Alternative splicing and expression of the insulin-like growth factor （IGF-1） gene in osteoblasts under mechanical stretch

Insulin-like growth factor 1 (IGF-1) promotes osteoblasts differentiation and bone formation, and its expression is induced by mechanical stretch, thus IGF-1 has been considered an effector molecule that links mechanical stimulation and local tissue responses. In this study, a mechanical stretching device was designed to apply physiological level static or cyclic stretching stimulation to osteoblasts. Different isoforms of IGF-1 mRNA were amplified by RT-PCR from the cells using respective primers and these amplified products were sequenced. An iso-form of IGF-1 splicing product was found to be selectively produced by osteoblasts under stretching stimulation. This IGF-1 isoform had identical sequence with the mechano growth factor (MGF) which was originally identified in muscle cells. Regulations of the expression of the liver-type IGF (L.IGF-1) and MGF in osteoblasts under stretch stimulation were further studied using semi-quantitative RT-PCR. Stretch stimulation was found to promot the expression of IGF-1 (L.IGF-1 and MGF), and for both isoforms expression was more effectively stimulated by cyclic stretch than static stretch. MGF was detected only in osteoblasts subjected to mechanical stretch, suggesting MGF was a stretch sensitive growth factor. Expression of MGF peaked earlier than that of L.IGF-1, which was similar to their regulation in muscle and suggested similar roles of MGF and L.IGF-1 in bone as in muscle cells. The functions of MGF and LIGF-1 in osteoblasts shall be established by further experimental studies.     

由DS1820和AT89C2051构成的简单测温装置

介绍了新型单线数字温度计ＤＳ１８２０和ＡＴ８９Ｃ２０５１芯片的应用原理和由它们构成的单线多点温度测量装置。给出了装置的硬件构成和软件框图。说明了此种测温装置具有多点测量、结构简单和体积小的特点。     

HAb18G/CD147-mediated calcium mobilization and hepatoma metastasis require both C-terminal and N-terminal domains

HAb18G/CD147 is a heavily glycosylated protein containing two immunoglobulin superfamily domains. Our previous studies have indicated that overexpression of HAb18G/CD147 enhances metastatic potentials in human hepatoma cells by disrupting the regulation of store-operated Ca²⁺ entry by nitric oxide (NO)/cGMP. In the present study, we investigated the structure-function of HAb18G/CD147 by transfecting truncated HAb18G/CD147 fragments into human 7721 hepatoma cells. The inhibitory effect of HAb18G/CD147 on 8-bromo-cGMP-regulated thapsigargin-induced Ca²⁺ entry was reversed by the expression of either C or N terminus truncated HAb18G/CD147 in T7721C and T7721N cells, respectively. The potential effect of HAb18G/CD147 on metastatic potentials, both adhesion and invasion capacities, of hepatoma cells was abolished in T7721C cells, but not affected in T7721N cells. Release and activation of matrix metalloproteinases (MMPs), MMP-2 and MMP-9, were found to be enhanced by the expression of HAb18G/CD147, and this effect was abolished by both truncations. Thapsigargin significantly enhanced release and activation of MMPs (MMP-2 and MMP-9) in non-transfected 7721 cells, and this effect was negatively regulated by SNAP. However, no effects of thapsigargin or SNAP were observed in T7721 cells, and expression of HAb18G/CD147 enhanced secretion and activation of MMPs at a stable and high level. Taken together, these results suggest that both ectodomain and intracellular domains of HAb18G/CD147 are required to mediate the effect of HAb18G/CD147 on the secretion and activation of MMPs and metastasis-related processes in human hepatoma cells by disrupting the regulation of NO/cGMP-sensitive intracellular Ca²⁺ mobilization although each domain may play different roles.Received 1 April 2004; received after revision 15 June 2004; accepted 22 June 2004     

亚洲玉米螟性信息素的扩大合成及生测效果

本文进行了亚洲玉米螟性信息素的扩大合成研究,使合成的诸条件进一步完善,确定了最佳碱浓度,为小批量制备提供了可靠数据。田间生测实验的结果表明,所合成的药剂每亩仅用2.2mg即具有明显的防治亚洲玉米螟的效果。     

多值逻辑函数的分解及T门实现

本文首先讨论了多值逻辑函数的分解方法,这种分解方法可以利用真值表的分解较方便地实现。然后给出了利用 T 门实现多值逻辑函数的一种方法。应用这种方法,分别研究了完全指定的函数和不完全指定的函数的分解及 T 门实现。     

金属型非合金化奥-贝球铁性能影响因素初探

初步讨论了影响金属型非合金化奥-贝球铁性能的主要工艺因素——含锰量、奥氏体化时间和等温时间。在试验条件下,含锰量为0.56％,奥氏体化时间60分钟和等温时间90分钟时的综合性能最佳,φ20试棒加工的试样σ_b=1066N/mm~2,δ=3.26％。预计进一步延长等温时间将有利于性能的提高.     

萃取浮选光度法测定微量钼的研究及应用

研究了用甲苯萃取浮选Mo(Ⅴ)—SCN~-—Kφ三元络合物的各种条件。在硫酸介质中,紫红色络合物最大吸收波长位于585nm,摩尔吸光系数为2.8×10~5l·mol~(-1)·cm~(-1),钼量在0—10μg/25ml范围内符合比耳定律。大量常见元素均不干扰,40倍的钨(Ⅵ)亦不干扰钼的测定,方法已应用于钨矿中微量钼的测定,其结果令人满意。     

地方高校“播音与主持艺术专业热”透视

新世纪以来,伴随着“艺考”的升温,中国高等教育出现了前所未有的“播音与主持艺术专业热”.具体表现在开设高校增多、报考学生数量庞大、专业规模迅速膨胀和专业培训红火等方面.专业门槛相对较低、专业收费相对较高、传媒业的迅猛发展、播音与主持人的明星效应等是导致“播音与主持艺术专业热”的主要原因.只有政府、学校、家长、学生共同面对,才能使“播音与主持艺术专业热”降温,从而回归正常轨道.     

FP_tree算法在入侵检测系统中的应用

指出了入侵检测系统中的常见约束条件,即关键属性约束、挖掘范围约束、个数约束.在分析这些约束条件性质的基础上提出了3种剪枝策略,对基于"支持度-可信度"度量机制的FP_tree算法进行了扩展.实验结果表明:新的算法提高了挖掘效率,删除了许多无意义的模式.