Real time monitoring of nucleic acids ligation based on molecular beacon

The replication, ligation and repair of nucleic acids are essential life processes that based the survival, pro-creation and evolution of creatures. These interactions involving proteins (enzymes) and nucleic acids have fas-cinated great interest of scientists[1]. The nucleic acids ligation has been revealed that it is catalyzed by ligase and co-enzyme (ATP or NAD)[2—4]. Along with in-depth re-search, the nucleic acids ligation becomes a significant and common tool employed in bioengineerin…     

Nitric oxide produced by cumulus cells stimulates maturation of mouse oocytes

The effect of sodium nitroprusside (SNP), a donor of nitric oxide, on meiotic maturation of mouse oocytes was studied by injecting N^w-nitro-L-arginine methyl ester (L-NAME) intra-peritoneal (ip), a nitric oxide synthase inhibitor, or culturing oocytes in the medium supplemented with L-NAME or hypoxanthine (HX) to arrest the spontaneous oocyte maturation in vitro. The results showed that the inhibitory effect of L-NAME by injecting 10 mg/kg ip on extrusion of the first polar body only could be reversed by injecting 2.5 mg/kg SNP with L-NAME simultaneously (P < 0.05). Half an hour later ten mice died when given 10 mg/kg SNP ip. The treatment of some concentrations of SNP (10^–7, 10^–6, 10^–5mol/L) significantly stimulated meiotic maturation to metaphase Ⅱ stages in cumulus enclosed oocytes in the presence of HX. However, other concentrations of SNP (10^–8, 10^–4, 10^–3 mol/L) had no effect on HX-arrested oocyte meiotic maturation. The optimal concentration of SNP on CEOs had no effect on DOs. The dose of 10^–3 mol/LL-NAME demonstrated a significant suppression in formation of PB₁, but not in GVBD. This inhibition was reversed by the addition of SNP. These results indicated that the physiological levels of NO produced by cumulus cells could stimulate meiotic maturation of mouse oocytes both in vivo and in vitro.     

试论人才成功的主客观条件

论述了送变电施工企业人力资源管理的现状,讨论了送变电施工企业应如何提升自己在行业中的人力资源管理问题。     

利用性诱剂在苹果园控制苹小卷叶蛾的技术研究

文章阐述了利用性诱剂对苹果园控制苹小卷叶蛾的研究及试验方法。     

从2006年诺贝尔奖看当代物理学的发展

瑞典皇家科学院将2006年度诺贝尔物理学奖授予马瑟（J．Mather）和斯穆特（G．Smoot）两位教授，表彰以他们为首的学者利用宇宙背景探测器（Cosmic Background Explorer，缩写为COBE）的数据，证实宇宙微波背景辐射的黑体形式．并发现各向异性。这次诺贝尔奖的颁布，肯定了精确宇宙学的一些重要成果。     

抓好心理健康教育 提高学生心理素质

从抓好学生心理健康教育入手,阐述了提高学生素质的重要性。     

四阶边值问题多重正解的存在性

讨论了含弯矩项u″的四阶边值问题{u(4)(t)=f(t,u(t),u″(t),t∈(0,1)),u(0)=u″(0)=u(1)=u″(1)=0至少存在两个正解.讨论所用的主要工具为锥映射不动点指数定理.     

Holocene climatic and environmental changes recorded in Baahar Nuur Lake core in the Ordos Plateau, Inner Mongolia of China

A 5.3 m lake core was drilled in Baahar Nuur Lake in the Ordos Plateau, and measurements of meangrain size, organic δ 13C (δ 13Corg), organic carbon content (TOC), C/N, carbonate content, carbonate δ 13C(δ 13Ccar) and δ 18O (δ 18Ocar) were conducted for retrieving the Holocene chronosequence of climaticchanges based on 15 AMS 14C dates. The record documented four major stages of climate change inthe Ordos Plateau: (IV) a cold and dry condition before ～7.65 14C ka BP; (III) a warm and humid stagebetween ～7.65 and ～5.40 ka BP; (II) a generally drier and cooler climate since ～5.40 ka BP with twohumid events occurring from ～4.70 to ～4.60 ka BP and from ～4.20 to ～3.70 ka BP, and (I) a dry climatecharacterized by complete desiccation of the lake after 3.70 ka BP. Stage III can be further divided intothree sub-stages: (IIIa) a warm and humid episode from ～7.65 to ～6.70 ka BP, (IIIb) a warm and relativelydry episode from ～6.70 to ～6.20 ka BP, and (IIIc) the magthermal and maghumid episode of the Holo-cene from ～6.20 to ～5.40 ka BP.     

单节锂离子电池保护电路的改进

提出了一种低成本的单节锂电池保护回路系统,采用0.6μm混合信号CMOS工艺和修调技术使芯片具有低功耗、高精度检测电压等特点.通过基准电路和取样电路设计的改进,使保护电路实现了多种保护功能,并且具有很高的检测电压精度.模拟结果表明,该电路在温度为25℃时过充电保护电压的检测精度达到了±25mV,耗电流仅为3.5μA,满足高精度检测电压的要求.     

图书馆管理手段的探讨

论述了图书馆管理中期刊装订的必要性以及期刊流通的必然性,分析了期刊装订与期刊流通的辨证关系,确定了期刊装订与期刊流通在期刊工作中的重要地位.