Overexpression of SUMO perturbs the growth and development of <Emphasis Type="Italic">Caenorhabditis elegans</Emphasis>

Small ubiquitin-related modifiers (SUMOs) are important regulator proteins. Caenorhabditis elegans contains a single SUMO ortholog, SMO-1, necessary for the reproduction of C. elegans. In this study, we constructed transgenic C. elegans strains expressing human SUMO-1 under the control of pan-neuronal (aex-3) or pan-muscular (myo-4) promoter and SUMO-2 under the control of myo-4 promoter. Interestingly, muscular overexpression of SUMO-1 or -2 resulted in morphological changes of the posterior part of the nematode. Movement, reproduction and aging of C. elegans were perturbed by the overexpression of SUMO-1 or -2. Genome-wide expression analyses revealed that several genes encoding components of SUMOylation pathway and ubiquitin-proteasome system were upregulated in SUMO-overexpressing nematodes. Since muscular overexpression of SMO-1 also brought up reproductive and mobility perturbations, our results imply that the phenotypes were largely due to an excess of SUMO, suggesting that a tight control of SUMO levels is important for the normal development of multicellular organisms.     

Meaning of Life in Fragile Wit(h)nessing: On Experiencing Radical Uniqueness as Gift and Grace

In this comment-response Mikael Lindfelt makes some suggestions to how one could develop the argument for wit(h)nessing as experiencing meaningfulness in life as put forward by Nicole Note and Emilie Van Deale. While being positive to the main phenomenological approach, and especially the dialectical relational aspect of the phenomenological argument, Lindfelt uses Alain Badiou’s talk of Event in trying both to develop the phenomenological argument and to point out some idealistic tendencies in the line of the argument. Lindfelt suggests that the aspect of uniqueness in the relational experience of the other should be taken to more radically than suggested by Note and Van Deale. By pointing out the dialectical fragility of the Event of wit(h)nessing Lindfelt is arguing for that the concept of respect could be more utilized in arguing for the experience of meaning seen as a gift.