Real time observation of the photocleavage of single DNA molecules

A method for real time observation of photo-cleavage of stratched λDNA at single molecular level by a fluorescent microscope coupled with CCD is developed.DNA molecules stained with YOYO-1 are stretched by the mo-lecular combing technique and fixed on a modified slide.Then the Process of Photocleavage and relaxation of DNA under radiation of blue light is observed.We speculate that the conformation change of stretched DNA and the effect of water are likely to facilitate the effect of YOYO photocleav-age DNA molecules.The photocleavage effect of YOYO for stretched DNA may be useful to study DNA elasticity,cancer research as well as the interaction between DNA and dyes.     

A new species of Gobiconodon （Triconodonta, Mammalia） and its implication for the age of Jehol Biota

A new species of Gobiconodon is found from the Yixian Formation of western Liaonlng, China. The new taxa,G zofiae sp. nov., has a confluent opening for branches Ⅱand Ⅲ of the trigeminal nerve on the anterior lamina of the petrosal. G zofiae sp. nov. is similar to Repenomamus in having an ossified Meckel‘s cartilage connecting the lower jaws and ear region. The new species, with enlarged I^1/I1, posteriorly located infraorbital foramen and four mental foramina,distinctly differs from the other species of Gobiconodon. The new material indicates that Gobiconodon has four, not five,upper molariforms. The presence of Gobiconodon in Jchol Biota makes it possible to correlate Jehol Biota with faunas in eastern Asia and North America, and suggests the age of the Yixian Formation to be Early Cretaceous.     

Do multiple cycles of aeolian deposit-pedogenesis exist in the reticulate red clay sections in southern China?

Since the Quaternary scientists successfully dis-closed the paleoclimatic significance of the loess-paleo- sol sequence in the Loess Plateau, Northwest China, the loess-paleosol sequence has become well-known terres-trial materials revealing the multiple cyclic changes of Quaternary climates. As a carrier of paleoenvironmental information, it could compare beauty with the deep ocean deposit[8]. In recent years, however, the researchers began to put their sights to southern China. They try t…     

新型吸附式冷管的最低制冷温度影响因素研究

提出了一种新型的余热固体吸附式制冷管．该种制冷管无节流装置、无运动部件、结构简单、强度较高，且每根管子自成一个完整独立的制冷系统，所以其密封性好、使用方便；研究了在吸附剂量不变的情况下，制冷剂(吸附质)充注量对制冷管性能的影响。由于该种制冷管的性能跟外部环境参数具有较强的耦合性，本文就热源温度、环境温度、风速和环境空气湿度等参数对其最低制冷温度的影响进行了实验研究，实验采用的工质对为13X沸石分子筛-水．     

钢筋混凝土轴拉构件刚度试验研究

通过理论分析与实验研究，提出了钢筋混凝土轴拉构件正常使用时的刚度计算公式，且分析了公式计算值与实测值的符合程度及其影响因素。     

壳聚糖在膜分离上的应用

膜分离是工业上能耗最低的一种分离过程，选择合适的膜材料和制膜工艺，一直是分离膜研究的核心．随着全球新材料的开发朝着环境友好材料方向的发展，壳聚糖作为一种无毒、无污染、可生物降解的天然高分子，受到了国内外学者的广泛关注．文章综述了壳聚糖在反渗透膜、超滤膜、渗透-蒸发膜和气体分离膜上的应用及其研究进展．     

四乙基铵碳酸氢盐水主客体加合物的晶体结构研究

合成了(C2H5)4N+HCO-3*H2O加合物并用X射线单晶衍射解析了其晶体结构.晶体属单斜晶系, P21/n空间群, a=0.8186(1) nm, b=1.5330(3) nm, c=0.9562(3)nm, β=97.82(2)°, Z=4, V=1.1888(5) nm3, Mr=209.28, ρc=1.169g*cm-3, μ(MοKα)=0.090mm-1, F(000)=464,对1837个独立衍射点的最终偏离因子R=0.0486.每个不对称单位由1个碳酸氢根离子、1个水分子与1个四乙基铵阳离子组成.阴离子和水分子之间通过氢键连成皱折纽带,被包含在四乙基铵阳离子列所形成的未完全封闭管道中.     

在Co2+注入修饰电极上米托蒽醌的电化学行为及其应用

米托蒽醌(MX)在0.005mol*L-1 Tris-0.05mol*L-1 NaCl (pH7.1)缓冲溶液中, 在Co离子注入玻碳修饰电极(Co/GCE)上,有一灵敏的伏安还原峰,峰电位为-0.68V,峰电流与MX的浓度在1.8×10-7～9.0×10-6 mol*L-1 间呈线性关系,检出限为9.0×10-8 mol*L-1.将该法用于病人尿样的测定,回收率为94.6%～105.7%. 用线性扫描和循环伏安法研究该体系的电化学行为和电催化.实验表明,体系为不可逆过程.Co/GCE上的电流比GC电极的大,扫速对催化效率的影响实验证明,存在着催化作用.X射线光电子能谱(XPS)和俄歇电子能谱(AES)实验表明,Co确实被注入在GC表面,注入的Co催化了MX的还原.     

两台三绕组变压器并列运行的差动保护

露天矿的采、运、排设备大都为电气设备，保证露天矿设备的安全供电，对露天矿的正常生产非常重要。随着负荷的变化，变电站的运行方式也在不断变化，主变压器的分列和并列运行非常频繁，作为变压器主保护的差动保护也应该及时做出调整。文章对两台三绕组变压器并列运行时的差动保护作了计算分折，以用于指导变电运行工作。