首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   58171篇
  免费   1686篇
  国内免费   2491篇
系统科学   2174篇
丛书文集   2067篇
教育与普及   2233篇
理论与方法论   255篇
现状及发展   291篇
研究方法   207篇
综合类   55109篇
自然研究   12篇
  2024年   342篇
  2023年   766篇
  2022年   986篇
  2021年   1186篇
  2020年   723篇
  2019年   292篇
  2018年   469篇
  2017年   649篇
  2016年   598篇
  2015年   1288篇
  2014年   1803篇
  2013年   1635篇
  2012年   1877篇
  2011年   2189篇
  2010年   2066篇
  2009年   2468篇
  2008年   2841篇
  2007年   2756篇
  2006年   2098篇
  2005年   2195篇
  2004年   1503篇
  2003年   1261篇
  2002年   1289篇
  2001年   1492篇
  2000年   1690篇
  1999年   3068篇
  1998年   2872篇
  1997年   3021篇
  1996年   2742篇
  1995年   2374篇
  1994年   2243篇
  1993年   1939篇
  1992年   1679篇
  1991年   1434篇
  1990年   1288篇
  1989年   1147篇
  1988年   918篇
  1987年   609篇
  1986年   344篇
  1985年   113篇
  1984年   30篇
  1983年   8篇
  1982年   7篇
  1979年   5篇
  1974年   6篇
  1973年   4篇
  1970年   4篇
  1968年   4篇
  1966年   3篇
  1957年   3篇
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
791.
Since the Quaternary scientists successfully dis-closed the paleoclimatic significance of the loess-paleo- sol sequence in the Loess Plateau, Northwest China, the loess-paleosol sequence has become well-known terres-trial materials revealing the multiple cyclic changes of Quaternary climates. As a carrier of paleoenvironmental information, it could compare beauty with the deep ocean deposit[8]. In recent years, however, the researchers began to put their sights to southern China. They try t…  相似文献   
792.
The L protein (241kD) of vesicular stomatitis virus (VSV) is the most important snbnnit of the replication complex. The existence of specific localization signal in the L protein was investigated by making recombinant constructs expressing truncated mutants of the L protein fused to green fluorescent protein (GFP) in transient transfection assays. The chimeric genes encoding varied N-terminal of L and GFP gene were put under the control of T7 promoter or CMV promoter. The fusion proteins were transiently expressed in BHK-21, COS-7, CHO or Hep G2 cells. When more than 120 residues were deleted or only 96 residues were kept on the N-terminal, the fusion proteins were shown to be distributed throughout the cells, cytoplasm and nucleus under the confocal microscope. However, other chimeric proteins with 120 or more amino acids were dotted and distributed in the perinuclear regions. And the fusion protein with 96—120 aa has the similar distribution. A thirteen-residue peptide QGYSFLHEVDKEA (108—120) was identified as localization signal, whose function would be absolutely distributed with the deficiency of D or V. Our results show that there is an independent localizing signal in N-terminal domain of L protein of VSV and this functional signal is conserved in different cell lines.  相似文献   
793.
The Hexi Corridor is located at the transition zone of the Asian summer monsoon and westerly airflow, and lies in an important position in terms of its ecological fragility and climatic sensitivity. During a recent field expedition to this region, we found a sedimentary mirabilite layer in a number of localities including Suwushan, Yanchi, Baitujing, Yanchi Gaotai, Huahai, Yumen, Halanuo扙r, and Dunhuang (Fig. 1), which have the potential to provide important information about environmenta…  相似文献   
794.
With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.  相似文献   
795.
Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.  相似文献   
796.
Cold injury is an important limitation of rice production. Therefore, screening for cold-tolerant genetic resources and the development of highly cold-tolerant cultivars is crucial for higher yield potential and stable yield of rice. There have been several reports on the genetic analysis and QTL mapping of the cold-tolerance at the booting stage. Toriyama et al.[1,2] and Futsuhara et al.[3,4] reported that 4 or more genes were involved in the cold-tolerance. Several QTLs at the booting sta…  相似文献   
797.
798.
As the third summary report of ODP Leg 184 to the South China Sea (SCS), this paper discusses the evolution of the East Asian monsoon and the SCS basin. A multi-proxy approach, involving geochemistry, micropale-ontology, pollen and other analyses, was adopted for reconstructing the evolutionary history of the East Asian monsoon, which was characterized by a series of paleo-climate events especially at 8, 3.2, 2.2 and 0.4 Ma. The new record indicates similar stages in the development of the East and South Asian monsoons, with an enhanced winter monsoon over East Asia being the major difference. The rich spectrums of monsoon variability from the southern SCS also reveal other characteristic features of the low latitude ocean. Evidence for the evolution of the SCS includes the hemipelagic Oligocene sediments, implying the existence of deep water environments during the early seafloor spreading stage of the SCS basin. The four major unconformities and some remarkabl ediagenetic features in upper Oligocene deposits indicate the strongest tectonic events in the region. From a careful comparison of lithologies and sedimentation rates, we conclude that the prominent differences in sedimentary environments between the southern and northern SCS were established only by ~3 Ma.  相似文献   
799.
南海海底特征、资源区位与疆界断续线   总被引:9,自引:0,他引:9  
根据南海海底的成因与地形特征 ,并通过建立和分析南海海底数字地形模型 ,将南海的海底分为中央的深海盆地、两侧的阶梯状大陆坡、以及周边亚洲大陆延伸的大陆架 3个地貌单元 .再据 194 7年“南海诸岛位置图”精确计算出二战后依据波茨坦公告为国际认可的断续线疆界的经纬度坐标 ,阐述了中国对南海主权的历史延续性 .最后 ,论述了南海区位与资源的优势 ,论证了其海域具体位置与巨大的经济政治战略意义 .  相似文献   
800.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号