基于混合求解算法的智能组卷研究

智能组卷是一个多约束目标的组合优化问题,针对传统算法在组卷方面存在的不足,将遗传算法和模拟退火算法相结合,提出了一种混合求解算法.新算法不仅克服了未成熟收敛,而且速度和性能都有显著提高.实验结果表明,新算法提高了组卷效率.     

人胎儿骨髓间充质干细胞的分离及生物学鉴定

通过原代细胞培养,从引产胎儿骨髓组织中分离干细胞,然后进行生物学鉴定,旨在体外建立培养胎儿骨髓干细胞的有效方法,为进一步研究干细胞奠定基础．本研究对四个月的引产胎儿骨髓组织进行原代细胞培养,采用贴壁筛选法,在含有15％胎牛血清的L-DMEM／IMDM(1:1)混和培养液中培养,7 d后细胞可长满瓶底．显微镜下观察,细胞形态均一,呈长梭形．传代后,在8代以内的细胞贴壁能力较强,生长速度较快．将其命名为BMMS-03．在第3代时,对培养的细胞进行了于细胞标志物的生物学鉴定,采用流式细胞仪对经免疫荧光染色的细胞进行检测．结果显示:97．2％的细胞呈CD105阳性反应,66．0％的细胞呈CD106阳性反应, 9．2％的细胞呈CD34阳性反应．阴性对照组阳性反应为0．5％．生物学鉴定的初步结果提示,从胎儿骨髓组织中分离培养成功的细胞为骨髓间充质干细胞,其细胞形态学特征、CD105 、CD106 和CD34-的检测结果均符合间充质干细胞的特征．本研究成功地建立了体外培养胎儿骨髓间充质干细胞的有效方法,所获得的间充质干细胞纯度较高,增殖较快,适用于干细胞生物学和组织工程学的研究．     

图算角变力矩法

力矩分配法,是工程界常用的手算结构的力学分析方法.以力矩表达结构结点角位移的量,称为角变力矩.以角变力矩为未知量,以计算图为对象利用力矩分配法概念求解超静定结构的方法,称为图算角变力矩法,即边看图边计算角变力矩的方法.图算角变力矩法,克服了力矩分配法直接待求量个数多、计算的渐近性和有截断误差的近似性等缺点,发扬了力矩分配法计算程式规范,省脑力、思路清晰等优点,成为一种直接精确的力学分析方法,为建立超静定结构普遍统一的力学分析方法奠定了基础.     

一种高效的移动IP注册协议

提出了一种安全、高效的具有匿名性的无证书公钥移动IP注册协议.新协议由于在签名过程中只使用了一个标量乘,验证过程中只使用了两个标量乘,并且没有对运算和模拟运算,因此比以前的方案效率更高.通过在新协议中重复使用初始值实现了MN与HA之间的重新同步;通过构造一个不断变化的临时账户TID来替代真实身份,真正实现了用户的匿名性;通过在通信实体的注册消息中附加随机数,抵御了重放攻击.性能分析和数值计算结果证明在提供相同级别安全性的基础上,该协议在注册时延和计算负载方面都优于现有的协议.尤其是本协议的注册延迟时间比WS Yap的减少了41.68 ms,比马华的减少了0.83 ms.     

一种频域RS-LT级联码在短波通信中的应用研究

针对短波通信中同时存在误码和丢包的特点,提出了一种频域RS码与数字喷泉码串行级联的纠错纠删编码算法,并在FPGA芯片及短波电台终端上予以实现。频域RS码作为外码,LT码作为内码,使RS码和LT码互相促进,同时实现纠删和纠错,这是单独采用两种编码方法都无法达到的。仿真表明RS-LT级联编码可提高LT码译码成功概率。同时短波信道实测结果表明,编码模块的应用可有效提高短波电台正确接收概率。     

四轮转向直接横摆力矩鲁棒集成控制仿真研究

将四轮转向与直接横摆力矩相结合,考虑模型摄动优化权函数抑制外扰,基于模型跟踪设计μ综合鲁棒集成控制器.采用J-turn操纵方式高速下验证控制器的鲁棒性,比较前轮转向直接横摆力矩,四轮转向与四轮转向直接横摆力矩三种车辆控制系统的操纵性能,仿真表明设计的四轮转向直接横摆力矩集成μ综合控制器更能有效地提高车辆闭环系统的鲁棒性,抵制外部扰动的鲁棒稳定性,且避免了H∞控制器的保守性.     

Exploration of TCM Masters Knowledge Mining

Traditional Chinese medicine （TCM） has a rich knowledge about human health and disease by its special way evolved along a very long history. As modern medicine is achieving much progress, arguments and disputes toward TCM never end. To avoid losing precious knowledge of living TCM masters, endeavors have been engaged to systematic collection of those knowledge of TCM masters, such as their growth experiences, effective practical cases toward diseases and typical therapeutic principles and methods. Knowledge mining methods have been expected to explore some useful or hidden patterns to unveil some mysteries of the TCM system. In the paper, some computerized methods are applied toward those collected materials about some living TCM masters in China mainland to show a different way of exposing essential ideas of those TCM masters by correspondence visualization which aims to help people understand TCM holistic views toward disease and body, and facilitate tacit knowledge transfer and sense-making of the essence of TCM. The work is one kind of qualitative meta-synthesis of TCM masters＇ knowledge.     

Zebrafish miR-214 modulates Hedgehog signaling to specify muscle cell fate

Numerous microRNAs (miRNAs) have been discovered in the genomes of higher eukaryotes, and functional studies indicate that they are important during development. However, little is known concerning the function of individual miRNAs. We approached this problem in zebrafish by combining identification of miRNA expression, functional analyses and experimental validation of potential targets. We show that miR-214 is expressed during early segmentation stages in somites and that varying its expression alters the expression of genes regulated by Hedgehog signaling. Inhibition of miR-214 results in a reduction or loss of slow-muscle cell types. We show that su(fu) mRNA, encoding a negative regulator of Hedgehog signaling, is targeted by miR-214. Through regulation of su(fu), miR-214 enables precise specification of muscle cell types by sharpening cellular responses to Hedgehog.     

Loss of ACTN3 gene function alters mouse muscle metabolism and shows evidence of positive selection in humans

More than a billion humans worldwide are predicted to be completely deficient in the fast skeletal muscle fiber protein alpha-actinin-3 owing to homozygosity for a premature stop codon polymorphism, R577X, in the ACTN3 gene. The R577X polymorphism is associated with elite athlete status and human muscle performance, suggesting that alpha-actinin-3 deficiency influences the function of fast muscle fibers. Here we show that loss of alpha-actinin-3 expression in a knockout mouse model results in a shift in muscle metabolism toward the more efficient aerobic pathway and an increase in intrinsic endurance performance. In addition, we demonstrate that the genomic region surrounding the 577X null allele shows low levels of genetic variation and recombination in individuals of European and East Asian descent, consistent with strong, recent positive selection. We propose that the 577X allele has been positively selected in some human populations owing to its effect on skeletal muscle metabolism.     

针对不平衡数据集的客户流失预测算法

针对客户关系管理中的客户流失预测问题进行探讨,通过对客户流失数据特点的分析,以及现有预测算法的比较,将数据挖掘方法中的随机森林算法引入客户流失预测,建立预测模型,并在实际的银行业贷款客户数据集上进行实验,得到了较好的效果.