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81.
82.
论文主要研究磁场和Dzyaloshinskii-Moriya(DM)相互作用对以三比特海森堡XXZ自旋链为量子信道进行隐形传态的影响。通过平均保真度的解析表达式分析了不同参数对量子隐形传态的影响,我们发现各向同性参数和各向异性参数对隐形传态起积极的作用,但是磁场和DM相互作用参数却表现出消极的作用。此外,这个模型中只有FM中的平均保真度可以超过经典最大值。 相似文献
83.
Induction of somatic hypermutation in immunoglobulin genes is dependent on DNA polymerase iota 总被引:12,自引:0,他引:12
Somatic hypermutation of immunoglobulin genes is a unique, targeted, adaptive process. While B cells are engaged in germinal centres in T-dependent responses, single base substitutions are introduced in the expressed Vh/Vl genes to allow the selection of mutants with a higher affinity for the immunizing antigen. Almost every possible DNA transaction has been proposed to explain this process, but each of these models includes an error-prone DNA synthesis step that introduces the mutations. The Y family of DNA polymerases--pol eta, pol iota, pol kappa and rev1--are specialized for copying DNA lesions and have high rates of error when copying a normal DNA template. By performing gene inactivation in a Burkitt's lymphoma cell line inducible for hypermutation, we show here that somatic hypermutation is dependent on DNA polymerase iota. 相似文献
84.
Oaks JL Gilbert M Virani MZ Watson RT Meteyer CU Rideout BA Shivaprasad HL Ahmed S Chaudhry MJ Arshad M Mahmood S Ali A Khan AA 《Nature》2004,427(6975):630-633
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86.
Laguette N Sobhian B Casartelli N Ringeard M Chable-Bessia C Ségéral E Yatim A Emiliani S Schwartz O Benkirane M 《Nature》2011,474(7353):654-657
The primate lentivirus auxiliary protein Vpx counteracts an unknown restriction factor that renders human dendritic and myeloid cells largely refractory to HIV-1 infection. Here we identify SAMHD1 as this restriction factor. SAMHD1 is a protein involved in Aicardi-Goutières syndrome, a genetic encephalopathy with symptoms mimicking congenital viral infection, that has been proposed to act as a negative regulator of the interferon response. We show that Vpx induces proteasomal degradation of SAMHD1. Silencing of SAMHD1 in non-permissive cell lines alleviates HIV-1 restriction and is associated with a significant accumulation of viral DNA in infected cells. Concurrently, overexpression of SAMHD1 in sensitive cells inhibits HIV-1 infection. The putative phosphohydrolase activity of SAMHD1 is probably required for HIV-1 restriction. Vpx-mediated relief of restriction is abolished in SAMHD1-negative cells. Finally, silencing of SAMHD1 markedly increases the susceptibility of monocytic-derived dendritic cells to infection. Our results demonstrate that SAMHD1 is an antiretroviral protein expressed in cells of the myeloid lineage that inhibits an early step of the viral life cycle. 相似文献
87.
Smeulders MJ Barends TR Pol A Scherer A Zandvoort MH Udvarhelyi A Khadem AF Menzel A Hermans J Shoeman RL Wessels HJ van den Heuvel LP Russ L Schlichting I Jetten MS Op den Camp HJ 《Nature》2011,478(7369):412-416
Extremophilic organisms require specialized enzymes for their exotic metabolisms. Acid-loving thermophilic Archaea that live in the mudpots of volcanic solfataras obtain their energy from reduced sulphur compounds such as hydrogen sulphide (H(2)S) and carbon disulphide (CS(2)). The oxidation of these compounds into sulphuric acid creates the extremely acidic environment that characterizes solfataras. The hyperthermophilic Acidianus strain A1-3, which was isolated from the fumarolic, ancient sauna building at the Solfatara volcano (Naples, Italy), was shown to rapidly convert CS(2) into H(2)S and carbon dioxide (CO(2)), but nothing has been known about the modes of action and the evolution of the enzyme(s) involved. Here we describe the structure, the proposed mechanism and evolution of a CS(2) hydrolase from Acidianus A1-3. The enzyme monomer displays a typical β-carbonic anhydrase fold and active site, yet CO(2) is not one of its substrates. Owing to large carboxy- and amino-terminal arms, an unusual hexadecameric catenane oligomer has evolved. This structure results in the blocking of the entrance to the active site that is found in canonical β-carbonic anhydrases and the formation of a single 15-?-long, highly hydrophobic tunnel that functions as a specificity filter. The tunnel determines the enzyme's substrate specificity for CS(2), which is hydrophobic. The transposon sequences that surround the gene encoding this CS(2) hydrolase point to horizontal gene transfer as a mechanism for its acquisition during evolution. Our results show how the ancient β-carbonic anhydrase, which is central to global carbon metabolism, was transformed by divergent evolution into a crucial enzyme in CS(2) metabolism. 相似文献
88.
AHMAD Salman 《科学通报(英文版)》2011,56(25):2674-2678
Sloshing is a kind of fluid motion inside partially filled containers. In spacecraft and other partially filled moving containers, sloshing plays an important role. The contact line between the fluid and solid boundary affects the fluid movement and sloshing during motion. A physical model for steady fluid flow with a partial slip boundary is presented and equations for this model are derived for cylindrical (tube-shaped) tanks. This gives a nonlinear system of differential equations that is solved numerically by using a Successive Over-Relaxation (SOR) technique and graphical results are shown. Variations in steady fluid flow are observed with changes in the slip length and some useful results are derived. The effects on the microscopic radius of the fluid layer in a capillary tube are also shown through graphical results. 相似文献
89.
Kanasaki K Palmsten K Sugimoto H Ahmad S Hamano Y Xie L Parry S Augustin HG Gattone VH Folkman J Strauss JF Kalluri R 《Nature》2008,453(7198):1117-1121
Despite intense investigation, mechanisms that facilitate the emergence of the pre-eclampsia phenotype in women are still unknown. Placental hypoxia, hypertension, proteinuria and oedema are the principal clinical features of this disease. It is speculated that hypoxia-driven disruption of the angiogenic balance involving vascular endothelial growth factor (VEGF)/placenta-derived growth factor (PLGF) and soluble Fms-like tyrosine kinase-1 (sFLT-1, the soluble form of VEGF receptor 1) might contribute to some of the maternal symptoms of pre-eclampsia. However, pre-eclampsia does not develop in all women with high sFLT-1 or low PLGF levels, and it also occurs in some women with low sFLT-1 and high PLGF levels. Moreover, recent experiments strongly suggest that several soluble factors affecting the vasculature are probably elevated because of placental hypoxia in the pre-eclamptic women, indicating that upstream molecular defect(s) may contribute to pre-eclampsia. Here we show that pregnant mice deficient in catechol-O-methyltransferase (COMT) show a pre-eclampsia-like phenotype resulting from an absence of 2-methoxyoestradiol (2-ME), a natural metabolite of oestradiol that is elevated during the third trimester of normal human pregnancy. 2-ME ameliorates all pre-eclampsia-like features without toxicity in the Comt(-/-) pregnant mice and suppresses placental hypoxia, hypoxia-inducible factor-1alpha expression and sFLT-1 elevation. The levels of COMT and 2-ME are significantly lower in women with severe pre-eclampsia. Our studies identify a genetic mouse model for pre-eclampsia and suggest that 2-ME may have utility as a plasma and urine diagnostic marker for this disease, and may also serve as a therapeutic supplement to prevent or treat this disorder. 相似文献