利用脊线检测实现视网膜图像血管中心线的精确提取

提出一种精确有效的基于脊线检测的视网膜图像血管中心线提取算法,该算法首先对原始图像进行脊线检测得到候选视网膜图像血管中心线,然后对原始图像进行照度均衡和多尺度形态学增强处理,最后对各个尺度增强图像在视场ROI区域内进行Otsu双阈值分割或单阈值分割,并将各个尺度的分割结果求和再与脊线检测结果算术相与得到最终的血管中心线。通过对25张荧光造影视网膜图像以及部分彩色视网膜图像进行测试和分析,该算法不但能够检测出低对比度血管和微小血管的中心线,而且提取的血管中心线整体连续性好。将文中算法的结果与血管手动分割血管的细化结果进行比对分析,中心线吻合率平均达到83.5%,且算法性能优于Hoover算法以及保守专家手动检测结果。     

排列中相邻两项的最大公因子

1983年,Erds P,Freud R和Hegyvári N证明了对所有正整数的任一排列a1,a2,a3,…,有liminfi(ai,ai+1)/i≤61/90.本文将结果改进为liminfi(ai,ai+1)/i≤13/20.     

Evaluating the reliability of microsatellite genotyping from low-quality DNA templates with a polynomial distribution model

Molecular studies using trace DNA, such as from museum specimens, ancient or forensic samples and samples obtained noninvasively, often have a common problem of low quality of DNA templates. Amplification errors, such as allelic dropout and false allele, may arise during polymerase chain reaction (PCR) using such samples. A mathematical model which treats homozygotes and heterozygotes discriminately has been developed to measure sample quality and compute the confidence level of using multipletube approaches. We use plucked hair samples collected from 26 individual Sichuan snub-nosed monkeys (Rhinopithecus roxel- lana) to test the model. In this case, a confidence level of 99% can be achieved by three positive PCRs. If the sample quality is very poor and requires many PCR replicates, an alternative multiplestep genotyping method is recommended. This model enables researchers to optimize experimental protocols through pilot studies and obtain reliable genetic information using noninvasive sampling method.     

黑龙江七星河国家级湿地自然保护区生态旅游开发的SWOT分析

采用SWOT分析法较为详细地分析了七星河国家级湿地自然保护区发展生态旅游业的优势、弱势、机遇和威胁。得出黑龙江七星国家级河湿地自然保护区不仅具备景观资源独特、动植物资源丰富、旅游环境优越等开展湿地生态旅游的先决条件,而且拥有国内外生态旅游发展的有利形势和地方政策大力支持的大好机遇;但同时存在湿地保护和专业人员匮乏、旅游...     

Structural insight into brassinosteroid perception by BRI1

Brassinosteroids are essential phytohormones that have crucial roles in plant growth and development. Perception of brassinosteroids requires an active complex of BRASSINOSTEROID-INSENSITIVE 1 (BRI1) and BRI1-ASSOCIATED KINASE 1 (BAK1). Recognized by the extracellular leucine-rich repeat (LRR) domain of BRI1, brassinosteroids induce a phosphorylation-mediated cascade to regulate gene expression. Here we present the crystal structures of BRI1(LRR) in free and brassinolide-bound forms. BRI1(LRR) exists as a monomer in crystals and solution independent of brassinolide. It comprises a helical solenoid structure that accommodates a separate insertion domain at its concave surface. Sandwiched between them, brassinolide binds to a hydrophobicity-dominating surface groove on BRI1(LRR). Brassinolide recognition by BRI1(LRR) is through an induced-fit mechanism involving stabilization of two interdomain loops that creates a pronounced non-polar surface groove for the hormone binding. Together, our results define the molecular mechanisms by which BRI1 recognizes brassinosteroids and provide insight into brassinosteroid-induced BRI1 activation.     

统计流形在切丛上提升的等积仿射结构

具有等积仿射结构的统计流形在贝叶斯统计理论中有着重要应用,主要讨论统计流形及其切丛上的等积仿射结构,得到了统计流形的无挠仿射联络和其在切丛上提升的仿射联络的等积仿射性是一致的.     

一类带调和势的随机非线性Schrdinger方程的爆破解(英文)

讨论一类带调和势的随机非线性Schrdinger方程.众所周知,带白噪声的非线性Schrdinger方程描述了非线形色散波在非齐次或随机介质中的传播.首先给出带调和势的随机非线性Schrdinger方程的一些准备知识,通过建立该方程的性质,运用随机分析方法,证明了临界和超临界情形下解在对应能量空间中的爆破性质,推广了带调和势的非线性Schrdinger方程在确定情形下的相关结果.     

太湖溶藻细菌的分离及评价

从放置于太湖梅梁湾流域的除藻中试装置的人工介质上分离到一株溶藻细菌,经生理、生化和分子生物学鉴定,该株细菌属于芽孢杆菌属.经测定该菌具有较强的溶解铜绿微囊藻和降解微囊藻毒素LR(MC-LR)的功能:该菌(105个/mL)对铜绿微囊藻液(4.5×107个/mL)作用第16,32,48h的溶藻率分别为48%、63%和96%;对MC-LR(2.649μg/L)作用第18,36,54和72h藻毒素降解率分别为15%、29%、73%和100%.应用新建立的实时荧光定量PCR法(RTQ-PCR)对人工介质上的该溶藻芽孢杆菌丰度进行定量检测,结果显示,人工介质上该细菌的丰度约为1%,在7,8,9三个月中呈现递增趋势,其分布具有一定的时空变化规律.     

氧化铁纳米颗粒固定化溶藻菌的除藻作用

为了探讨氧化铁纳米颗粒固定化溶藻菌后对铜绿微囊藻的溶藻作用和降解微囊藻毒素LR(MC-LR)的作用,从太湖水体中分离获得一株属于芽孢杆菌属的溶藻菌,将其与氧化铁纳米颗粒固定化后,以普通Fe2O3作为实验对照,测定其溶藻作用和降解MC-LR作用.以激光粒度仪测定氧化铁纳米颗粒与溶藻菌两者单独和同时存在时的粒径大小及粒径分布,以获得氧化铁纳米颗粒固定化溶藻菌的证据.分析结果表明:在太湖水体中分离获得的一株芽孢杆菌具有较强的溶藻和降解MC-LR的作用,作用24 h后的溶藻率和MC-LR降解率分别达到44.3%和20.5%.同时加入氧化铁纳米颗粒后,溶藻率达到53.0%,MC-LR的降解率达到33.0%.激光粒度仪检测发现,氧化铁纳米颗粒与细菌混合后的颗粒物的平均直径增加到氧化铁纳米颗粒的12.3倍,同时氧化铁纳米颗粒和细菌单独存在时的粒径分布峰消失.氧化铁纳米颗粒是一种具有良好性能的生物固定化材料,可能通过其特有的途径有效地固定化溶藻菌,并增强溶藻菌的溶藻和降解藻毒素作用.     

石杉碱甲片联合丁苯酞软胶囊对轻度阿尔茨海默病患者认知功能的疗效观察

目的 评价石杉碱甲片联合丁苯酞软胶囊对阿尔茨海默病患者认知功能的临床疗效.方法 将100例轻度阿尔茨海默病(Alzheimer's disease,AD)患者随机分为石杉碱甲片联合丁苯酞软胶囊组(治疗组)及单独应用石杉碱甲组(对照组).治疗前及治疗90 d后分别评价两组患者简易智力状态检查(Mini-mental St...