A bioinformatic evaluation of potential allergenicity of 85 candidate genes in transgenic organisms

The potential of genetically modified organisms(GMOs) to increase allergenicity or predispositions to allergies has attracted much attention.An approach that will properly and holistically evaluate the allergenicity of GMOs is yet to be found.Here,85 transgenes that have been reported in both international and domestic studies during recent years are summarized case by case;49 of the transformed genes were from plant sources and 36 were from animal sources.EVALLERTM,a web server for the in silico assessment of potential protein allergenicity,was used to evaluate the potential of the transgenic proteins as allergens.The biomedical journals listed in Highwire(http://highwire.stanford.edu/) were searched and reviewed to decipher whether any of the transformed genes were linked to allergenicity or human health.The EVALLER analysis identified 5 allergenic genes,whilst our literature review found 11 genes that were either related to allergic cases or to clinical adverse events;all 16 of these genes have been used in GMOs.The analysis pathway that we have developed can help guide the selection of genes to be used in genetic modification.The pathway also provides a paradigm for allergenicity analysis of transgene candidates.     

Preparation of composite tubular grafts for vascular repair via electrospinning

A novel type of composite vascular graft was developed via electrospinning in the present investigation.Collagen and chitosan were blended to form the inner and outer layer.Poly(1-lactide-co-caprolacto...     

Electroch emical properties and interfacial reactions of LiNi0.5Mn1.5O4-δ nanorods

LiNi0.5Mn1.5O4-δ which possesses a high voltage of 4.7 V vs.Li+/Li and stable structure has been considered as a promising cathode material for high energy Li-ion batteries.In this study,well-crystalli...     

Reduction behavior and kinetics of vanadium-titanium sinters under high potential oxygen enriched pulverized coal injection

In this work, the reduction behavior of vanadium-titanium sinters was studied under five different sets of conditions of pulverized coal injection with oxygen enrichment. The modified random pore model was established to analyze the reduction kinetics. The results show that the reduction rate of sinters was accelerated by an increase of CO and H₂ contents. Meanwhile, with the increase in CO and H₂ contents, the increasing range of the medium reduction index (MRE) of sinters decreased. The increasing oxygen enrichment ratio played a diminishing role in improving the reduction behavior of the sinters. The reducing process kinetic parameters were solved using the modified random role model. The results indicated that, with increasing oxygen enrichment, the contents of CO and H₂ in the reducing gas increased. The reduction activation energy of the sinters decreased to between 20.4 and 23.2 kJ/mol.     

BotGuard: Lightweight real-time botnet detection in software defined networks

The distributed detection of botnets may induce heavy computation and communication costs to network devices. Each device in related scheme only has a regional view of Internet, so it is hard to detect botnet comprehensively. In this paper, we propose a lightweight real-time botnet detection framework called Bot-Guard, which uses the global landscape and flexible configurability of software defined network (SDN) to identify botnets promptly. SDN, as a new network framework, can make centralized control in botnet detection, but there are still some challenges in such detections. We give a convex lens imaging graph (CLI-graph) to depict the topology characteristics of botnet, which allows SDN controller to locate attacks separately and mitigate the burden of network devices. The theoretical and experimental results prove that our scheme is capable of timely botnet detecting in SDNs with the accuracy higher than 90% and the delay less than 56 ms.     

Microstructure evolution and mechanical properties of a large-sized ingot of Mg-9Gd-3Y-1.5Zn-0.5Zr (wt%) alloy after a lower-temperature homogenization treatment

In this paper, a large-sized ingot of Mg-9Gd-3Y-1.5Zn-0.5Zr (wt%) alloy with a diameter of 600 mm was successfully prepared by the semi-continuous casting method. The alloy was subsequently annealed at a relatively low temperature of 430℃ for 12 h as a homogenization treatment. The microstructure and room-temperature mechanical properties of the alloy were investigated systematically. The results show that the as-cast alloy contained a mass of discontinuous lamellar-shaped 18R long-period stacking ordered (LPSO) phases with a composition of Mg₁₀ZnY and an α-Mg matrix, along with net-shaped Mg₅(Y,Gd) eutectic compounds at the grain boundaries. Most of the eutectic compounds dissolved after the homogenization treatment. Moreover, the amount and dimensions of the lamellar-shaped LPSO phase obviously increased after the homogenization treatment. The structure of the phase transformed into 14H-type LPSO with composition Mg₁₂Zn(Y,Gd). The mechanical properties of the heat-treated large-sized alloy ingot are uniform. The ultimate tensile strength (UTS) and tensile yield strength (TYS) of the alloy reached 207.2 MPa and 134.8 MPa, respectively, and the elongation was 3.4%. The high performances of the large-sized alloy ingot after the homogenization treatment is attributed to the strengthening of the α-Mg solid solution and to the plentiful LPSO phase distributed over the α-Mg matrix.     

Identification of protein superfamily from structurebased sequence motif

The structure-based sequence motif of the distant proteins in evolution, protein tyrosine phosphatases (PTP) Ⅰ and Ⅱ superfamilies, as an example, has been defined by the structural comparison, structure-based sequence alignment and analyses on substitution patterns of residues in common sequence conserved regions. And the phosphatases Ⅰ and Ⅱ can be correctly identified together by the structure-based PTP sequence motif from SWISS-PROT and TrEBML databases. The results show that the correct rates of identification are over 98%. This is the first time to identify PTP Ⅰ and Ⅱ together by this motif.     

Occupant evacuation model based on cellular automata in fire

By applying the rules set in traffic flow and pedestrian flow models, a basic cellular automata model is presented to simulate occupant evacuation in fire. Some extended models are introduced to study the special phenomena of evacuation from the fire room. The key of the models is the introduction of the danger grade which makes the route choice convenient and reasonable. Fire not only influences the emotional and behavioral characteristics of an individual but also affects his physical constitution, which reduces his maximal possible velocity. The models consider these influence factors by applying a set of simple but effective rules. It is needed to emphasize that all rules are established according to the essential phenomenon in fire evacuation, that is, all the occupants would try to move to the safest place as fast as possible. Some simulation examples are also presented to validate the applicability of the models.     

逆变器预置开关角误差对输出谐波的影响

针对数字控制技术的特点，分析了在选择性谐波消除法的理论计算结果与实际开关角数字控制结果出现偏差时，对逆变器输出电压谐波含量的影响。并研究了开关角数量对逆变器性能的影响，结果表明：开关角控制误差对逆变器输出谐波有一定影响，但可以在计算时修正；而开关角数量的增加有利于减小电流脉动，提高输出电压质量，但开关频率随之增加。     

一种混联式混合动力驱动系统的性能分析

对一种混联式混合动力驱动系统建立了相应的分析模型，并结合6600型中巴车对该驱动系统和整车的性能进行了理论分析；简要论述了该系统的工作机制和控制策略。与内燃机及纯电动汽车的性能进行了对比，为混合动力系统的实际应用提供了理论依据。