结核分支杆菌Ag85B DNA疫苗构建、免疫原性及其保护效力

构建结核分支杆菌分泌蛋白Ag85B编码基因的DNA疫苗,研究其免疫原性和保护效力.构建该DNA疫苗并经鉴定后,应用Qiagen试剂盒大量纯化无内毒素的质粒DNA.将C57BL/6小鼠随机分为4组,分别3周间隔3次用生理盐水（A）、载体质粒（B）、卡介苗（C）、Ag85B DNA疫苗（D）免疫小鼠,通过ELISA方法检测血清中抗Ag85B抗体及其亚型.第三次免疫后28 d用结核分支杆菌H37Rv攻击,观察小鼠体重变化,攻击4周后检测细胞因子IFN-γ、IL-12,进行肺组织病理检查及菌落计数等.经Ag85B DNA疫苗免疫的小鼠特异性抗体IgG均显著高于对照组,而且IgG2b均高于IgG1和IgG2a.结核分支杆菌攻击后各组小鼠体重变化呈现不同趋势,A、B组小鼠体重从第3周开始下降;C组体重总体呈上升趋势;D组体重第2周开始下降,之后维持不变.MTT法检测脾淋巴细胞增殖实验中D组的刺激指数显著高于其它组;D组小鼠脾淋巴细胞转化率也显著高于A、B组.肺组织菌落计数C组最低,其次是D组.大体病理结果显示C组和D组肺组织病变较A、B组轻.A、B组肺泡腔内有较多渗出液,肺泡壁充血、水肿.C组肉芽肿数目最多.上述结果表明Ag85B DNA疫苗具有较强的免疫原性,呈TH1型细胞介导的免疫应答.Ag85B DNA疫苗的免疫保护效力不如BCG,有待进一步研究其应用价值.     

人CtBP2与CCNH相互作用的初步研究

CtBP2(E1AC-terminal binding protein 2)作为辅阻遏物与多种转录因子联系而参与到很多生物过程中,如细胞分化、凋亡、发育和肿瘤发生等,然而其中许多作用机制尚不明了.为了对CtBP2进行深入研究,利用高通量酵母双杂交技术,以人CtBP2为诱饵,与含有1000个人肝基因克隆的酵母双杂交文库进行接合筛选获得了一个与它相互作用的猎物蛋白CCNH(Cyclin H).通过GST-pull down、免疫共沉淀和亚细胞共定位等实验进一步证明了这两个蛋白在体外和体内的相互作用.     

CRT显示器色空间变换方法的比较

分别采用多项式拟合、三维查找表和神经网络方法,建立CRT显示器设备颜色空间RGB与输出CIEXYZ色空间的非线性对应关系。完成了设备颜色显示的特征化。对两个常用的显示器进行了实验,并进行色差分析,比较三种方法的精确度。实验结果表明。对常用显示器的特征化,多项式拟合的方法精度较低;曼维查找表和神经网络技术可以满足精度要求,其中神经网络方法最具优势,GIELab平均色差为0．23-3．71。     

电刺激小脑顶核对大鼠心肌梗死后心脏神经再生的影响

探讨预先电刺激小脑顶核(FNS)对大鼠心肌梗死(MI)后心脏神经再生的干预作用。将90只Wistar大鼠随机分为:①MI组,结扎左冠状动脉前降支(LAD);②预先电刺激小脑顶核后再予以LAD结扎组(FNS MI组);③毁损小脑顶核后电刺激该部位,再行LAD结扎组(FNL FNS MI组)。各组又分MI后1,7,21d3个时间点。另设假手术组(Sham组)。应用免疫组织化学方法显示心脏组织胆碱乙酰转移酶(CHAT)和酪氨酸羟化酶(TH)。检测各时间点梗死区与非梗死区TH、CHAT阳性神经纤维分布密度,MI组与Sham组相比显著减少(P<0.01);FNS MI组较MI组显著增多(P<0.05);FNL FNS MI组与MI组比较无显著性差异(P>0.05)。因此,电刺激小脑顶核可增加梗死区和非梗死区心肌组织TH、CHAT染色阳性神经纤维密度,促进MI后心脏神经纤维再生。     

浅析武汉科技大学合并后统一图书分类的工作

阐述了高校图书馆合并后统一图书分类法工作的紧迫性,探讨了统一图书分类法的方案、原则、方法及步骤,分析了在统一图书分类的实际工作中会出现的具体问题,指出《中图法》是分类标引标准化的发展方向.     

中国经济增长的动因分析与政府作用

分析了中国经济快速发展的原因,认为经济增长源于资本、劳动力等要素的不断积累和资源的优化配置,而技术进步和制度的改革与完善则强化了这一过程,在这个过程中,政府起到很大作用。     

Extraction of Signal Frequency in Laser Ranging of Space Target Based on FPGA

Aiming at the problems in a linear frequency modulation continuous wave(LFMCW) ranging lidar system, such as low signal-to-noise in echo beat frequency and difficult extraction of the signal frequency, a method with high-speed and high accuracy based on field programmable gate array(FPGA) is proposed. Firstly, the laser echo beat signal is sampled by high-speed analog to digital converter(ADC) and then processed in FPGA for data preprocessing. After the signal is processed by the 8 192-point FPGA will obtain the frequency spectrum and then apply the frequency spectrum to data processing and adaptive noise signal peak detection. These two steps realize the real-time and accurate extraction process of the target echo beat signal frequency. Finally, the spectrum data is transmitted to the host computer and processed through the external data acquisition board for real-time spectrum display. Experimental results show that data preprocessing and spectrum data processing can effectively suppress DC bias and system modulation noise. Adaptive noise peak detection can accurately divide the threshold contour according to the dynamic noise of the system and realize the detection of target signal peak. When the sampling clock of the system is 100 MHz, the time needed for each calculation of the spectrum is 81.92 μs, and the refresh rate of the spectrum reaches 12.2 kHz, which meets the real-time requirements of the system.     

加快建设节约型社会促进山西经济社会发展

分析了山西建设节约型社会的客观必然性,指出了近期山西建设节约型社会需要抓好的重点工作,提出了山西建设节约型社会的若干建议。     

南水北调中线工程向黄河相机补水政策的探讨

针对黄河流域水资源贫乏的现状,分析了南水北调中线工程向黄河相机补水的必要性和可行性,进而提出了相关的政策建议。     

Will Tax Burden Be a Stumbling Block to Carbon-Emission Reduction?Evidence from OECD Countries

The influence of taxation on the economy and society is everywhere. Given the fact that CO2 emission is closely connected with human activities, the question naturally arises whether the tax burden affects the carbon emissions. To address this issue, based on the data of 21 OECD countries over the period from 1991–2014, we use the ratio of tax revenue to GDP as a proxy of the tax burden and employ a panel quantile model with the non-additive fixed effects for analysis. The results suggest that the impact of the tax burden on carbon emissions is heterogeneous across countries based on the level of carbon emissions. Moreover, we find a stable U-shaped relationship between the tax burden and carbon emissions whether for countries with a high or low level of carbon emissions. Our findings suggest that moderate taxes could help reduce carbon emissions, but the effect of excessive taxation is the opposite.