N-terminal of L protein of vesicular stomatitis virus contains a new signal sequence

The L protein (241kD) of vesicular stomatitis virus (VSV) is the most important snbnnit of the replication complex. The existence of specific localization signal in the L protein was investigated by making recombinant constructs expressing truncated mutants of the L protein fused to green fluorescent protein (GFP) in transient transfection assays. The chimeric genes encoding varied N-terminal of L and GFP gene were put under the control of T7 promoter or CMV promoter. The fusion proteins were transiently expressed in BHK-21, COS-7, CHO or Hep G2 cells. When more than 120 residues were deleted or only 96 residues were kept on the N-terminal, the fusion proteins were shown to be distributed throughout the cells, cytoplasm and nucleus under the confocal microscope. However, other chimeric proteins with 120 or more amino acids were dotted and distributed in the perinuclear regions. And the fusion protein with 96—120 aa has the similar distribution. A thirteen-residue peptide QGYSFLHEVDKEA (108—120) was identified as localization signal, whose function would be absolutely distributed with the deficiency of D or V. Our results show that there is an independent localizing signal in N-terminal domain of L protein of VSV and this functional signal is conserved in different cell lines.     

Contributions of different land cover types in Otindag Sandy Land and Bashang area of Hebei Province to the material source of sand stormy weather in Beijing

Different land cover types in Otindag Sandy Land and Bashang area of Hebei Province are linked to a material source of sand stormy weather in Beijing based on results of field vegetation and soil survey and laboratory works. Results of grain size analysis show that dust release potential in per unit area of moving sandy land is small,while lowland meadow and meadow steppe in stony hills have high potential of releasing dusts in per unit area during sand stormy weather occurrence. Further considering the effects of vegetation, it is inferred that the moving sandy land served as a material source of past dust storm and the possibility of dust release in per unit area is low in current time.Typical steppe in stony hills is undergoing desertification and its dust release possibility in per unit area is high. Farmland has strong potential of release dusts when they are ploughed in spring, but a large amount of therophytes grow and thus prevent dusts from release when cultivation was terminated.Potentials of dust release in per unit area in fixed sand dunes,stony mountain meadow steppe and low land meadow are lowdue to high cover of perennials. Sand dune reactivation and desiccation of lakes and lowlands under estimated future climatic change will make them serve as a future material source of sand stormy weather.     

WASP6系统在三峡库区水质仿真中的应用

以1996—2002年三峡库区干流代表性断面水质监测资料为基础，运用WASP6系统对三峡库区水质进行了模拟与预测．结果表明，模拟值与实测值吻合较好，相对误差在15％以下的占92％；在维持现有污染负荷不变的情况下，蓄水前后水质变化不大，各断面水质均可达到地面水环境质量标准中的Ⅲ类或Ⅱ类水质标准；低水位运行的情况下，各断面的水质明显好于高水位运行情况．     

钢筋混凝土轴拉构件刚度试验研究

通过理论分析与实验研究，提出了钢筋混凝土轴拉构件正常使用时的刚度计算公式，且分析了公式计算值与实测值的符合程度及其影响因素。     

过渡金属表面反应能量学计算系统的开发

利用VB6．0和Access7．0开发了过渡金属表面反应能量学的计算系统．该软件基于UBI-QEP的理论方法．采用模块化设计．其功能模块有3个：(1)数据库模块．利用该模块可以实现气相解离能以及小吸附基团在各种过渡金属表面吸附热的查询、添加与修改；(2)化学吸附热计算模块，利用该模块可以调用数据库中的数据对吸附基团在不同吸附方式的吸附热进行计算；(3)基元反应的活化能垒计算模块．利用该模块可以对基元反应的活化内能进行计算，进而分析其反应的难易与历程．该系统实现了UBI-QEP的理论方法计算的程序化，可用于多相催化反应的催化剂优选和机理探讨．     

试析电化学装置两极机制及命名原则

阐述了电化学装置两极机制及其命名原则，从氧化还原观点阐明了电化学反应的实质；从热力学角度论证了能量转换的特点。     

232规则元胞自动机的稳定性

研究满足三重局部变换规则——232规则的一维有限元胞自动机分别在固定边界条件和周期边界条件下的稳定性，刻划了该类元胞自动机中不动点的个数及它们的瞬时长度．     

基于数学形态学的图像处理方法

从图像的描述方法出发，在二值形态变换的基础上，通过引入图像的阀集合和本影，导出了多值形态学基本形态变换建立的方法．并揭示了二值形态学与多值形态学在结构算法上的相互联系．在图像处理的形态学方法上，提出了基于形态变换的图像边缘提取、多种形态梯度、形态骨架表示方法，以及具有去噪和形态平滑功能的开、闭形态变换在图像分析中的应用．     

用于宽带取样示波器的Nose-to-nose校准技术

介绍一种宽带系统校准的新方法，对比几种传统的校准方法，这种称为nose—to-nose的校准方法是目前校准精度最高的一种．这项技术所基于的一个基本事实是：当示波器的取样电路对直流电压取样时，会产生一个极窄的脉冲到输入端，这个脉冲正比于系统的冲激响应，称为kick-out脉冲，这样将两台示波器的输入端对接就在第二台示波器上得到了含有两台示波器冲激响应的卷积输出，应用反卷积算法可以得到这两台示波器的冲激响应函数．     

AIP吸收器传质特性中试装置及KLa测试方法研究

基于不依赖空气动力装置（AIP）排出CO2气体旋转床吸收器传质特性研究要求，选择S-195型柴油机为AIP排出气体发生器，优化设计了气、水流程，建立了波纹碟片填料旋转床吸收器的传质特性中间试验装置（简称中试装置），该装置具有多参数调节和测试功能。针对在较高气体压力下，AIP排出气体旋转床传质试验无法直接测算其体积传质系数（Kla），提出了一种间接测算Kla实验值的方法。实验结果表明，用间接测算方法求取的Kla实验值与直接测算值相对误差小于10％。