Genome-wide association study of flowering time and grain yield traits in a worldwide collection of rice germplasm

A high-density haplotype map recently enabled a genome-wide association study (GWAS) in a population of indica subspecies of Chinese rice landraces. Here we extend this methodology to a larger and more diverse sample of 950 worldwide rice varieties, including the Oryza sativa indica and Oryza sativa japonica subspecies, to perform an additional GWAS. We identified a total of 32 new loci associated with flowering time and with ten grain-related traits, indicating that the larger sample increased the power to detect trait-associated variants using GWAS. To characterize various alleles and complex genetic variation, we developed an analytical framework for haplotype-based de novo assembly of the low-coverage sequencing data in rice. We identified candidate genes for 18 associated loci through detailed annotation. This study shows that the integrated approach of sequence-based GWAS and functional genome annotation has the potential to match complex traits to their causal polymorphisms in rice.     

A genome-wide association study in Han Chinese identifies new susceptibility loci for ankylosing spondylitis

To identify susceptibility loci for ankylosing spondylitis, we performed a two-stage genome-wide association study in Han Chinese. In the discovery stage, we analyzed 1,356,350 autosomal SNPs in 1,837 individuals with ankylosing spondylitis and 4,231 controls; in the validation stage, we analyzed 30 suggestive SNPs in an additional 2,100 affected individuals and 3,496 controls. We identified two new susceptibility loci between EDIL3 and HAPLN1 at 5q14.3 (rs4552569; P = 8.77 × 10(-10)) and within ANO6 at 12q12 (rs17095830; P = 1.63 × 10(-8)). We also confirmed previously reported associations in Europeans within the major histocompatibility complex (MHC) region (top SNP, rs13202464; P < 5 × 10(-324)) and at 2p15 (rs10865331; P = 1.98 × 10(-8)). We show that rs13202464 within the MHC region mainly represents the risk effect of HLA-B*27 variants (including HLA-B*2704, HLA-B*2705 and HLA-B*2715) in Chinese. The two newly discovered loci implicate genes related to bone formation and cartilage development, suggesting their potential involvement in the etiology of ankylosing spondylitis.     

Frequent mutations of chromatin remodeling genes in transitional cell carcinoma of the bladder

Transitional cell carcinoma (TCC) is the most common type of bladder cancer. Here we sequenced the exomes of nine individuals with TCC and screened all the somatically mutated genes in a prevalence set of 88 additional individuals with TCC with different tumor stages and grades. In our study, we discovered a variety of genes previously unknown to be mutated in TCC. Notably, we identified genetic aberrations of the chromatin remodeling genes (UTX, MLL-MLL3, CREBBP-EP300, NCOR1, ARID1A and CHD6) in 59% of our 97 subjects with TCC. Of these genes, we showed UTX to be altered substantially more frequently in tumors of low stages and grades, highlighting its potential role in the classification and diagnosis of bladder cancer. Our results provide an overview of the genetic basis of TCC and suggest that aberration of chromatin regulation might be a hallmark of bladder cancer.     

Drosophila Piwi functions in Hsp90-mediated suppression of phenotypic variation

Canalization, also known as developmental robustness, describes an organism's ability to produce the same phenotype despite genotypic variations and environmental influences. In Drosophila, Hsp90, the trithorax-group proteins and transposon silencing have been previously implicated in canalization. Despite this, the molecular mechanism underlying canalization remains elusive. Here using a Drosophila eye-outgrowth assay sensitized by the dominant Kr(irregular facets-1)(Kr(If-1)) allele, we show that the Piwi-interacting RNA (piRNA) pathway, but not the short interfering RNA or micro RNA pathway, is involved in canalization. Furthermore, we isolated a protein complex composed of Hsp90, Piwi and Hop, the Hsp70/Hsp90 organizing protein homolog, and we demonstrated the function of this complex in canalization. Our data indicate that Hsp90 and Hop regulate the piRNA pathway through Piwi to mediate canalization. Moreover, they point to epigenetic silencing of the expression of existing genetic variants and the suppression of transposon-induced new genetic variation as two major mechanisms underlying piRNA pathway-mediated canalization.     

A Hierarchical Ensemble Learning Framework for Energy-Efficient Automatic Train Driving

Railway transportation plays an important role in modern society. As China's massive railway transportation network continues to grow in total mileage and operation density, the energy consumption of trains becomes a serious concern. For any given route, the geographic characteristics are known a priori, but the parameters(e.g., loading and marshaling) of trains vary from one trip to another. An extensive analysis of the train operation data suggests that the control gear operation of trains is the most important factor that affects the energy consumption. Such an observation determines that the problem of energy-efficient train driving has to be addressed by considering both the geographic information and the trip parameters. However, the problem is difficult to solve due to its high dimension, nonlinearity, complex constraints, and time-varying characteristics. Faced with these difficulties, we propose an energy-efficient train control framework based on a hierarchical ensemble learning approach. Through hierarchical refinement, we learn prediction models of speed and gear. The learned models can be used to derive optimized driving operations under real-time requirements. This study uses random forest and bagging – REPTree as classification algorithm and regression algorithm, respectively. We conduct an extensive study on the potential of bagging, decision trees, random forest, and feature selection to design an effective hierarchical ensemble learning framework. The proposed framework was testified through simulation. The average energy consumption of the proposed method is over 7% lower than that of human drivers.     

TANGO与AUTOCAD接口研究及软件设计

本文分析了TANGO自动布线软件中的PCB文件和AUTOCAD中的DXF文件的构成及其数据格式,对这两种文件间的转换进行了深入的研究,从而找出了它们之间接口的途径,这种接口的实现成功地解决了工程上电路印制板图纸的标准化问题。最后,笔者用C语言实现了接口软件的设计。     

论10 kV环网柜的二次侧核相

为有效提高10 kV配电系统供电可靠性,确保变电所、线路部分检修或故障时减少线路停电次数、缩小停电范围,笔者结合个人多年的实践工作,针对10 kV配网线路的二次核相进行了阐述.     

A Heuristic for the Job Scheduling Problem with a Common Due Window on Parallel and Non-Identical Machines

1. INTroDUCTIONThe emergence of JIT (Just in Time) management tee~gy opens up a recency research field for the jobscheduling and production managemellt. As its farther develOPment, the earnness-tardiness job schedulingproblem becomes a hot research point over y6ars. conference [1] gives a review on job scheduling problem withearnness and tardiness penalties. Reference [2] p~s another scheduling problem for minimizing the rangeof lateness on a single machine, and reference [3] giVes a he…     

江西省白炭黑工业发展途径的探讨

概述了国内外白炭黑工业的发展现状和应用情况,论述了白炭黑的性质及制备方法.指出了我省白炭黑工业目前存在的问题,并提出了具体的建议.