微生物基因芯片鉴定及再生利用

生物芯片是一种重要的生物分子检测方法,在科研和医学应用领域被广泛关注。该文以大肠杆菌和黄单胞菌检测为例研究微生物的基因芯片鉴定方法和生物芯片的再生使用价值,采用一种自主研制的新型微阵列芯片扫描仪对微生物鉴定和生物芯片的重复利用开展理论与实验研究。结果表明基因芯片鉴定微生物是非常可靠的,所设计的生物分子探针与芯片表面修饰分子的结合很牢固,5次重复杂交清洗后依然可以产生良好的荧光信号,从而验证了再生芯片使用的可行性。为了提高生物芯片的重复使用次数,论文还对生物芯片的设计与荧光标记方法进行了分析讨论。     

浅析低压供电设计计算

根据低压供电系统的整定计算原则,分析了低压供电设计计算中遇到的问题,提出了利用新设备和新技术的优势,解决供电设计中的难题。     

Meteorological observations on Mount Everest in 2005

Mount Everest,the highest point on the Earth is often referred to as the earth's third pole as such the place is relatively inaccessible and little is known about its meteorology.In April 2005,an automatic weather station was installed at the mountain's North Col(6523 m a.s.l.).According to the observational 10-minute mean and daily records,the meteorological characteristics were analyzed. All the meteorological elements displayed obvious diurnal variations during May 1 to July 22,2005.The monthly variation of daily meteo- rological elements on Mount Everest coincided with that on Dingri,the closest routine meteorological station,with the high correlation co- efficients of 0.928,0.877,0.682,0.755,0.826 and 0.676(n=83,p<0.001)for mean temperature,minimum temperature,max- imum temperature,relative humidity,pressure and wind speed,respectively.Furthermore,the vertical mean gradient of temperature was above 0.6℃/100 m,especially for the daily maximum temperature.Most weather events on Mount Everest prominently appeared on the same day as those on Dingri,especially those from daily mean pressure,temperature and relative humidity with the cross-correlation coeffi- cients of 0.673,0.485 and 0.487(n=83,p<0.001),respectively.Some other weather events on Mount Everest lagged one-day be- hind those on Dingri.Furthermore,forecasting of the weather events on Mount Everest from pressure on Dingri was more reliable than those from the other meteorological elements.The conclusions are much important for research on meteorology and climate changes in the region.     

Numerical simulation on the evolution of sediment waves caused by turbidity currents

Interest in the forming mechanism of sediment waves increases recently because of its significance on submarine engineering, sedimentary dynamics and hydrocarbon reservoir prediction in deep water. In this paper, the time-averaged continuity equations and Reynolds-averaged Navier-Stokes equations are applied in the numerical simulation of fluid dynamics. The modeling results are used to illuminate the effects of topography on turbidity current and explore the origin of submarine sediment waves. The research results show that (1) deposition occurs firstly at the lower ramp due to the deceleration of fluid, increase of density, loss of flow capacity and longer duration of flow passage; (2) density increase at the upslope due to the local jam results in velocity decrease and pressure increase; (3) sediment waves begin to be formed and migrated toward upstream in an area far away from the source with in- crease of the turbidity events; (4) deposition becomes more slowly with decrease of grain sizes, but the shape and sequences of these deposits are controlled by topography, not grain size.     

生物材料应用于脊髓损伤修复的研究进展

脊髓损伤是人类致残率最高的疾患之一。治疗脊髓损伤的趋势是联合应用多种技术和手段,创造出最适宜神经细胞再生恢复的环境,达到功能恢复的目的。生物材料在结构和功能上的优良性质使得它在脊髓损伤和修复中具有很好的应用前景。近年来兴起的组织工程学方法更为脊髓损伤的治疗带来新思路,即通过种子细胞—支架材料—神经营养因子复合物修复受损脊髓。本文回顾了近年来组织工程支架和细胞载体系统在脊髓损伤修复中的应用,展示了生物材料在组织工程和细胞分子治疗策略中的优越性。     

珠江三角洲网河潮汐空间特征

珠江三角洲网河区径潮作用复杂，其潮汐的特征明显受到径流、地形等影响。本文根据1999年洪季和2001年枯季的实测逐时潮位资料，应用数理统计、作图、潮汐调和分析等方法来分析珠江三角洲网河的潮汐空间特征，包括洪、枯季平均潮位的空间变化，潮汐调和常数（分潮振幅和迟角）的空间分布特征及沿程变化。同时，这些潮汐特征也反映了潮波在传播过程中受到径流、地形和边界条件作用后能量和周期的变化。     

The mechanism of HIT/DLR prosthetic hand

A multi-sensory five-fingered prosthetic hand has been designed on the basis of underactuated mechanism and coupling principle. The hand is much similar to the adult hand. Three motors actuate the thumb, the index finger and the other three fingers each. The hand comprises 13 joints. Actuated by a motor, the thumb can move along a conic surface, which is superior in appearance. Driven by another motor with springs as transmission elements, the mid finger, ring finger and little finger can envelop objects with complex shape. The sensory system and the appearance design are introduced. It was verified by experiments that the hand has strong capacity of self adaptation and can accomplish accurate and powerful grasp for objects with complex shape.     

Study on seal improvement and rotor thrust control of centrifugal compressor

Fluid pressure variations due to process fluctuations or balance drum seal degradation can result in rotor thrust increasing that may jeopardize thrust bearing and compressor’s reliability. Also, the leakage flow through balance drum seal can seriously affect the efficiency of compressor. A method that can improve both the efficiency and reliability of centrifugal compressor is presented. The method focused on rotor thrust control and balance drum seal upgrading. The low leakage feature of Dry-Gas-Seal（DGS）, high reliability of labyrinth, and the feasibility of upgrading existing structure are taken into account at the same time to design a combined labyrinth-dry gas seal system on the balancing drum. Based on the combined seal system, a Fault Self-Recovering（FSR） system for the fault of rotor shaft displacement is introduced to assure the safety and reliability of centrifugal compressor. The modern Computational Fluid Dynamics（CFD） is used to validate this envision. The numerical result and relevant information indicate that the combined sealing system could improve the efficiency of the centrifugal compressor by about 4%.     

锆钛酸铅压电陶瓷材料凝胶注模成型

为制备凝胶注模成型所需的高固相低粘度的锆钛酸铅陶瓷浆料,分别用分散剂聚甲基丙烯酸铵和柠檬酸三铵进行试验.研究了分散剂聚甲基丙烯酸铵、浆料pH值和固相含量对浆料粘度的影响,实验结果表明该分散剂最佳用量为陶瓷粉体质量分数0.30%~0.45%、浆料最佳pH值8.5~11.0,此时可制备体积分数50%、粘度小于1 Pa.s,适于凝胶注模的稳定锆钛酸铅浆料.其中当分散剂质量分数为0.30%时,烧结陶瓷有较好的微观结构.同时,对干压成型中不同粘合剂的作用效果进行了比较.