NPTⅡ基因在满江红鱼腥藻染色体DNAglnA位点的定点整合及对泌氨和细胞形态结构的影响

本研究构建了含glnA基因片段和TPTⅡ基因的单交换整合型重组质粒pGN1-4,并通过电激法,三亲接合转移和二亲接合转移法号入到满江红鱼腥藻细胞中。用蓝细菌原位杂交法筛选出转化细胞菌落。再经Southern杂交方法进一步证实了转化结果。化学测氨法表明,培养后7～14d泌氨达到高峰,转化细胞泌氨比对照高40％。光镜观察表明,转化细胞藻丝变短,易断成单个细胞,异形胞频率降低;扫描电镜观察指出,转化细胞外壁有鳞片状鞘层,而对照较光滑。     

广义Burger方程的强对称,对称及李代数

本文讨论了广义Burger方程U_1=F~2u+Fu~2(F是与t无关的一阶常系数线性偏微分算子)的强对称和对称,找到了三串对称σ_(mn),∑_(mn)和τ_(mn),并得到了对称所满足的李代数。     

A novel model for extending international co-operation in science and education

In September 1994 the University of Twente, the Netherlands, and Zhejiang University, China, decided to cooperate in the field of science, education and management. After several visits of delegations from both sides it was considered worthwhile to explore further opportunities for mutual cooperation. The directors of international cooperation on each side jointly commissioned a project to investigate the potential in a systematic way and to establish further contacts where appropriate. This paper reports on the results of the research cum matching project. To reveal promising matches between multiple departments of both academic institutions a matching model for universities was designed. The study was carried out along two parallel lines. In the research line the theoretical framework was developed into a model for international university co-operation. Moreover, an analysis was carried out on internal, external and cultural aspects resulting in a set of thirty four influencing factors. In the matching line a total of seventy interviews were held in order to identify promising matches between units at both universities. This line resulted in eleven promising matches for further co-operation. The novel model appeared useful in analyzing the variety of factors and in developing matches between both universities. In the further implementation of the model the issues of "level of co-operation" and "top-down versus bottom-up" need to be addressed in more detail.     

变形母题的文学阐释

中外文学中尤其是神话作品中存在着许多以“变形”母题为表现特征的作品。这些作品有个共同的特点即具备以下两个方面的特定因素：特定的人物即发生“变形”的主人公，特定的情境即导致异化、发生变形的具体情节语境。本文试着从“变形”母题与社会背景演绎，变形母题与异化思想，变形母题与民族意识三个方面对中外文学中的“变形”母题作一定的平行研究，探讨“不同的创作环境、不同的历史阶段给类似的文学主题带来了怎样不同容量的艺术表达方式”。     

非水体系合成蜂窝状金属氧化物介孔材料

以无水金属氯化物为前驱体,CH2=CHCH2O(C2H4O)12H为模板剂,在无水乙醇体系中合成出三维蜂窝状金属氧化物介孔材料.利用XRD、BET、TEM等手段对其进行了表征.经过400℃焙烧除去表面活性剂后,得到高质量的蜂窝状金属氧化物介孔分子筛.热稳定性好、比表面积高、孔壁晶化较好的蜂窝状金属氧化物介孔材料在催化、吸附与分离、传感器等领域有着潜在的应用价值.     

复合材料飞轮内部损伤的实时诊断

材料内部的损伤会引起材料动态性能的变化,通过传感器记录材料内部的振动信号,结合频谱分析和小波分析技术可以辨识材料内部有无损伤、损伤的程度以及类型.依据这种原理,结合复合材料飞轮电池的工作条件,提出了一种在线检测飞轮电池转子损伤的方法,并通过对大量试样的测试,建立了一个识别飞轮损伤的数据库.     

二苯甲酮肟的Beckmann重排反应研究

报道不同路易斯酸性催化剂作用下二苯甲酮肟的Beckmann重排反应的结果，即分别以PCl5、H2S04、HCl-Ac2O、H2SO4-Ac2O、H3PO4-Ac2O、PPA、HCl-Ac2O-AcOH为催化剂进行比较实验．发现H3PO4-Ac2O是二苯甲酮肟进行Beckmann重排反应的理想催化剂之一．     

山西管涔山林区灌丛研究

采用样方法对灌丛进行调查，依据灌丛的优势种对其进行命名．在中山-低山地带的灌丛以沙棘群丛、三裂绣线菊群丛分布最广；在亚高山地带的灌丛以鬼见锦鸡儿群丛和金露梅群丛为主．灌丛的形成受人类活动以及环境条件的影响，在环境条件中水分、温度和风对灌丛的高度、群丛的结构、种类组成均有影响．     

新型压水堆自然循环特性计算分析

针对新型压水堆的结构特点，在一维质量、动量和能量守恒方程的基础上，建立了描述其稳态自然循环特性的数学模型．模型对新型压水堆在稳态自然循环过程中可能出现的流动形式和换热状态进行了充分考虑．通过数值迭代求解该模型，对新型压水堆的自然循环特性进行了理论分析．计算结果表明：反应堆自然循环流量随堆芯功率的增加而增加，并且是堆芯功率的指数函数；在不同蒸汽压力下，反应堆的自然循环流量不同，且随蒸汽压力的增加而增加．冷热芯位差越大，反应堆自然循环能力越大．该研究对我国新型压水堆的概念设计提供了重要的理论依据．     

Purification and relationship with gastric disease of a 130 kDa（CagA） protein of Helicobacter pylorl

Objective:The aims of this research were to purify and identify the 130 kDa(CagA) protein of H.pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa(CagA) pro-tein and gastric diseases.Methods:The procedure for isolating the protein included 6 mol/L guanidine ex-tract,size exclusion chromatography and elusion from gel.Sera of 68 patients with gastric diseases(44 with chronic gastritis,15 with atrophic gastritis,7 with peptic ulcer disease,2 with gastric cancer) were obtained,and the serological response to CagA was studied by Westem-blot using the purified protein,Results;The pu-rified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric point about of 8.1. Among 68 sera,43 sera could recognize the purified protein associated with chronic agastritis 47.7%(21/44),atrophic gastritis 86.7%(13/15),peptic ulcer disease 100%(7/7),gastric cancer 100%(2/2).Compared with each other,the difference was significant(x^2=13.327,P=0.004),and 130 kDa(CagA) protein was associated with severe gastric diseases(rs=0.442,P=0.001).Conclusion:The 130 kDa(CagA) protein was associated wih severe gastric diseases.