全文获取类型
收费全文 | 13351篇 |
免费 | 109篇 |
国内免费 | 165篇 |
专业分类
系统科学 | 274篇 |
丛书文集 | 896篇 |
教育与普及 | 668篇 |
理论与方法论 | 42篇 |
现状及发展 | 1141篇 |
研究方法 | 1490篇 |
综合类 | 9105篇 |
自然研究 | 9篇 |
出版年
2018年 | 14篇 |
2017年 | 29篇 |
2016年 | 27篇 |
2015年 | 28篇 |
2014年 | 47篇 |
2013年 | 50篇 |
2012年 | 785篇 |
2011年 | 966篇 |
2010年 | 267篇 |
2009年 | 118篇 |
2008年 | 930篇 |
2007年 | 930篇 |
2006年 | 1017篇 |
2005年 | 1041篇 |
2004年 | 918篇 |
2003年 | 848篇 |
2002年 | 687篇 |
2001年 | 562篇 |
2000年 | 816篇 |
1999年 | 274篇 |
1998年 | 64篇 |
1997年 | 39篇 |
1996年 | 43篇 |
1995年 | 26篇 |
1994年 | 53篇 |
1993年 | 57篇 |
1992年 | 47篇 |
1991年 | 68篇 |
1990年 | 57篇 |
1989年 | 77篇 |
1988年 | 59篇 |
1987年 | 54篇 |
1986年 | 75篇 |
1985年 | 77篇 |
1984年 | 75篇 |
1983年 | 59篇 |
1982年 | 80篇 |
1981年 | 64篇 |
1980年 | 39篇 |
1979年 | 50篇 |
1971年 | 38篇 |
1970年 | 73篇 |
1966年 | 17篇 |
1959年 | 236篇 |
1958年 | 414篇 |
1957年 | 302篇 |
1956年 | 259篇 |
1955年 | 252篇 |
1954年 | 299篇 |
1948年 | 70篇 |
排序方式: 共有10000条查询结果,搜索用时 953 毫秒
941.
Quantifying nitrogen-fixation in feather moss carpets of boreal forests 总被引:21,自引:0,他引:21
Biological nitrogen (N) fixation is the primary source of N within natural ecosystems, yet the origin of boreal forest N has remained elusive. The boreal forests of Eurasia and North America lack any significant, widespread symbiotic N-fixing plants. With the exception of scattered stands of alder in early primary successional forests, N-fixation in boreal forests is considered to be extremely limited. Nitrogen-fixation in northern European boreal forests has been estimated at only 0.5 kg N ha(-1) yr(-1); however, organic N is accumulated in these ecosystems at a rate of 3 kg N ha(-1) yr(-1) (ref. 8). Our limited understanding of the origin of boreal N is unacceptable given the extent of the boreal forest region, but predictable given our imperfect knowledge of N-fixation. Herein we report on a N-fixing symbiosis between a cyanobacterium (Nostoc sp.) and the ubiquitous feather moss, Pleurozium schreberi (Bird) Mitt. that alone fixes between 1.5 and 2.0 kg N ha(-1) yr(-1) in mid- to late-successional forests of northern Scandinavia and Finland. Previous efforts have probably underestimated N-fixation potential in boreal forests. 相似文献
942.
Shoot control of root development and nodulation is mediated by a receptor-like kinase 总被引:35,自引:0,他引:35
Krusell L Madsen LH Sato S Aubert G Genua A Szczyglowski K Duc G Kaneko T Tabata S de Bruijn F Pajuelo E Sandal N Stougaard J 《Nature》2002,420(6914):422-426
In legumes, root nodule organogenesis is activated in response to morphogenic lipochitin oligosaccharides that are synthesized by bacteria, commonly known as rhizobia. Successful symbiotic interaction results in the formation of highly specialized organs called root nodules, which provide a unique environment for symbiotic nitrogen fixation. In wild-type plants the number of nodules is regulated by a signalling mechanism integrating environmental and developmental cues to arrest most rhizobial infections within the susceptible zone of the root. Furthermore, a feedback mechanism controls the temporal and spatial susceptibility to infection of the root system. This mechanism is referred to as autoregulation of nodulation, as earlier nodulation events inhibit nodulation of younger root tissues. Lotus japonicus plants homozygous for a mutation in the hypernodulation aberrant root (har1) locus escape this regulation and form an excessive number of nodules. Here we report the molecular cloning and expression analysis of the HAR1 gene and the pea orthologue, Pisum sativum, SYM29. HAR1 encodes a putative serine/threonine receptor kinase, which is required for shoot-controlled regulation of root growth, nodule number, and for nitrate sensitivity of symbiotic development. 相似文献
943.
Thiamine derivatives bind messenger RNAs directly to regulate bacterial gene expression 总被引:51,自引:0,他引:51
Although proteins fulfil most of the requirements that biology has for structural and functional components such as enzymes and receptors, RNA can also serve in these capacities. For example, RNA has sufficient structural plasticity to form ribozyme and receptor elements that exhibit considerable enzymatic power and binding specificity. Moreover, these activities can be combined to create allosteric ribozymes that are modulated by effector molecules. It has also been proposed that certain messenger RNAs might use allosteric mechanisms to mediate regulatory responses depending on specific metabolites. We report here that mRNAs encoding enzymes involved in thiamine (vitamin B(1)) biosynthesis in Escherichia coli can bind thiamine or its pyrophosphate derivative without the need for protein cofactors. The mRNA-effector complex adopts a distinct structure that sequesters the ribosome-binding site and leads to a reduction in gene expression. This metabolite-sensing regulatory system provides an example of a 'riboswitch' whose evolutionary origin might pre-date the emergence of proteins. 相似文献
944.
Tumour-derived soluble MIC ligands impair expression of NKG2D and T-cell activation 总被引:64,自引:0,他引:64
Engagement of the NKG2D receptor by tumour-associated ligands may promote tumour rejection by stimulating innate and adaptive lymphocyte responses. In humans, NKG2D is expressed on most natural killer cells, gammadelta T cells and CD8alphabeta T cells. Ligands of NKG2D include the major histocompatibility complex class I homologues MICA and MICB, which function as signals of cellular stress. These molecules are absent from most cells and tissues but can be induced by viral and bacterial infections and are frequently expressed in epithelial tumours. MIC engagement of NKG2D triggers natural killer cells and costimulates antigen-specific effector T cells. Here we show that binding of MIC induces endocytosis and degradation of NKG2D. Expression of NKG2D is reduced markedly on large numbers of tumour-infiltrating and matched peripheral blood T cells from individuals with cancer. This systemic deficiency is associated with circulating tumour-derived soluble MICA, causing the downregulation of NKG2D and in turn severe impairment of the responsiveness of tumour-antigen-specific effector T cells. This mode of T-cell silencing may promote tumour immune evasion and, by inference, compromise host resistance to infections. 相似文献
945.
946.
Acrylamide from Maillard reaction products 总被引:75,自引:0,他引:75
Stadler RH Blank I Varga N Robert F Hau J Guy PA Robert MC Riediker S 《Nature》2002,419(6906):449-450
The discovery of the adventitious formation of the potential cancer-causing agent acrylamide in a variety of foods during cooking has raised much concern, but the chemical mechanism(s) governing its production are unclear. Here we show that acrylamide can be released by the thermal treatment of certain amino acids (asparagine, for example), particularly in combination with reducing sugars, and of early Maillard reaction products (N-glycosides). Our findings indicate that the Maillard-driven generation of flavour and colour in thermally processed foods can -- under particular conditions -- be linked to the formation of acrylamide. 相似文献
947.
948.
949.
950.
Crystal structure of bacterial multidrug efflux transporter AcrB 总被引:59,自引:0,他引:59
AcrB is a major multidrug exporter in Escherichia coli. It cooperates with a membrane fusion protein, AcrA, and an outer membrane channel, TolC. We have determined the crystal structure of AcrB at 3.5 A resolution. Three AcrB protomers are organized as a homotrimer in the shape of a jellyfish. Each protomer is composed of a transmembrane region 50 A thick and a 70 A protruding headpiece. The top of the headpiece opens like a funnel, where TolC might directly dock into AcrB. A pore formed by three alpha-helices connects the funnel with a central cavity located at the bottom of the headpiece. The cavity has three vestibules at the side of the headpiece which lead into the periplasm. In the transmembrane region, each protomer has twelve transmembrane alpha-helices. The structure implies that substrates translocated from the cell interior through the transmembrane region and from the periplasm through the vestibules are collected in the central cavity and then actively transported through the pore into the TolC tunnel. 相似文献