Real time observation of the photocleavage of single DNA molecules

A method for real time observation of photo-cleavage of stratched λDNA at single molecular level by a fluorescent microscope coupled with CCD is developed.DNA molecules stained with YOYO-1 are stretched by the mo-lecular combing technique and fixed on a modified slide.Then the Process of Photocleavage and relaxation of DNA under radiation of blue light is observed.We speculate that the conformation change of stretched DNA and the effect of water are likely to facilitate the effect of YOYO photocleav-age DNA molecules.The photocleavage effect of YOYO for stretched DNA may be useful to study DNA elasticity,cancer research as well as the interaction between DNA and dyes.     

连续变量问题全局优化的模拟退火法

本文针对过程系统连续变量优化问题中普遍存在的多峰现象, 探索了应用模拟退火法求解其全局最优解。文中根据连续变量问题的特性, 提出了一种相邻状态的产生函数和迭代方案, 并分析了模拟退火过程的起始温度、终止温度以及降温速度等参数对优化计算的影响, 给出了这些参数的适宜区域, 通过三个例题的计算, 将模拟退火法与传统优化方法一梯度法进行了对比分析, 结果表明该法能够有效地解决传统的确定型优化方法所不能奏效的全局优化问题。     

海洋石油平台管件加工的精确几何建模

结合目前国产数控相贯线切割机普遍配备双倾角构型割炬的技术特点,对传统管材切割工艺进行了改进.通过对传统切割工艺中的几何近似部分进行精确几何建模,获得精确几何解,并通过双旋转自由度构型的割炬,完成基于该精确几何解的切割角精确切割.仿真实验表明:相对于传统的近似切割工艺,该切割角精确切割工艺可有效减少管件端面的有效切割长度,提高了切割速度和时间效率,且切割出的管材端面更符合曲面的几何性质,有利于焊接面之间的曲面匹配度.     

太阳能制冷降温技术在我区开发利用的前景

指出利用太阳能制冷降温的优越性，介绍各种太阳能制冷方案．分析广西的气候特点．介绍引进ＯＭ太阳房技术，设计符合本地条件的太阳能去湿降温建筑．     

高速公路拼接技术及施工要点

道路拼接技术可以充分利用原路面结构,具有较好的经济性,在高速公路改扩建工程中得到广泛应用。本文从高速公路拼接方式和材料应用角度出发,重点对拼接技术中最为重要的层间粘结施工环节进行分析,并对基层、面层拼接的施工程序和技术要点进行了阐述。     

实时定量PCR检测B细胞恶性肿瘤中BCL11A基因的表达水平

目的:建立B细胞淋巴瘤/白血病BCL11A基因的定量检测方法,并分析其在B细胞恶性肿瘤中的表达水平。方法:利用实时定量RT-PCR分析B细胞淋巴瘤(18例)、B细胞性慢性淋巴细胞白血病(B-CLL,8例)、T细胞性急性淋巴细胞白血病(T-ALL,8例)和正常对照(15例)外周血单个核细胞(PBMC)中BCL11A基因的表达水平,以甘油醛-3-磷酸脱氢酶(GAPDH)基因作为内对照。结果:B-CLL组和B细胞淋巴瘤组患者PBMC中BCL11A表达水平均明显高于正常对照(P=0.000)和T-ALL组(P=0.000);T-ALL组和正常对照组BCL11A表达水平无显著差别(P=0.084);B-CLL组和B细胞淋巴瘤组BCL11A表达水平无显著差别(P=0.776)。在B细胞淋巴瘤不同的病例中,BCL11A表达水平有较大差异,其中最小值为0.04,最大值为9.70,中位值为1.00。结论:成功建立BCL11A基因的定量检测方法。     

贵州省地萼苔科植物初步研究

根据文献记载以及对采自贵州省各县市地萼苔科Geocalycaceae植物标本(300余份)的鉴定,经统计贵州省地萼苔科植物现有18种l变种,其中中华裂萼苔Chiloscyphus sinensis、爽气裂萼苔C.fragrans为贵州新记录.根据各个种的形态特征,编制了该科的分种检索表,并对新记录种的主要形态特征进行了...     

信号交叉口人机混驾交通流速度控制策略建模

为分析人机混驾交通流下网联自动驾驶车辆（connected and autonomous vehicles,CAV）速度控制策略对交通流运行特征的影响,构建了考虑驾驶员对行车信息获取不确定性的人工驾驶车辆交叉口通行决策模型。提出考虑前车速度影响的自动驾驶速度控制策略,构建信号交叉口连续型元胞自动机更新规则,通过引入不同CAV渗透率、道路饱和度、控制区长度参数,研究CAV速度控制策略对信号交叉口交通流运行特征的影响。结果表明：CAV能显著提高交叉口通行能力,且车流通过交叉口区域的延误显著降低;同时速度控制策略的实施效果还受控制区长度的影响,呈现出随着控制区长度的增加,车均延误逐渐降低并趋于稳定。     

Meta-analysis of genome-wide association data and large-scale replication identifies additional susceptibility loci for type 2 diabetes

Genome-wide association (GWA) studies have identified multiple loci at which common variants modestly but reproducibly influence risk of type 2 diabetes (T2D). Established associations to common and rare variants explain only a small proportion of the heritability of T2D. As previously published analyses had limited power to identify variants with modest effects, we carried out meta-analysis of three T2D GWA scans comprising 10,128 individuals of European descent and approximately 2.2 million SNPs (directly genotyped and imputed), followed by replication testing in an independent sample with an effective sample size of up to 53,975. We detected at least six previously unknown loci with robust evidence for association, including the JAZF1 (P = 5.0 x 10(-14)), CDC123-CAMK1D (P = 1.2 x 10(-10)), TSPAN8-LGR5 (P = 1.1 x 10(-9)), THADA (P = 1.1 x 10(-9)), ADAMTS9 (P = 1.2 x 10(-8)) and NOTCH2 (P = 4.1 x 10(-8)) gene regions. Our results illustrate the value of large discovery and follow-up samples for gaining further insights into the inherited basis of T2D.