Influence of cyclic nucleotides on protein synthesis in vascular smooth muscle

Summary The incorporation of leucice-¹⁴C into protein in bovine mesenteric arteries was augmented by cyclic GMP (10^–3 M) and decreased by cyclic AMP (10^–3 M). There was no effect of 5 AMP (10^–3 M). The phosphodiesterase inhibiting drugs theophylline (10^–3 M) and papaverine (5×10^–5 g/ml) both decreased the leucine-¹⁴C incorporation.We are indebted to Mrs.Lena Burlin for hear assistance. Finacial support has been provided by the Swedish State Medical Research Council (No. 04X-101X-4498).     

Ecdysone 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.)

Summary An ecdysone 20-hydroxylase enzyme system that converts -ecdysone to 20-hydroxyecdysone was prepared from the midgut of the tobacco hornworm prepupa. This partially purified enzyme is NADPH dependent and is localized in the mitochondrial fraction of the midgut tissue.     

用自定义C函数创建中文立体上弹式菜单

通过BIOS中断调用建立一组自定义C函数，在文本方式下，以任意背、前景色显示以矩形框修饰的窗口和以任意位置、背景、前景、字体、字型显示汉字，从而建立中文立体上弹式菜单，并给出了实现的源程序．     

栲胶脱硫在工业燃用煤气上的应用

叙述了用拷胶脱除煤气中H2S的基本原理，介绍了栲胶脱硫在工业燃用煤气上的应用情况，并指出了需要注意的事项．     

丝状绕花内插元件强化管内换热技术的研究

丝状绕花内插元件强化管内换热技术是一项综合强化换热的新技术，它应用到管式换热器的制造领域，有效地克服了普通管式换热器换热弱的不足，发挥了坚固耐用、适用范围广、操作弹性大的优点，大大提高了管式换热器的传热效率．是我国管式换热器技术研究的最新进展．     

浅论我国地热资源的开发利用

本文论述了地热资源的属性、开发现状及利用前景，并指出了在开发利用地热资源的过程中存在的问题，提出了解决的办法．     

Splitting an Ordering into a Partition to Minimize Diameter

k consisting of k clusters, with k > 2. Bottom-up agglomerative approaches are also commonly used to construct partitions, and we discuss these in terms of worst-case performance for metric data sets. Our main contribution derives from a new restricted partition formulation that requires each cluster to be an interval of a given ordering of the objects being clustered. Dynamic programming can optimally split such an ordering into a partition P^k for a large class of objectives that includes min-diameter. We explore a variety of ordering heuristics and show that our algorithm, when combined with an appropriate ordering heuristic, outperforms traditional algorithms on both random and non-random data sets.     

Effect of hydrogen peroxide on the binding of Merocyanine 540 to human erythrocytes

The fluorescent dye Merocyanine 540 (MC540) is often used as a probe to monitor the molecular packing of phospholipids in the outer leaflet of biomembranes. In a previous study we showed that the increased staining of erythrocytes with a perturbed membrane structure was mainly due to an increase in the fluorescence yield of cell-bound MC540, rather than to an increase of the number of bound molecules. Erythrocytes and ghosts exposed to continuous fluxes of H₂O₂ exhibited pronounced lipid peroxidation. Further, red blood cells subjected to this form of oxidative stress also showed increased staining with MC540. It appeared that this was caused by a strong increase in binding of MC540, together with a slight red shift of the fluorescence emission maximum and a small increase in the fluorescence yield of bound MC540. The changed MC540 binding characteristics were not observed when lipid peroxidation was suppressed by the presence of the antioxidant BHT in the incubation medium. However, open ghosts exposed to H₂O₂ showed no increase of MC540 binding, excluding a direct involvement of lipid peroxidation. Measurement of fluorescence emission spectra and gel filtration studies showed that MC540 can bind to H₂O₂-exposed hemoglobin. Experiments with erythrocytes lysed in hypotonic medium after exposure to H₂O₂ revealed that peroxidation of lipids with H₂O₂ induced a non-specific permeabilization of the plasma membrane to MC540, thereby allowing MC540 to bind to the oxidatively denatured, more hydrophobic hemoglobin. These results indicate that conclusions about packing of phospholipids in the outer leaflet of the membrane based on increased MC540-staining should be drawn with care. Received 27 September 1996; received after revision 5 November 1996; accepted 27 November 1996     

非标准设备驱动程序与微机DOS的接口

阐述了一种与ＤＯＳ接口的非标准设备驱动程序的编程方法，它增加了非标准设备驱动程序的适用性．