医用细菌纤维素的制备及其细胞相容性

以椰汁作为基本的碳源和生长因子来源,通过添加成分明确的乙醇及酵母膏作为补充碳源和氮源,并补加相应的微量元素,获得了成分简单、明确的医用细菌纤维素培养基;该培养基各成分含量相对稳定,提高了细菌纤维素的发酵速率和产量,所获得的产物厚度可满足不同的医用需求.将碱液分别与十二烷基硫酸钠(SDS)、超声波及乙醇联用对发酵产物进行纯化处理.结果表明:碱液与SDS联用时纯化效果最好;纯化所得样品的杂质N含量低于检测限,透光率高,在可见光范围内与活体角膜透光率相近,L929细胞相容性良好.     

5G-TSN系统下的高精度时间同步

第五代移动通信系统(the fifth generation communication system, 5G)与时间敏感网络(time sensitive networking, TSN)集成的5G-TSN系统需要实现高精度的时间同步。为此，提出一种多波束联合的定时同步算法提高同步信号检测成功率，实现高精度5G-TSN时间同步。首先，根据多个波束间的时域关系计算波束间同步信号块的互相关的组合，求出期望作为门限值；然后，利用超过门限值的若干波束进行互相关叠加峰值计算最大峰值对应的时域索引，并完成主同步信号定时同步；最后，利用时频域结合地方式对传播时延进行估计以减少同步误差。仿真结果表明，采用基于多波束联合的定时同步算法，相比传统算法，相关峰值更加显著，能有效地提高定时同步成功率。     

地下钢筋混凝土岔管围岩稳定及结构数值分析

以国内某抽水蓄能电站的钢筋混凝土岔管工程为例,从围岩开挖支护、围岩与衬砌相互作用和钢筋混凝土结构等方提出了围岩、混凝土等材料的数值模拟方法,通过三维有限元线性和非线性计算,分析研究了地下钢筋混凝土岔管在施工、运行等工况下,围岩、衬砌结构的应力与变形,以及混凝土岔管结构配筋和配筋后应力、裂缝开展等问题.结果表明：在开挖阶段由于地应力释放在主、岔洞交叉处会产生拉应力,塑性区开展较深;正常运行工况在内水压力作用下岔管结构大部分区域存在拉应力,裂缝较大;检修工况在外水压力作用下仅在主、岔管交叉处存在拉应力区,裂缝较小;结构配筋时,轴向配筋应该由外水作用的工况来控制,环向配筋由内水作用为主的工况来控制.     

1000 mm2 cross-section wire a led four tension exhibition put to traction practice

文章介绍了宁东一山东±660 kV直流输电示范工程(晋4标段)1 000 mm2大截面导线一牵四张力展放施工中,使用4个转向滑车转向布置牵引场的实践过程及施工中的注意事项.     

小学应用题教学的一般技巧

本文总结了小学应用题教学中的一般规律,只要我们能领会它的规律,遵循小学生思维发展的规律和认知特点,在教学中结合本班学生基础能力的实际情况,灵活变通地开展教学活动,定能培养起学生的学习兴趣,有效地提高学生解答应用题的能力.     

PTC124 targets genetic disorders caused by nonsense mutations

Nonsense mutations promote premature translational termination and cause anywhere from 5-70% of the individual cases of most inherited diseases. Studies on nonsense-mediated cystic fibrosis have indicated that boosting specific protein synthesis from <1% to as little as 5% of normal levels may greatly reduce the severity or eliminate the principal manifestations of disease. To address the need for a drug capable of suppressing premature termination, we identified PTC124-a new chemical entity that selectively induces ribosomal readthrough of premature but not normal termination codons. PTC124 activity, optimized using nonsense-containing reporters, promoted dystrophin production in primary muscle cells from humans and mdx mice expressing dystrophin nonsense alleles, and rescued striated muscle function in mdx mice within 2-8 weeks of drug exposure. PTC124 was well tolerated in animals at plasma exposures substantially in excess of those required for nonsense suppression. The selectivity of PTC124 for premature termination codons, its well characterized activity profile, oral bioavailability and pharmacological properties indicate that this drug may have broad clinical potential for the treatment of a large group of genetic disorders with limited or no therapeutic options.