The failing heart.

Cardiomyopathies are disorders affecting heart muscle that usually result in inadequate pumping of the heart. They are the most common cause of heart failure and each year kill more than 10,000 people in the United States. In recent years, there have been breakthroughs in understanding the molecular mechanisms involved in this group of conditions, with knowledge of the genetic basis for cardiomyopathies perhaps seeing the largest advance, enabling clinicians to devise improved diagnostic strategies and preparing the stage for new therapies.     

酶促反应过程中Brigg─Haldane稳态假说的适用性

本文利用非平衡态动力学方法探讨了Ｂｒｉｇｇ—Ｈａｌｄａｎｅ稳态假说对酶促反应的适用性．结果表明，在反应过程中间并不存在着一个时间区域使稳态条件成立，并采用分支理论得到了反应运力学方程的近似解。     

Function of DnaJ and DnaK as chaperones in origin-specific DNA binding by RepA

Heat-shock proteins are normal constituents of cells whose synthesis is increased on exposure to various forms of stress. They are interesting because of their ubiquity and high conservation during evolution. Two families of heat-shock proteins, hsp60s and hsp70s, have been implicated in accelerating protein folding and oligomerization and also in maintaining proteins in an unfolded state, thus facilitating membrane transport. The Escherichia coli hsp70 analogue, DnaK, and two other heat-shock proteins, DnaJ and GrpE, are required for cell viability at high temperatures and are involved in DNA replication of phage lambda and plasmids P1 and F. These three proteins are involved in replication in vitro of P1 DNA along with many host replication proteins and the P1 RepA initiator protein. RepA exists in a stable protein complex with DnaJ containing a dimer each of RepA and DnaJ. We report here that DnaK and DnaJ mediate an alteration in the P1 initiator protein, rendering it much more active for oriP1 DNA binding.     

运输需求的特性及其分析特点

研究了运输需求的概念和特性，粗略地总结了运输需求的广泛性、多样性、派生性、空间特定性、时间特定性和部分可替代性６个主要特征，说明运输需求包括流量、流向、流程、流时和流速５个要素。并分析了运输需求的产生原因，讨论了运输需求分析的特点，论述了微观经济学需求理论用于运输需求分析的局限性。     

Abnormal pattern detected in fragile-X patients by pulsed-field gel electrophoresis.

The fragile-X syndrome is the most frequent inherited form of mental retardation, with an incidence of 1 in 1,500 males. It is characterized by the presence of a fragile site at Xq27.3 induced in vitro by folate deprivation or by inhibitors of deoxynucleotide synthesis. Its mode of inheritance is unusual for an X-linked trait, with incomplete penetrance in both males and females. Some phenotypically normal males transmit the mutation to all their daughters who rarely express any symptoms, but penetrance is high in sons and daughters of these carrier women. Genetic and physical mapping of the Xq27-q28 region has confirmed that the disease locus is located at or very near the fragile site. Hypotheses proposed to account for the abnormalities in the inheritance of the disease include sequence rearrangements by meiotic recombination or a mutation that affects reactivation of an inactive X chromosome during differentiation of female germ cells. To detect such rearrangements, or methylation changes that may reflect a locally inactive X chromosome, we used pulsed-field gel analysis of DNA from fragile-X patients with probes close to the fragile-X locus. The probe Do33 (DXS465) detected abnormal patterns in fragile-X patients, but not in normal controls or in non-expressing male transmitters.     

The detection of structural deformation errors in attitude determination

In the determination of the attitude parameters from a multi-antenna GPS array, one of the major assumptions is that the body frame is rigid at all times. If this assumption is not true then the derived attitude parameters will be in error. It is well known that in airborne platforms the wings often experience some displacement during flight, especially during periods of initializing maneouvres, such as taking off, landing,and banking. Often it is at these points in time that it is most critical to have the most precise attitude parameters.There are a number of techniques available for the detection of modeling errors.The CUSUM algorithm has successfully been implemented in the past to detect small persistent changes. In this paper the authors investigate different methods of generating the residuals, to be tested by the CUSUM algorithm, in an effort to determine which technique is best suited for the detection of structural deformation of an airborne platform. The methods investigated include monitoring the mean of the residuals generated from the difference between the known body frame coordinates, and those calculated from the derived attitude parameters. The generated residuals are then passed to a CUSUM algorithm to detect any small persistent changes. An alternative method involves transforming the generated residuals into the frequency domain through the use of the Fast Fourier Transform. The CUSUM algorithm is then used to detect any frequency changes. The final technique investigated involves transforming the generated residuals using the Haar wavelet. The wavelet coefficients are then monitored by the CUSUM algorithm in order to detect any significant change to the rigidity of the body frame.Detecting structural deformation, and quantifying the degree of deformation, during flight will ensure that these effects can be removed from the system, thus ensuring the most precise and reliable attitude parameter solutions. This paper, through a series ofsimulations, will assess the effectiveness of the above mentioned techniques for detecting structural deformation effects on a GPS multi-antenna array. These principles are then tested with experimental data.     

Lactate dehydrogenase specificity and subunit assembly in neural tissues of the teleostPhallichthys amates

Summary The tissue specificity of lactate dehydrogenase (EC 1.1.1.27) in brain and eye of the teleostPhallichthys amates was examined by acrylamide gel electrophoresis. It is suggested that subunit association is a function of gene product accessibility superimposed upon genetic restriction of assembly.     

职校学习后进生的形成原因与教育对策

在职业学校里客观上存在着极少数学习困难、成绩落后的学生，这些学生是教师日常教育教学的重点和难点。本文对学习后进生的形成原因进行比较全面的调查研究，在此基础上采取相应有效的教育对策是成功转化学习后进生的关键。     

HEp-2细胞MPM-2磷蛋白的蛋白质组学分析

MPM-2磷蛋白家族成员在有丝分裂进程中起着重要作用．为了从MPM-2磷蛋白家族层面入手研究细胞周期调控中磷蛋白的重要作用，以人上皮样喉癌细胞株HEp-2为细胞模型，运用双向电泳、免疫印迹以及质谱技术等蛋白质组学的方法对其细胞抽提物中的部分MPM-2磷蛋白进行了分析鉴定，初步确定了乙二醛酶(GlyoxalaseⅠ)、真核翻译起始因子(eIF4B)以及核质蛋白NPM等MPM-2特异识别的细胞磷蛋白，这将有助于进一步研究MPM2磷蛋白家族成员在细胞周期调控中的重要作用．     

试谈动态思维训练

在物理量相互制约的物理习题中,有一类A量取得特殊值,B量取极值问题。可称“条件极值题”。本文探讨了用《高中物理客观性命题研究》中习题训练动态思维。