and anti-jamming. In addition, the method performs well when some circular objects are little deformed and partly misshapen.     

Reverse Programmed SONOS Memory Technique for 0.18 μm Embedded Utilization

A 4 Mb embedded silicon-oxide-nitride-oxide-silicon (SONOS) memory was developed with a 0.18 μm CMOS logic compatible technology. A reverse programming array architecture was proposed to reduce the chip area, enhance the operating window, and increase the read speed. The charge distribution was analyzed to optimize the programming and erase conditions considering both the operating speed and the endurance performance. The final test chip has a good endurance of 105 cycles and a data retention time of at least 10 years.     

Banach空间值独立随机变量序列的Hàjek-Rènyi不等式

证明了Banach空间值独立随机变量序列的Hàjek-Rènyi型不等式,并利用该不等式证明了Banach空间值独立随机变量序列的强大数定律,所得结果刻画了Banach空间的p型性质.     

无刷新技术在多媒体会议系统中的应用与实现

在分析比较各种更新页面技术的基础上,利用Ajax的核心技术XMLHTTP组件,设计并实现了多媒体会议系统中无刷新页面的功能,它具有Web页面与后台数据库信息同步更新、响应速度快的特点.系统测试表明:此无刷新技术比其他技术具有更多的优越性.     

Field trial of an HTS filter system on a CDMA base station

This paper presents the field trial results of a high temperature superconductor filter system (HTSFS) in a code division multiple access (CDMA) mobile communication base station (BTS) in Tangshan, China. The center frequency of the HTSFS is 830 MHz with 1.4% fractional bandwidth. The noise figure (NF) of the HTSFS, including connectors and cables, is 0.8 dB. The field trial results show that after the HTSFS was installed in the receiver front-end of a CDMA base station, the transmitting power of handset was decreased by 3.1 dB on average. Up to now, the HTSFS has been running steadily in the base station for more than 22 months, demonstrating the reliability and stability of the system.     

2,3-Dihydroxybiphenyl dioxygenase gene was first discovered in Arthrobacter sp. strain PJ3

Bacterium strain PJ3,isolated from wastewater and identified as Arthrobacter sp. bacterium based on its 16S rDNA gene,could use carbazole as the sole carbon,nitrogen and energy source. The genomic library of strain PJ3 was constructed and a positive clone JM109(pUCW402) was screened out for the expression of dioxygenase by the ability to form yellow ring-fission product. A 2,3-dihydroxybiphenyl dioxygenase(23DHBD) gene of 933 bp was found in the 3360 bp exogenous fragment of pUCW402 by GenSCAN software and BLAST analysis. The phylogenetic analysis showed that 23DHBD from strain PJ3 formed a deep branch separate from a cluster containing most known 23DHBD in GenBank. Southern hybridization confirmed for the first time that the 23DHBD gene was from the genomic DNA of Arthrobacter sp. PJ3. In order to test the gene function,recombinant bacterium BL21(pETW-8) was constructed to express 23DHBD. The expression level in BL21(pETW-8) was highest compared with the recombinant bacteria JM109(pUCW402) and strain PJ3. We observed that 23DHBD was not absolute specific. The enzyme activity was higher with 2,3-dihydroxybiphenyl as a substrate than with catechol. The substrate specificity assay suggested that 23DHBD was essential for cleavage of bi-cyclic aromatic compounds during the course of aromatic compound biodegradation in Arthrobacter sp. strain PJ3.     

Reconstruction of linkage maps in the distorted segregation populations of backcross, doubled haploid and recombinant inbred lines

Non-Mendelian segregation of markers, known as distorted segregation, is a common biological phenomenon. Although segregation distortion affects the estimation of map distances and the results of quantitative trait loci (QTL) mapping, the effects of distorted markers are often ignored in the construction of linkage maps and in QTL mapping. Recently, we have developed a multipoint method via a Hidden Markov chain method to reconstruct linkage maps in an F2 population that corrects for bias of map distances between distorted markers. In this article, the method is extended to cover backcross, doubled haploid and recombinant inbred line (RIL) populations. The results from simulated experiments show that: (1) the degree that two linked segregation distortion loci (SDL) affect the estimation of map distances increases as SDL heritability and interval length between adjacent markers increase, whereas sample size has little effect on the bias; (2) two linked SDL result in the underesti- mation of linkage distances for most cases, overestimation for an additive model with opposite additive effects, and unbiased estimation for an epistatic model with negative additive-by-additive effects; (3) the proposed method can obtain the unbiased estimation of linkage distance. This new method was applied to a rice RIL population with severely distorted segregation to reconstruct the linkage maps, and a bootstrap method was used to obtain 95% confidence intervals of map distances. The results from real data analysis further demonstrate the utility of our method, which provides a foundation for the inheritance analysis of quantitative and viability traits.