A revision of the Patagonian predaceous midges of the subgenus Acanthohelea of Stilobezzia excluding the S. (A.) edwardsi group (Diptera: Ceratopogonidae)

In this revision of the Patagonian predaceous midges of Stilobezzia (Acanthohelea) (Diptera: Ceratopogonidae), the seven previously known species, Stilobezzia bicinctipes Ingram and Macfie, S. hirsuta I. and M, S. ornaticrus I. and M, S. patagonica I. and M, S. rava I. and M, S. succinea I. and M. and S. varia I. and M. are redescribed, illustrated and their distributions are expanded. Diagnoses are provided for Stilobezzia curvistyla Cazorla and Spinelli, S. longisternalis Cazorla and Spinelli and S. nigerrima I. and M, and the male of S. bicinctipes and the female of S. ornaticrus are described for the first time. Seven new species are described with photographs: S. estepae, S. mapuche, S. megatheca, S. monomorphica, S. pabloi, S. spinosa and S. tridentis. The species are illustrated by photographs, and a key to males and females is provided as well as distributional maps for all species.

http://zoobank.org/urn:lsid:zoobank.org:pub:CCEB2CB7-2FB3-4ABF-BCD4-75021AD6C338     

Viperin mRNA is a novel target for the human RNase MRP/RNase P endoribonuclease

RNase MRP is a conserved endoribonuclease, in humans consisting of a 267-nucleotide RNA associated with 7–10 proteins. Mutations in its RNA component lead to several autosomal recessive skeletal dysplasias, including cartilage-hair hypoplasia (CHH). Because the known substrates of mammalian RNase MRP, pre-ribosomal RNA, and RNA involved in mitochondrial DNA replication are not likely involved in CHH, we analyzed the effects of RNase MRP (and the structurally related RNase P) depletion on mRNAs using DNA microarrays. We confirmed the upregulation of the interferon-inducible viperin mRNA by RNAi experiments and this appeared to be independent of the interferon response. We detected two cleavage sites for RNase MRP/RNase P in the coding sequence of viperin mRNA. This is the first study providing direct evidence for the cleavage of a mRNA by RNase MRP/RNase P in human cells. Implications for the involvement in the pathophysiology of CHH are discussed.     

The Systems Approach to the Pluralist Theory of International Relations: A Case Study of the European Union

This article aims at introducing concepts related to the systems approach applied to the international system. First, the authors describe the systems view considering General Systems Theory in order to conceptualize the international systems as well as the Pluralist Theory of International Relations. Based on General Systems Theory, the authors analyze the international systems from the perspective of the Pluralist Theory of International Relations, using a case study on the European Union. The pluralist image of international systems presents important similarities to Systems Theory, as it analyzes the international context, characterized by variety, complexity and dynamics and uses the concepts of object, attribute, inputs and outputs, environment, motivations and process, which enable a better understanding of the international system.

Fluctuating asymmetry in populations of the South American frog Physalaemus cuvieri (Leptodactylidae) in areas with different degrees of disturbance

Methods to measure and monitor wild populations are important conservation tools in areas affected by anthropogenic disturbances. We compared three populations of the South American frog Physalaemus cuvieri Fitzinger, 1826 (Leptodactylidae) from areas with different degrees of anthropogenic disturbance in regard to their degrees of fluctuating asymmetry (FA). We selected four morphological characters (radio-ulna, femur, tibia-fibula and digit length) and compared two populations from relatively pristine areas with one population located in a highly urbanised and disturbed area. We found a significantly higher level of FA for the digit length, p < 0.05, in the population suffering the highest level of anthropogenic disturbance in relation to the two populations located in the pristine areas. There are no significant differences in the three populations in regard to FA for the radio-ulna, femur and tibia-fibula. The absence of FA in these three measurements might indicate a negative effect of limb asymmetry on this species survivorship. Our study provides a good example of the use of FA as indicator of environmental stress. However, this result must be viewed with some caution, since we observed FA in only one morphological character.     

Cross-presentation by intercellular peptide transfer through gap junctions

Major histocompatibility complex (MHC) class I molecules present peptides that are derived from endogenous proteins. These antigens can also be transferred to professional antigen-presenting cells in a process called cross-presentation, which precedes initiation of a proper T-cell response; but exactly how they do this is unclear. We tested whether peptides can be transferred directly from the cytoplasm of one cell into the cytoplasm of its neighbour through gap junctions. Here we show that peptides with a relative molecular mass of up to approximately 1,800 diffuse intercellularly through gap junctions unless a three-dimensional structure is imposed. This intercellular peptide transfer causes cytotoxic T-cell recognition of adjacent, innocent bystander cells as well as activated monocytes. Gap-junction-mediated peptide transfer is restricted to a few coupling cells owing to the high cytosolic peptidase activity. We present a mechanism of antigen acquisition for cross-presentation that couples the antigen presentation system of two adjacent cells and is lost in most tumours: gap-junction-mediated intercellular peptide coupling for presentation by bystander MHC class I molecules and transfer to professional antigen presenting cells for cross-priming.     

Functional profiling of the Saccharomyces cerevisiae genome

Determining the effect of gene deletion is a fundamental approach to understanding gene function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype are often missed. We systematically constructed a nearly complete collection of gene-deletion mutants (96% of annotated open reading frames, or ORFs) of the yeast Saccharomyces cerevisiae. DNA sequences dubbed 'molecular bar codes' uniquely identify each strain, enabling their growth to be analysed in parallel and the fitness contribution of each gene to be quantitatively assessed by hybridization to high-density oligonucleotide arrays. We show that previously known and new genes are necessary for optimal growth under six well-studied conditions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment. Less than 7% of genes that exhibit a significant increase in messenger RNA expression are also required for optimal growth in four of the tested conditions. Our results validate the yeast gene-deletion collection as a valuable resource for functional genomics.     

Identification of hemocyanin in the cyanocytes ofCarcinus maenas

Riassunto Nel tessuto emopoietico diCarcinus maenas sono state identificate le cellule che sintetizzano l'emocianina. Queste cellule contengono materiale che al microscopio elettronico presenta un aspetto granulare con particelle di dimensioni costanti simili a quelle dell'emocianina circolante e che in molti punti sono organizzate in una struttura cristallina. L'identità di questo materiale con l'emocianina è stata dimostrata col metodo dell'immunofluorescenza.

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Thanks are due to Mr.G. Tognon for technical assistance; to Prof.P. Omodeo for advice and criticism and to Prof.V. Albergoni and Dr.P. Burighel for help and discussion.