正交试验优选木通中木通酸和木通皂苷Stc的提取工艺

研究木通中2个共有成分木通酸和木通皂苷Stc的提取工艺.采用超声提取,以溶剂种类、提取时间、溶剂用量为影响因素,各取3个水平,采用L9(34)正交表,以木通酸和木通皂苷Stc为考察指标,利用反相高效液相色谱法测定其含量,并对检测结果进行直观分析和方差分析.经正交试验优选的提取工艺:以体积分数为80%的乙醇作溶剂,采用超声提取1.5 h,溶剂用量为药材的12.5倍.对优选的提取工艺进行方法学考察,各项考察结果均符合要求.该提取工艺切实可行,为进一步对不同来源木通药材中木通酸和木通皂苷Stc的含量测定奠定理论基础.     

基于演化博弈的民航旅客群体性事件处置策略

为研究民航旅客群体性事件处置策略,通过演化博弈的相关理论,对各航空公司之间的博弈过程进行分析,从而建立航空公司处置策略模型.并在此基础之上,以某机场航站楼为仿真对象,运用仿真软件来模拟航空公司不同处置策略的作用效果.仿真结果表明,随着延误时间的增加,以及之前干预措施等因素的影响,航空公司工作人员的干预时机及次数均会影响...     

De Casteljau Algorithm and Degree Elevation of Toric Surface Patches

De Casteljau algorithm and degree elevation of Bézier and NURBS curves/surfaces are two important techniques in computer aided geometric design. This paper presents the de Casteljau algorithm and degree elevation of toric surface patches, which include tensor product and triangular rational Bézier surfaces as special cases. Some representative examples of toric surface patches with common shapes are illustrated to verify these two algorithms. Moreover, the authors also apply the degree elevation of toric surface patches to isogeometric analysis. And two more examples show the effectiveness of proposed method.     

石灰砂桩在软土地基处理中的应用

通过实例对石灰砂桩的施工工艺进行了介绍,论述了施工现场的准备工作及施工工序,分析了石灰砂桩施工的优点。     

浅谈小户型住宅

小户型住宅是近年来房地产市场上的热点,通过分析小户型受欢迎的原因,指出小户型将作为我国住宅市场上的一个组成部分长期存在,并在分析现有问题的基础上对其今后的发展提出了建议。     

行政自由裁量权之程序控制

行政自由裁量权与行政执法密不可分。合法、合理地运用行政自由裁量权是建设法治国家的根本保证。从程序层面来探究如何合理规制行政自由裁量权．对进行法治国家的建设有重要的实践意义。     

网络环境下图书馆数字参考咨询服务的模式与特点

文章对目前网络环境下数字参考咨询服务的几种主要模式与特点,进行了阐述与探讨。     

本科西方经济学教学方法的研究与探讨

西方经济学课程是我国高校经济和管理类专业开设的一门重要基础理论课,但本科西方经济学课程教学目前基本上仍然沿用口头讲述法,这不符合本科实际,不利于学生学习。本文分析学习西方经济学的必要性的基础上,提出新的教学方法和相应的教学改革措施。     

Features of the EAP events on the medium-range evolution process and the mid- and high-latitude Rossby wave activities during the Meiyu period

In this paper, features for the evolution of the East Asia/Pacific （EAP） events and their association with high- and mid-latitude Rossby waves during the Meiyu period are analyzed on the medium-range time scale, it is shown that life cycles of the positive and negative EAP events cannot be simply regarded as ＂mirror＂ each other. In the upper troposphere, downward propagations of Rossby wave packets both over high- and mid-latitude regions of Eurasian continent and over the Asian jet region are responsible for generating basic patterns of high- and mid-latitude anomaly centers of the events. In this layer, Rossby wave packets also propagate from the mid-latitude anomaly center to the high-latitude one. In the middle and lower troposphere, the formation of the subtropical anomaly center of the event is mainly attributed to the anomalous convective activity in the tropical Pacific warm pool. The northward Rossby wave energy dispersion from this center is favorable to the enhancement and maintenance of the mid-latitude anomaly center in the same layer. Finally, it might be hypothesized that typical features of the positive and negative EAP events in their mature phase result from the interaction between （or phase-locking of） respective anomalous circulations induced both by quasi-zonal Rossby wave packets embedded in upper troposphere westerly and by quasi-meridional Rossby wave packets in the background flow of the East Asian summer monsoon in the middle and lower troposphere.     

Biosynthesis of selenosubtilisin: A novel way to target selenium into the active site of subtilisin

Glutathione peroxidase （GPx, EC1.11.1.9）, an important anti-oxidative selenoenzyme, can catalyze the reduction of harmful hydroperoxides with concomitant glutathione, thereby protecting cells and other biological issues against oxidative damage. It captures considerable interest in redesign of its function for either the mechanism study or the pharmacological development as an antioxidant. In order to develop a general strategy for specifically targeting and operating selenium in active sites of enzymes, the catalytically essential residue selenocysteine （Sec） was first successfully bioincorporated into the catalytic center of subtilisin by using an auxotrophic expression system. The studies of the catalytic activity and the steady-state kinetics demonstrated that selenosubtilisin is an excellent GPx-like biocatalyst. In comparison with the chemically modified method, biosynthesis exhibits obvious advantages： Sec could be site-directly incorporated into active sites of enzymes to overcome the non-specificity generated by chemical modification. This study provides an important strategy for specifically targeting and operating selenium in the active site of an enzyme.