肥胖以及有氧耐力运动对大鼠血清抗氧化酶和一氧化氮的影响

研究了肥胖以及耐力性运动对大鼠血清抗氧化酶和一氧化氮水平的影响,结果表明,高脂饮食组实验大鼠血清中SOD和CAT浓度显著性下降,MDA明显增加,NO活性下降;高脂饮食结合有氧耐力运动组大鼠血清中SOD和CAT活性以及MDA明显上升,血清中NO水平恢复明显.肥胖大鼠体重增长被明显抑制.结合生化试验结果,确定高脂饮食诱发肥胖后可造成实验动物血清中抗氧化酶活性下降,自由基生成增加,血管舒张功能下降,系统的有氧耐力运动可以有效的逆转高脂饮食造成的自由基增加,NO恢复至正常水平.     

三种方法测定碱性磷酸酶对肝疾病的诊断价值

目的研究血清碱性磷酸酶检测临床肝疾病时的方法学,提高临床诊断价值。方法收集临床不同肝疾病病人的血清,分别用3种方法测定其碱性磷酸酶的活性,并与健康对照组比较作诊断性能分析。结果各肝疾病组血清碱性磷酸酶的活性与对照组相比有极显著差异（P〈0．001）;3种方法分别测定各组之闻的血清碱性磷酸酶活性无显著性差异（P〉0．05）。2．乙基氨基乙醇、2-氨基-2-甲基-1-丙醇、二乙醇胺3种缓冲液测定试剂盒方法测定的灵敏度分别为55．20％、47．96％、51．13％,特异度分别为100％、100％、93．75％。结论血清碱性磷酸酶可以作为各类肝病的临床诊断指标;对肝疾病血清碱性磷酸酶测定用2,乙基氨基乙醇缓冲液的试剂方法测定效果较好。     

三峡水库二期蓄水初期螺山站水位流量变化响应

三峡工程二期蓄水对坝下游江段水情的变化产生了一定的影响.在水库蓄水的初期阶段,分析螺山江段水位流量的变化响应,采用综合落差指数法描述了该河段水位流量关系的变化,并通过同流量水位法初步撂悉了访河段近几年河床冲淤变化情况.结果表明,水库蓄水后.枯水期该站月径流量占径流量的百分比上升了0.43%,丰水期和平水期分别下降了0.2%和0.23%;从该站水位距平值来看,枯水期有所上升,丰水期和平水期均有所下降.时该站水位流量关系进行单值化处理后,发现蓄水后水位流量关系曲线较蓄水前偏左.同时,同流量下水位的变化也反映了该河段泥沙冲淤情况,对此本文作了初步探悉,发琨在同一江段的不同时段都有冲淤的变化.     

Galectin-3 stabilizes heterogeneous nuclear ribonucleoprotein Q to maintain proliferation of human colon cancer cells

Comparative analysis of proteomes using 5-fluorouracil (5-FU)-resistant human colon cancer cell line revealed that decreased galectin-3 expression was significantly associated with retarded proliferation. However, in the presence of 5-FU proliferation rate of cells with suppressed galectin-3 expression did not differ from that of cells with normal galectin-3 expression, even galectin-3 suppression augmented apoptosis. Mechanism by which galectin-3 regulates cancer cell proliferation has been identified in immunoprecipitates of the anti-galectin-3 antibody. Heterogeneous nuclear ribonucleoprotein Q (hnRNP Q) was identified as a protein interacting with galectin-3. Interestingly, while galectin-3 protein was not affected by the hnRNP Q level, its suppression was accompanied by a decrease in hnRNP Q expression. The present study demonstrates that galectin-3 stabilizes hnRNP Q via complex formation, and reduction in the hnRNP Q level leads to slow proliferation and less susceptibility to 5-FU. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. B.C.Yoo; S-H.Hong; These two authors contributed equally to this work. Received 10 September 2008; received after revision 19 October 2008; accepted 07 November 2008     

PPAR γ partial agonist, KR-62776, inhibits adipocyte differentiation via activation of ERK

Indenone KR-62776 acts as an agonist of PPARγ without inducing obesity in animal models and cells. X-ray crystallography reveals that the indenone occupies the binding pocket in a different manner than rosiglitazone. 2-Dimensional gel-electrophoresis showed that the expression of 42 proteins was altered more than 2.0-fold between KR-62776- or rosiglitazone-treated adipocyte cells and control cells. Rosiglitazone down-regulated the expression of ERK1/2 and suppressed the phosphorylation of ERK1/2 in these cells. However, the expression of ERK1/2 was up-regulated in KR-62776-treated cells. Phosphorylated ERK1/2, activated by indenone, affects the localization of PPARγ, suggesting a mechanism for indenone-inhibition of adipogenesis in 3T3-L1 preadipocyte cells. The preadipocyte cells are treated with ERK1/2 inhibitor PD98059, a large amount of the cells are converted to adipocyte cells. These results support the conclusion that the localization of PPARγ is one of the key factors explaining the biological responses of the ligands. Received 04 March 2009; received after revision 13 March 2009; accepted 17 March 2009     

耗散介观电子谐振腔的量子kirchhoff方程与量子能谱

基于电荷离散化的事实,实现对耗散介观电子谐振腔的量子化.运用Heisenberg运动方程给出体系的量子kirchhoff方程;通过求解体系Hamilton算符的本征值方程分析和研究体系的量子能谱性质.结果表明,量子回路方程、量子能谱均与电荷的量子性质有关.     

OFDM系统中一种有效的定时和频偏估计方法

为了在没有任何导频信息的条件下,实现OFDM(orthogonal frequency division)系统中的符号定时和频偏估计算法.提出了一种重复训练后缀的OFDM信号结构,并给出了一种有效的符号定时和频偏估计方法.该方法对ZP(zero padding)-OFDM信号形式进行适当的改进,在不需要任何附加训练和导频信息的条件下,实现了符号定时和频偏的有效估计,与基于循环前缀的估计方法相比,该估计方法扩大了可估计的频率偏差范围,同时降低了运算量.     

基于信任的普适计算的动态授权模型

提出了一种联合结构的动态信任模型.在信任模型基础上,扩展了基于角色的访问控制模型.在信任模型中,将信任表示为能够体现信息空间和物理空间的特点的基本信任和动态信任的联合结构.基本信任决定对陌生实体的认证;认证完成后,根据基本信任值,用户被分配一组角色,动态信任决定角色和许可的激活,不同的动态信任值激活不同的角色和许可,因而用户的访问权限也随之变化.     

Meta-analysis of genome-wide association data and large-scale replication identifies additional susceptibility loci for type 2 diabetes

Genome-wide association (GWA) studies have identified multiple loci at which common variants modestly but reproducibly influence risk of type 2 diabetes (T2D). Established associations to common and rare variants explain only a small proportion of the heritability of T2D. As previously published analyses had limited power to identify variants with modest effects, we carried out meta-analysis of three T2D GWA scans comprising 10,128 individuals of European descent and approximately 2.2 million SNPs (directly genotyped and imputed), followed by replication testing in an independent sample with an effective sample size of up to 53,975. We detected at least six previously unknown loci with robust evidence for association, including the JAZF1 (P = 5.0 x 10(-14)), CDC123-CAMK1D (P = 1.2 x 10(-10)), TSPAN8-LGR5 (P = 1.1 x 10(-9)), THADA (P = 1.1 x 10(-9)), ADAMTS9 (P = 1.2 x 10(-8)) and NOTCH2 (P = 4.1 x 10(-8)) gene regions. Our results illustrate the value of large discovery and follow-up samples for gaining further insights into the inherited basis of T2D.     

综合环境费用的概念与计算方法研究

在分析了环境费用的概念及估价方法后 ,针对环境费用较难以估量的问题 ,提出了综合环境费用的概念 ,并在投入占用产出的框架内 ,给出了三种计算方法 .在综合环境费用的基础上 ,给出了衡量环境破坏程度的相对指标 -环境破坏度的概念 ,并对其计算方法进行了研究 .