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排序方式: 共有60条查询结果,搜索用时 15 毫秒
31.
32.
Identification of Stk6/STK15 as a candidate low-penetrance tumor-susceptibility gene in mouse and human 总被引:20,自引:0,他引:20
Ewart-Toland A Briassouli P de Koning JP Mao JH Yuan J Chan F MacCarthy-Morrogh L Ponder BA Nagase H Burn J Ball S Almeida M Linardopoulos S Balmain A 《Nature genetics》2003,34(4):403-412
Linkage analysis and haplotype mapping in interspecific mouse crosses (Mus musculus x Mus spretus) identified the gene encoding Aurora2 (Stk6 in mouse and STK15 in human) as a candidate skin tumor susceptibility gene. The Stk6 allele inherited from the susceptible M. musculus parent was overexpressed in normal cells and preferentially amplified in tumor cells from F(1) hybrid mice. We identified a common genetic variant in STK15 (resulting in the amino acid substitution F31I) that is preferentially amplified and associated with the degree of aneuploidy in human colon tumors. The Ile31 variant transforms rat1 cells more potently than the more common Phe31 variant. The E2 ubiquitin-conjugating enzyme UBE2N was a preferential binding partner of the 'weak' STK15 Phe31 variant form in yeast two-hybrid screens and in human cells. This interaction results in colocalization of UBE2N with STK15 at the centrosomes during mitosis. These results are consistent with an important role for the Ile31 variant of STK15 in human cancer susceptibility. 相似文献
33.
A transcription factor response element for gene expression during circadian night 总被引:26,自引:0,他引:26
Ueda HR Chen W Adachi A Wakamatsu H Hayashi S Takasugi T Nagano M Nakahama K Suzuki Y Sugano S Iino M Shigeyoshi Y Hashimoto S 《Nature》2002,418(6897):534-539
34.
探讨加用气流阻力负荷(FRL) 状态下流速容量(FV) 曲线检查对初检OSA 患者的意义对象为24 名健康成年男性及21 例男性阻塞性睡眠呼吸暂停(OSA) 患者经整夜多导睡眠仪检查诊断或排除OSA模拟实验结果发现只有同时加上可陷闭管及FRL 时,才出现显著吸气气流受限无FRL时,FV 曲线( 以FEF50/FIF50 比值大于1 为指标) 检查发现OSA的敏感性和特异性分别为33 .3 % 和91 .6 % ,阳性预测率为63 .6 % 加用FRL 时敏感性为76 .2 % ,特异性为75 % ,阳性预测率为72 .2 % 吸、呼气相峰值流速比值及中期峰值流速比值与OSA 严重呈显著相关联合应用加FRL及常规FL曲线检查可能对OSA 患者初筛有较高价值 相似文献
35.
Feedback repression is required for mammalian circadian clock function 总被引:13,自引:0,他引:13
Sato TK Yamada RG Ukai H Baggs JE Miraglia LJ Kobayashi TJ Welsh DK Kay SA Ueda HR Hogenesch JB 《Nature genetics》2006,38(3):312-319
36.
Nakamura T Liu YJ Nakashima H Umehara H Inoue K Matoba S Tachibana M Ogura A Shinkai Y Nakano T 《Nature》2012,486(7403):415-419
The modification of DNA by 5-methylcytosine (5mC) has essential roles in cell differentiation and development through epigenetic gene regulation. 5mC can be converted to another modified base, 5-hydroxymethylcytosine (5hmC), by the tet methylcytosine dioxygenase (Tet) family of enzymes. Notably, the balance between 5hmC and 5mC in the genome is linked with cell-differentiation processes such as pluripotency and lineage commitment. We have previously reported that the maternal factor PGC7 (also known as Dppa3, Stella) is required for the maintenance of DNA methylation in early embryogenesis, and protects 5mC from conversion to 5hmC in the maternal genome. Here we show that PGC7 protects 5mC from Tet3-mediated conversion to 5hmC by binding to maternal chromatin containing dimethylated histone H3 lysine 9 (H3K9me2) in mice. In addition, imprinted loci that are marked with H3K9me2 in mature sperm are protected by PGC7 binding in early embryogenesis. This type of regulatory mechanism could be involved in DNA modifications in somatic cells as well as in early embryos. 相似文献
37.
Miyamichi K Amat F Moussavi F Wang C Wickersham I Wall NR Taniguchi H Tasic B Huang ZJ He Z Callaway EM Horowitz MA Luo L 《Nature》2011,472(7342):191-196
In the mouse, each class of olfactory receptor neurons expressing a given odorant receptor has convergent axonal projections to two specific glomeruli in the olfactory bulb, thereby creating an odour map. However, it is unclear how this map is represented in the olfactory cortex. Here we combine rabies-virus-dependent retrograde mono-trans-synaptic labelling with genetics to control the location, number and type of 'starter' cortical neurons, from which we trace their presynaptic neurons. We find that individual cortical neurons receive input from multiple mitral cells representing broadly distributed glomeruli. Different cortical areas represent the olfactory bulb input differently. For example, the cortical amygdala preferentially receives dorsal olfactory bulb input, whereas the piriform cortex samples the whole olfactory bulb without obvious bias. These differences probably reflect different functions of these cortical areas in mediating innate odour preference or associative memory. The trans-synaptic labelling method described here should be widely applicable to mapping connections throughout the mouse nervous system. 相似文献
38.
39.
阻塞性睡眠呼吸暂停患者的上气道狭窄与头姿变化 总被引:7,自引:2,他引:5
探讨阻塞性睡眠呼吸暂停(Obstructivesleepapnea,OSA)患者上气道狭窄的程度、头姿改变情况,以及这些形态学指标与病理生理学参数之间的关系。研究对象为86例男性OSA患者及37名健康男性。经整夜多导睡眠仪检查诊断或排除OSA;采用数字化技术摄取受试者头颅侧位片,并用NIHImage软件测定上气道口径、面积及头姿指标共15项。OSA患者各水平上气道前后径均显著小于对照组;经年龄及身高体重指数校正后,上述结果不变。重症OSA患者呈显著头颅前倾。头姿变化指标与呼吸暂停指数及多个水平的气道前后径呈显著相关。亚洲人种OSA患者存在明显及广泛的上气道狭窄;随OSA程度加重及上气道阻塞程度加重,患者可能出现代偿性头姿改变 相似文献
40.
Summary Ovarian extracts prepared from immature mice injected with human chorionic gonadotropin (hCG) and pregnant mare serum gonadotropin (PMSG) were assayed for angiogenic activity. The assay method consisted of implanting a film coated with ovarian extracts to the lateral wall of the m.rectus abdominus of a mouse for 20 days and examining the site for vascularization. The higher angiogenic activity obtained with PMSG-treated extract may be related to its follicle stimulating activity.Acknowledgment. Part of this work was supported by a grant from The Ministry of Education, Japan (No. 576165). 相似文献