Mechanism for the learning deficits in a mouse model of neurofibromatosis type 1.

Neurofibromatosis type I (NF1) is one of the most common single-gene disorders that causes learning deficits in humans. Mice carrying a heterozygous null mutation of the Nfl gene (Nfl(+/-) show important features of the learning deficits associated with NF1 (ref. 2). Although neurofibromin has several known properties and functions, including Ras GTPase-activating protein activity, adenylyl cyclase modulation and microtubule binding, it is unclear which of these are essential for learning in mice and humans. Here we show that the learning deficits of Nf1(+/-) mice can be rescued by genetic and pharmacological manipulations that decrease Ras function. We also show that the Nf1(+/-) mice have increased GABA (gamma-amino butyric acid)-mediated inhibition and specific deficits in long-term potentiation, both of which can be reversed by decreasing Ras function. Our results indicate that the learning deficits associated with NF1 may be caused by excessive Ras activity, which leads to impairments in long-term potentiation caused by increased GABA-mediated inhibition. Our findings have implications for the development of treatments for learning deficits associated with NF1.     

The failing heart.

Cardiomyopathies are disorders affecting heart muscle that usually result in inadequate pumping of the heart. They are the most common cause of heart failure and each year kill more than 10,000 people in the United States. In recent years, there have been breakthroughs in understanding the molecular mechanisms involved in this group of conditions, with knowledge of the genetic basis for cardiomyopathies perhaps seeing the largest advance, enabling clinicians to devise improved diagnostic strategies and preparing the stage for new therapies.     

矿石可选性预测专家系统(Ⅰ)─—系统的总体设计

建立了铅锌矿石可造性预测专家系统，运行结果良好。本篇着重论述矿石可造性领域知识的特点和知识表示方法；分析矿石可造性预测专家系统的主要特征；讨论矿石可造性预测子问题的划分、一般决策过程、推理控制策略以及系统的总体设计。     

高新技术开发区网络管理系统的设计

根据高新技术产业开发区的特点，提出了一系列有针对性的软、硬件设计。     

Function of DnaJ and DnaK as chaperones in origin-specific DNA binding by RepA

Heat-shock proteins are normal constituents of cells whose synthesis is increased on exposure to various forms of stress. They are interesting because of their ubiquity and high conservation during evolution. Two families of heat-shock proteins, hsp60s and hsp70s, have been implicated in accelerating protein folding and oligomerization and also in maintaining proteins in an unfolded state, thus facilitating membrane transport. The Escherichia coli hsp70 analogue, DnaK, and two other heat-shock proteins, DnaJ and GrpE, are required for cell viability at high temperatures and are involved in DNA replication of phage lambda and plasmids P1 and F. These three proteins are involved in replication in vitro of P1 DNA along with many host replication proteins and the P1 RepA initiator protein. RepA exists in a stable protein complex with DnaJ containing a dimer each of RepA and DnaJ. We report here that DnaK and DnaJ mediate an alteration in the P1 initiator protein, rendering it much more active for oriP1 DNA binding.     

介质腔稳频MIC射电接收的研究

本文研究的是射电望远镜中的本机振荡源、混频器和前中放三部分的混合MIC,对高Q介质稳频的反射型与反馈型两种振荡器的综合方式,理论上作了重点分析探讨,并研制成带有DRO的MIC组件。     

Abnormal pattern detected in fragile-X patients by pulsed-field gel electrophoresis.

The fragile-X syndrome is the most frequent inherited form of mental retardation, with an incidence of 1 in 1,500 males. It is characterized by the presence of a fragile site at Xq27.3 induced in vitro by folate deprivation or by inhibitors of deoxynucleotide synthesis. Its mode of inheritance is unusual for an X-linked trait, with incomplete penetrance in both males and females. Some phenotypically normal males transmit the mutation to all their daughters who rarely express any symptoms, but penetrance is high in sons and daughters of these carrier women. Genetic and physical mapping of the Xq27-q28 region has confirmed that the disease locus is located at or very near the fragile site. Hypotheses proposed to account for the abnormalities in the inheritance of the disease include sequence rearrangements by meiotic recombination or a mutation that affects reactivation of an inactive X chromosome during differentiation of female germ cells. To detect such rearrangements, or methylation changes that may reflect a locally inactive X chromosome, we used pulsed-field gel analysis of DNA from fragile-X patients with probes close to the fragile-X locus. The probe Do33 (DXS465) detected abnormal patterns in fragile-X patients, but not in normal controls or in non-expressing male transmitters.     

层流液柱吸收法测定气体在液相中的扩散系数

采用射流吸收的简捷方法，通过测定由Ｓｈｏｕｔｈｗｅｌｌ型孔板所产生的截面速度均匀的层流喷射液柱所吸收的气体量，由渗透理论所导出的模型，可计算出一定温度、压力下的微溶气体在液相中的分子扩散系数Ｄ。在温度为３０～４５℃的范围，测试结果与文献值吻合良好．     

汕头市API指数年变化规律及控制对策

对汕头市全年空气主要污染物指数年变化规律进行统计、分析 ,结果显示 :其年变化规律受季节及气象要素影响 ,可吸入颗粒物浓度偏高的主要原因与人群活动有较密切关系 .并提出相应的控制对策 :加强对机动车尾气的控制、工业污染源管理和做好垃圾有效处理、主干道定期洒水降尘及绿化固土等工作     

A New Local Search Algorithm for the Job Shop Scheduling Problem

0　IntroductionThejobshopschedulingproblemwithwhichwearecon cernedconsistsinschedulingasetofjobsonasetofma chinesfortheobjectiveofminimizingthemake span ,i.e .themaximumoftimeneededforfinishingalljobs,whichissubjecttotheconstrainsthateachjobhasafixedprocessingorderthroughthemachinesandeachmachinecanprocessatmostonejobatatime .ThisproblemisNP hardandevenisoneofthehardestcom binationaloptimizationproblems.Itiswellknownthatonlysmallsizeprobleminstancescanbesolvedwithinareasonablecomputa tionalti…