Ultrastructural identification of the nucleolus organizer by the silver staining technic]

The nucleolus organizer regions can be selectively stained in metaphase chromosome preparations by the Goodpasture and Bloom's technique which was adapted to electron microscopy analysis of cells during interphase. Using this technique, a selective accumulation of silver grains was observed over nucleolus light areas. This selective accumulation allows the identification of the interphase fibrillar centers as the nucleolus organizer regions. Ultrastructural relationships between fibrillar centers and dense fibrillar component are discussed.     

The role of forward‐ and backward‐looking information for inflation expectations formation

Assuming that private forecasters learn inflation dynamics to form their inflation expectations and that they believe a hybrid New Keynesian Phillips curve (NKPC) to capture the true data‐generating process of inflation, we aim at establishing the role of backward‐ and forward‐looking information in the inflation expectation formation process. We find that longer term expectations are crucial in shaping shorter horizon expectations. While the influence of backward‐looking information seems to diminish over time, we do not find evidence of a structural break in the expectation formation process of professional forecasters. Our results further suggest that the weight put on longer term expectations does not solely reflect a mean‐reverting process to trend inflation. Rather, it might also capture beliefs about the central bank's long‐run inflation target and its credibility to achieve inflation stabilization.     

Iterative Reconstruction for Transmission Tomography on GPU Using Nvidia CUDA

The iterative reconstruction algorithms for X-ray CT image reconstruction suffer from their high computational cost.Recently Nvidia releases common unified device architecture(CUDA),allowing developers to access to the processing power of Nvidia graphical processing units(GPUs),in order to perform general purpose computations.The use of the GPU,as an alternative computation platform,allows decreasing processing times,for parallel algorithms.This paper aims to demonstrate the feasibility of such an implement...     

Abundance and diversity of microbial life in ocean crust

Oceanic lithosphere exposed at the sea floor undergoes seawater-rock alteration reactions involving the oxidation and hydration of glassy basalt. Basalt alteration reactions are theoretically capable of supplying sufficient energy for chemolithoautotrophic growth. Such reactions have been shown to generate microbial biomass in the laboratory, but field-based support for the existence of microbes that are supported by basalt alteration is lacking. Here, using quantitative polymerase chain reaction, in situ hybridization and microscopy, we demonstrate that prokaryotic cell abundances on seafloor-exposed basalts are 3-4 orders of magnitude greater than in overlying deep sea water. Phylogenetic analyses of basaltic lavas from the East Pacific Rise (9 degrees N) and around Hawaii reveal that the basalt-hosted biosphere harbours high bacterial community richness and that community membership is shared between these sites. We hypothesize that alteration reactions fuel chemolithoautotrophic microorganisms, which constitute a trophic base of the basalt habitat, with important implications for deep-sea carbon cycling and chemical exchange between basalt and sea water.     

Comparing partitions

The problem of comparing two different partitions of a finite set of objects reappears continually in the clustering literature. We begin by reviewing a well-known measure of partition correspondence often attributed to Rand (1971), discuss the issue of correcting this index for chance, and note that a recent normalization strategy developed by Morey and Agresti (1984) and adopted by others (e.g., Miligan and Cooper 1985) is based on an incorrect assumption. Then, the general problem of comparing partitions is approached indirectly by assessing the congruence of two proximity matrices using a simple cross-product measure. They are generated from corresponding partitions using various scoring rules. Special cases derivable include traditionally familiar statistics and/or ones tailored to weight certain object pairs differentially. Finally, we propose a measure based on the comparison of object triples having the advantage of a probabilistic interpretation in addition to being corrected for chance (i.e., assuming a constant value under a reasonable null hypothesis) and bounded between ±1.William H.E. Day was Acting Editor for the reviewing of this paper. We are grateful to him, Ove Frank, Charles Lewis, Glenn W. Milligan, Ivo Molenaar, Stanley S. Wasserman, and anonymous referees for helpful suggestions. Lynn Bilger and Tom Sharpe provided competent technical assistance. Partial support of Phipps Arabie's participation in this research was provided by NSF Grant SES 8310866 and ONR Contract N00014-83-K-0733.     

Factors influencing fish assemblages of a high-elevation desert stream system in Wyoming

Seven fish species were found in the Bitter Creek drainage of southwest Wyoming, but only speckled dace ( Rhinichthys osculus ), flannelmouth sucker ( Catostomus latipinnis ), and mountain sucker ( Catostomus platyrhynchus ) were indigenous. No relationships were found between fish standing stocks and habitat features, but species richness was related to elevation and stream width. No fish were found above an elevation of 2192 m. Only the most downstream study read had more than three species present. Two indigenous species, speckled dace and mountain sucker, and a nonnative species, fathead minnow ( Pimephales promelas ), were predominant fishes in the drainage. These three species withstand intermittent stream flows that are common in the drainage.     

Reproductive characteristics of two kokanee stocks in tributaries to Flaming Gorge Reservoir, Utah and Wyoming

The charaeteristics of kokanee ( Oncorhynchus nerka ) spawning stocks in the Green River and Sheep Creek (tributaries to Flaming Gorge Reservoir) are described as observed in fall 1985 and spring 1986; the time of spawning; length, age, and sex structure of the stocks; fecundity and egg retention; density of eggs in redds; and the timing of downstream drift of emerging fry. The time of spawning, length-frequency distributions of spawning fish, and egg density in redds differed between the two stocks, but other differences were not observed.     

Mutations in INVS encoding inversin cause nephronophthisis type 2, linking renal cystic disease to the function of primary cilia and left-right axis determination

Nephronophthisis (NPHP), an autosomal recessive cystic kidney disease, leads to chronic renal failure in children. The genes mutated in NPHP1 and NPHP4 have been identified, and a gene locus associated with infantile nephronophthisis (NPHP2) was mapped. The kidney phenotype of NPHP2 combines clinical features of NPHP and polycystic kidney disease (PKD). Here, we identify inversin (INVS) as the gene mutated in NPHP2 with and without situs inversus. We show molecular interaction of inversin with nephrocystin, the product of the gene mutated in NPHP1 and interaction of nephrocystin with beta-tubulin, a main component of primary cilia. We show that nephrocystin, inversin and beta-tubulin colocalize to primary cilia of renal tubular cells. Furthermore, we produce a PKD-like renal cystic phenotype and randomization of heart looping by knockdown of invs expression in zebrafish. The interaction and colocalization in cilia of inversin, nephrocystin and beta-tubulin connect pathogenetic aspects of NPHP to PKD, to primary cilia function and to left-right axis determination.     

Membrane biology and signal transduction

Posters

Membrane biology and signal transduction