尿素、氯化铵、碳酸铵对牛奶样品微量凯氏定氮法的干扰

用微量凯氏定氮法测定未加含氮干扰物质的牛奶蛋白含量,再用微量凯氏定氮法测定加尿素、氯化铵、碳酸铵的牛奶蛋白含量,然后对添加含氮干扰物质前后牛奶蛋白含量进行比较.测定未加含氮干扰物质的牛奶蛋白含量时,微量凯氏定氮法有较好的重复性和精确性,测定加尿素、氯化铵、碳酸铵的牛奶蛋白含量时,微量凯氏定氮法的重复性和精确性很差.在加入干扰物质前后微量凯氏定氮法测定牛奶蛋白含量的相对误差几乎都大于5%.尿素、氯化铵、碳酸铵非蛋白含氮物质对微量凯氏定氮法测定牛奶蛋白含量具有明显的干扰作用,尿素、氯化铵、碳酸铵非蛋白氮含量越多,对微量凯氏定氮法测定牛蛋白含量干扰越明显.     

海南县域经济发展水平的空间差异与扶贫开发

自改革开放以来,海南社会经济获得了很大的发展,但是县域经济发展水平的空间差异仍十分显著.为了更好地了解海南县域经济发展水平的空间差异,选取人均GDP、城镇化水平、人均财政收入、农民人均纯收入、非农产值、文盲率等7项指标,采用量图分析法,将全省18个市县行政区分为先进、中等、落后3种类型.确定今后扶贫的重点市县.让真正需要扶贫支持的市县得到必要的扶贫支持.尽快的脱离贫困.     

小波分析在水文序列趋势分析中的应用

根据水文时间序列的频率分布特性 ,运用小波分析将不同频率成分组成时间序列按尺度分解成低频和高频成分 ,然后依据小波系数的重构原理还原时间序列的趋势成分 ,从而可以判断水文时间序列的趋势变化 .以黄河上中下游四个水文站的实测年径流序列为例进行了研究 ,研究结果表明多分辨分析用于水文时间序列趋势分析是合理可行的     

A Meta‐learning Framework for Bankruptcy Prediction

The implication of corporate bankruptcy prediction is important to financial institutions when making lending decisions. In related studies, many bankruptcy prediction models have been developed based on some machine‐learning techniques. This paper presents a meta‐learning framework, which is composed of two‐level classifiers for bankruptcy prediction. The first‐level multiple classifiers perform the data reduction task by filtering out unrepresentative training data. Then, the outputs of the first‐level classifiers are utilized to create the second‐level single (meta) classifier. The experiments are based on five related datasets and the results show that the proposed meta‐learning framework provides higher prediction accuracy rates and lower type I/II errors when compared with the stacked generalization classifier and other three widely developed baselines, such as neural networks, decision trees, and logistic regression. Copyright © 2011 John Wiley & Sons, Ltd.     

企业社会责任感知对员工组织公民行为作用机制研究

通过对182名企业员工的问卷调查,实证研究企业社会责任感知对员工组织公民行为的影响,并从社会认同理论和工作嵌入视角探讨了组织认同的中介作用以及工作嵌入的调节效应.结果表明:企业社会责任感知对员工组织公民行为具有正向预测作用;工作嵌入程度越高,组织认同与组织公民行为的正相关越强.文章最后总结了研究结果的理论和实践意义,并指出了未来的研究方向.     

A trapper keeper for TRAPP, its structures and functions

During biosynthesis many membrane and secreted proteins are transported from the endoplasmic reticulum, through the Golgi and on to the plasma membrane in small transport vesicles. These transport vesicles have to undergo budding, movement, tethering, docking, and fusion at each organelle of the biosynthetic pathway. The transport protein particle (TRAPP) complex was initially identified as the tethering factor for endoplasmic reticulum (ER)—derived COPII vesicles, but the functions of TRAPP may extend to other areas of biology. Three forms of TRAPP complexes have been discovered to date, and recent advances in research have provided new insights on the structures and functions of TRAPP. Here we provide a comprehensive review of the recent findings in TRAPP biology.     

Platelet-derived growth factor receptor beta identifies mesenchymal stem cells with enhanced engraftment to tissue injury and pro-angiogenic property

Mesenchymal stem cells (MSCs) are heterogeneous likely consisting of subpopulations with various therapeutic potentials. Here we attempted to acquire a subset of MSCs with enhanced effect in wound healing. We found that human placental MSCs expressing platelet-derived growth factor (PDGF) receptor (PDGFR)-β exhibited greater proliferation rates and generated more colony-forming unit-fibroblast (CFU-F), compared to PDGFR-β^? MSCs. Notably, PDGFR-β⁺ MSCs expressed higher levels of pro-angiogenic factors such as Ang1, Ang2, VEGF, bFGF and PDGF. When 10⁶ GFP-expressing MSCs were topically applied into excisional wounds in mice, PDGFR-β⁺ MSCs actively incorporated into the wound tissue, resulting in enhanced engraftment (3.92 ± 0.31 × 10⁵ remained in wound by 7 days) and accelerated wound closure; meanwhile, PDGFR-β^? MSCs tended to remain on the top of the wound bed with significantly fewer cells (2.46 ± 0.26 × 10⁵) engrafted into the wound, suggesting enhanced chemotactic migration and engraftment of PDGFR-β⁺ MSCs into the wound. Real-Time PCR and immunostain analyses revealed that the expression of PDGF-B was upregulated after wounding; transwell migration assay showed that PDGFR-β⁺ MSCs migrated eightfold more than PDGFR-β^? MSCs toward PDGF-BB. Intriguingly, PDGFR-β⁺ MSC-treated wounds showed significantly enhanced angiogenesis compared to PDGFR-β^? MSC- or vehicle-treated wounds. Thus, our results indicate that PDGFR-β identifies a subset of MSCs with enhanced chemotactic migration to wound injury and effect in promoting angiogenesis and wound healing, implying a greater therapeutic potential for certain diseases.     

2021年新冠病毒变异、跨种传播及疫苗和药物研究热点回眸

概括了新冠病毒主要变异毒株的分类、时空分布和流行动态,以及关键变异位点和其对病毒生物学特性的影响;阐述了2021年新冠病毒基因进化起源研究进展和新冠病毒在人和动物间跨种传播的风险;论述了2021年新冠疫苗和药物的研发和使用现状,以及不完全免疫保护形势下病毒可能的变异动向;提出了疫情防控、病毒流行监测和疫苗药物研发的相应...     

中国关闭/废弃矿井的分类利用与等级评价

 针对关闭/废弃矿井分类利用和等级评价,使用Adaboost迭代算法,建立了一个关闭/废弃矿井利用的可能性等级模型,总结得出关闭/废弃矿井分类利用可分为：关闭/废弃矿井生态文明开发,关闭/废弃矿井地下空间开发利用,关闭/废弃矿井遗留媒体资源开发利用,关闭/废弃矿井地下水资源开发利用,关闭/废弃矿井气油水光互补能源开发利用。确立了20项分类指标进行关闭/废弃矿井等级评价,初步构建出国内关闭/废弃矿井资源数据库,提出关闭/废弃矿井智能精准开发科学构想,构建了关闭/废弃矿井绿色资源评价技术体系,推动关闭/废弃矿井精准智能开采以及城市转型发展。     

2021年滨水空间生态修复研究与规划建设热点回眸

城市更新、韧性城市建设和水生态系统保护与修复是2021年中国生态文明建设的重要议题,滨水空间作为城乡"三生"协同发展的重要空间要素,对城乡建设的品质、环境、生态价值发挥着不可替代的作用,同时滨水空间的城市空间、景观建设、水生态修复与"双碳"目标紧密相关互相促进.回顾了2021年滨水空间生态修复研究、规划建设、运营管理、...