Cellular responses to mild heat stress

Since its discovery in 1962 by Ritossa, the heat shock response has been extensively studied by a number of investigators to understand the molecular mechanism underlying the cellular response to heat stress. The most well characterized heat shock response is induction of the heat shock proteins that function as molecular chaperones and exert cell cycle regulatory and anti-apoptotic activities. While most investigators have focused their studies on the toxic effects of heat stress in organisms such as severe heat stress-induced cell cycle arrest and apoptosis, the cellular response to fever-ranged mild heat stress has been rather underestimated. However, the cellular response to mild heat stress is likely to be more important in a physiological sense than that to severe heat stress because the body temperature of homeothermic animals increases by only 1–2°C during febrile diseases. Here we provide information that mild heat stress does have some beneficial role in organisms via positively regulating cell proliferation and differentiation, and immune response in mammalian cells.Received 14 May 2004; received after revision 2 August 2004; accepted 16 August 2004     

Laser-induced excitation of fluorescein isothiocyanate in the immunofluorescence

Zusammenfassung Methode für die Anregung des FITC in der Immunofluoreszenz mit Argon-Laser-Licht, die eine sehr starke Fluoreszenz ermöglicht. Diese Methode wird in dem FTA-ABS-Test für Syphilis benutzt.     

Regulation of the retinal interphotoreceptor matrix Na by the retinal pigment epithelium during the light response

We examined the rabbit retinal pigment epithelium (RPE) for Na transport properties which would allow it to buffer undesirable changes in Na concentrations in the interphotoreceptor matrix (IPM) during light and dark cycles. The RPE is selectivity permeable to sodium. Open and short circuit transport studies with RPE indicate a circulating (choroid to retina and back) Na current which does not compromise the electrical integrity of the blood brain barrier but together with the Na permeselectivity is of sufficient magnitude to buffer both upwards and downwards movements of IPM [Na] during light or dark responses.     

Immunohistochemical demonstration of glucagon in an A2-cell carcinoma

Zusammenfassung Glucagon ist durch Immunohisto-fluoreszenz in einem A₂-Zell-Carcinom der menschlichen Bauchspeicheldrüse und in Metastasen in der Leber entdeckt worden.

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Acknowledgments. Grant support from the Swedish Medical Research Council No. B73-04X-1007-08 and B73-12X-102-09B, Albert Påhlsson's Foundation, Riksföreningen mot Cancer, «Fonden för Stor-Köpenhamn, Grönland och Färöarna», and «Landsföreningen för kräftans bekämpelse».     

Epicatechin can cause the seedling growth inhibitor,nagilactone E,to induce growth stimulation

Summary A possible new role for the flavonoid (–)-epicatechin (II) is described. It has no growth effects on its own, but when it is added to lettuce and rice seeds together with the known seedling growth inhibitor nagilactone E (I), the growth inhibitor activity ofI can cease and growth stimulation can be observed.     

Neurogenic responses of resistance and capacitance vessels

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Calcium alginate: A new approach in the artificial culturing of insects,applied toSpodoptera littoralis (Boisduval)

Résumé La réaction entre l'alginate de soude et les ions de calcium a été utilisée dans la préparation à froid d'un gel nutritif servant à l'élevage artificiel deSpodoptera littoralis (Boisduval).