A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

The objective of this study is to propose a more accurate and faster MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r ²=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125~32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7~8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24~28 h for ADA. The MCA provides an accurate and rapid method for quantification of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.     

广东高校篮球教练员的现状研究

本文运用文献资料法、问卷调查法、数理统计法等研究方法,以广东省十所普通高校篮球教练员为研究对象,分析现状和存在的问题,提出了改善建议.     

构建读书活动网络平台促进高校网络文化健康发展

分析了构建读书活动网络平台的意义,探讨了利用读书活动网络平台开展读书活动的措施。     

移动通信系统安全机制发展的研究

从移动通信系统演进的角度介绍了安全机制的发展历程,分析研究了2G的安全性措施,阐述了3G认证签权方案及其优缺点,并对移动通信安全机制提出了一些见解。     

针剂头孢曲松钠对B-Z化学振荡反应的影响及其分析检测

研究了针剂头孢曲松钠对经典的B-Z化学振荡体系的扰动,结果表明头孢曲松钠的浓度与扰动后振荡周期的改变量存在一定的线性关系,其线性范围为6.15×10-7~5×10-3mol/L,最低检测限为6.25×10-8mol/L.探讨了针剂头孢曲松钠对化学振荡反应扰动的可能机理.     

创造性思维的系统化存在

创造性思维的被激发以及被实施的整个过程可视为特定系统。这个系统从基础逻辑认知开始，经由灵感和直觉等非逻辑思维形式，最终被逻辑思维整合。     

临近空间单粒子效应的数值模型和电路模拟

针对临近空间单粒子效应进行了数值模型仿真和特征尺寸为0.1 μm的反相器电路的脉冲注入模拟研究。数值仿真结果表明器件临界电荷随着工作电压的降低而减小,敏感横截面随着临界电荷的降低而逐渐增大。临近空间微电子器件的单粒子翻转概率随敏感横截面增大而上升,但其又随临近空间高度的增加而下降。此外,利用SPICE软件脉冲注入模拟观察到了反相器电路的单粒子翻转现象。所得结论有助于深入研究临近空间的单粒子效应并为器件抗辐射加固提供了理论依据。     

信号与系统中的matlab程序

在现行的理工科教学中,matlab的应用已经成为一个重要的内容。尤其在电子类的核心专业课信号与系统中,matlab的作用更加值得教师关注。文章就信号与系统这一课程中牵涉到的一些典型matlab程序,进行了注解和说明。试图在信号与系统和matlab的应用两方面找到了一个更为恰当的结合点,使学生和教师在专业知识和应用性工具方面,得到一个更好的处理方式。     

基于循环经济模式的交通运输可持续发展研究

文章阐明了循环经济的基本概念和基本理论，在分析我国交通运输业可持续发展面临的诸多问题后，结合循环经济模式对我国交通运输可持续发展战略进行了研究。     

Multiple MIPS 4Kc cores based interrupt controller design and its implementation on HDTV SoC platform

A multiple MIPS 4Kc processor cores based interrupt processing system is introduced. The interrupt controller plays a key role in the high definition television （HDTV） system-on-a-chip （SoC） platform, especially when it is a multiple processor system. Based on a general introduction to the whole HDTV SoC platform, a layered interrupt controller and its implementation are discussed in detail. The proposed scheme was implemented in our FPGA verification board. The results indicate that our scheme is reliable and efficient. Meanwhile, as a functional intellectual property （IP）, the interrupt controller has reusability and expandability with the layered structure.