室内粉刷石膏抹面施工方法

粉刷石膏是一种具有呼吸功能好、防火性好及绿色环保的抹面材料,在现在的许多工程实践中应用广泛.文章就粉刷石膏的特点、施工工法及施工注意事项进行了介绍.     

阳泉市省道214阳泉过境公路李家庄河防洪评价研究

文章以双拱涵洞穿越省道214阳泉过境公路防洪影响评价为例,对涵洞的过水能力作了系统分析和探讨,论述了改建后的涵洞对50年一遇的洪水能顺利通过,并给出了防治措施。     

金刚砂楼地面的施工方法与质量控制

某大型仓库工程设计采用金刚砂楼地面,为确保施工质量,施工时需认真做好各项准备工作,严格根据气候、温度、砂浆配合比等因素控制好金刚砂撒布的时间.     

Oxysterols direct immune cell migration via EBI2

Epstein-Barr virus-induced gene 2 (EBI2, also known as GPR183) is a G-protein-coupled receptor that is required for humoral immune responses; polymorphisms in the receptor have been associated with inflammatory autoimmune diseases. The natural ligand for EBI2 has been unknown. Here we describe the identification of 7α,25-dihydroxycholesterol (also called 7α,25-OHC or 5-cholesten-3β,7α,25-triol) as a potent and selective agonist of EBI2. Functional activation of human EBI2 by 7α,25-OHC and closely related oxysterols was verified by monitoring second messenger readouts and saturable, high-affinity radioligand binding. Furthermore, we find that 7α,25-OHC and closely related oxysterols act as chemoattractants for immune cells expressing EBI2 by directing cell migration in vitro and in vivo. A critical enzyme required for the generation of 7α,25-OHC is cholesterol 25-hydroxylase (CH25H). Similar to EBI2 receptor knockout mice, mice deficient in CH25H fail to position activated B cells within the spleen to the outer follicle and mount a reduced plasma cell response after an immune challenge. This demonstrates that CH25H generates EBI2 biological activity in vivo and indicates that the EBI2-oxysterol signalling pathway has an important role in the adaptive immune response.     

S-nitrosylation of NADPH oxidase regulates cell death in plant immunity

Changes in redox status are a conspicuous feature of immune responses in a variety of eukaryotes, but the associated signalling mechanisms are not well understood. In plants, attempted microbial infection triggers the rapid synthesis of nitric oxide and a parallel accumulation of reactive oxygen intermediates, the latter generated by NADPH oxidases related to those responsible for the pathogen-activated respiratory burst in phagocytes. Both nitric oxide and reactive oxygen intermediates have been implicated in controlling the hypersensitive response, a programmed execution of plant cells at sites of attempted infection. However, the molecular mechanisms that underpin their function and coordinate their synthesis are unknown. Here we show genetic evidence that increases in cysteine thiols modified using nitric oxide, termed S-nitrosothiols, facilitate the hypersensitive response in the absence of the cell death agonist salicylic acid and the synthesis of reactive oxygen intermediates. Surprisingly, when concentrations of S-nitrosothiols were high, nitric oxide function also governed a negative feedback loop limiting the hypersensitive response, mediated by S-nitrosylation of the NADPH oxidase, AtRBOHD, at Cys 890, abolishing its ability to synthesize reactive oxygen intermediates. Accordingly, mutation of Cys 890 compromised S-nitrosothiol-mediated control of AtRBOHD activity, perturbing the magnitude of cell death development. This cysteine is evolutionarily conserved and specifically S-nitrosylated in both human and fly NADPH oxidase, suggesting that this mechanism may govern immune responses in both plants and animals.     

一类恒化器模型解的稳定性

讨论一类含1个食饵和1个捕食者种群的恒化器模型的整体解.分析该模型解的一致有界性,运用线性化方法和Lyapunov函数方法研究该模型非负平衡点的局部渐近稳定性和全局渐近稳定性.     

神经电活动稳态调控抑制性突触传递的分子机制

发育中的神经网络需要兼顾生长与稳定这两种相辅相成的需求.稳态可塑性可通过调节兴奋性或抑制性突触传递从而维持神经网络的稳定.已报道的关于稳态可塑性机制方面的研究主要集中在其对兴奋性突触传递的影响,很少关注其对抑制性突触的调控.运用体外培养的原代海马神经元,本项研究发现了持续增强神经元电活动4小时能够诱导抑制性突触传递的稳...     

Converting nonsense codons into sense codons by targeted pseudouridylation

All three translation termination codons, or nonsense codons, contain a uridine residue at the first position of the codon. Here, we demonstrate that pseudouridylation (conversion of uridine into pseudouridine (Ψ), ref. 4) of nonsense codons suppresses translation termination both in vitro and in vivo. In vivo targeting of nonsense codons is accomplished by the expression of an H/ACA RNA capable of directing the isomerization of uridine to Ψ within the nonsense codon. Thus, targeted pseudouridylation represents a novel approach for promoting nonsense suppression in vivo. Remarkably, we also show that pseudouridylated nonsense codons code for amino acids with similar properties. Specifically, ΨAA and ΨAG code for serine and threonine, whereas ΨGA codes for tyrosine and phenylalanine, thus suggesting a new mode of decoding. Our results also suggest that RNA modification, as a naturally occurring mechanism, may offer a new way to expand the genetic code.     

秦岭地区自然保护区的生态旅游发展对策

通过分析秦岭地区自然保护区的类型、特点及发展现状,指出秦岭自然保护区在开展生态旅游时应该通过科学规划、严格管理、加强宣传等手段,达到生物多样性保护和经济发展的双赢。     

基于Beta过程的高分辨ISAR成像

对于高信噪比、完整回波、目标平稳运动等理想观测环境,现有成像技术已经较为成熟,可以获得聚焦良好的高分辨逆合成孔径雷达(inverse synthetic aperture radar, ISAR)像。但在实际中的方位回波缺损与低信噪比观测情况下,随机相位误差等因素会降低现有成像算法的性能甚至使其失效。本文首先建立了ISAR稀疏观测模型,并基于稀疏贝叶斯学习理论,通过引入Beta过程非参数先验构建层级概率模型,进而交替利用Gibbs采样及最大似然方法对ISAR像及随机相位误差进行估计。实验结果表明,所提方法在低信噪比、回波缺损等复杂观测环境下能够获得聚焦良好的ISAR图像。