A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

The objective of this study is to propose a more accurate and faster MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r ²=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125~32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7~8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24~28 h for ADA. The MCA provides an accurate and rapid method for quantification of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.     

The Symbolic Meanings of the Forest in The Scarlet Letter by Nathaniel Hawthorn

The symbols in Hawthorne’s masterpiece The Scarlet Letter have the characteristics of indefiniteness and multi-sense. Each character brings out a different side of the forest,however the forest also brings out a different side in each character.The forest reflects multi-dimension. The image of ＂woods＂ or ＂forest＂ in The Scarlet Letter manifests Horthorne＇s understanding and exploring of the human nature. The forest may stand for individual freedom,innocence,truth,danger and evil as well.     

关于改进高职学生数学学习困难的探讨和研究

从学生的认知角度出发,对高职学生数学学习困难的认知特点及元认知特点进行了探讨,并提出了改进的方法和建议。     

CONSTRUCTIVE KISSING NUMBERS IN HIGH-DIMENSIONAL SPACES

In the present paper, we focus on constructive spherical codes. By employing algebraic geometry codes, we give an explicit construction of spherical code sequences. By making use of the idea involved in the proof of the Gilbert-Varshamov bound in coding theory, we construct a spherical code sequence in exponential time which meets the best-known asymptotic bound by Shamsiev and Wyner.     

中英师范教育专业课程对比分析及思考

通过对中英师范教育专业课程设置的对比分析，肯定英国师范课程设置的优势；指出我国师范课程设置的不足．阐述我国师范课程改革的必要性以及重构我国师范课程的个人观点。     

信号与系统中的matlab程序

在现行的理工科教学中,matlab的应用已经成为一个重要的内容。尤其在电子类的核心专业课信号与系统中,matlab的作用更加值得教师关注。文章就信号与系统这一课程中牵涉到的一些典型matlab程序,进行了注解和说明。试图在信号与系统和matlab的应用两方面找到了一个更为恰当的结合点,使学生和教师在专业知识和应用性工具方面,得到一个更好的处理方式。     

论建立学校安全责任强制保险制度

学校安全事故频繁发生,我国现在学校安全保险法律制度不完善。作者分析了中国目前学校安全责任制度存在的问题,并提出了关于建立学校安全责任绝对强制保险制度的建议。     

基于循环经济模式的交通运输可持续发展研究

文章阐明了循环经济的基本概念和基本理论，在分析我国交通运输业可持续发展面临的诸多问题后，结合循环经济模式对我国交通运输可持续发展战略进行了研究。     

Multiple MIPS 4Kc cores based interrupt controller design and its implementation on HDTV SoC platform

A multiple MIPS 4Kc processor cores based interrupt processing system is introduced. The interrupt controller plays a key role in the high definition television （HDTV） system-on-a-chip （SoC） platform, especially when it is a multiple processor system. Based on a general introduction to the whole HDTV SoC platform, a layered interrupt controller and its implementation are discussed in detail. The proposed scheme was implemented in our FPGA verification board. The results indicate that our scheme is reliable and efficient. Meanwhile, as a functional intellectual property （IP）, the interrupt controller has reusability and expandability with the layered structure.     

普通高校体育教育专业田径专修跨栏教学素质教育模式的再探

对体育教育专业如何与“素质教育”良性接轨进行了探索与研究,旨在能够为全面推进体育教育专业素质教育改革起到抛砖引玉的作用.研究结果表明：设计的教学模式有助于实现从应试教育向素质教育的良性转化;还有助于在普通高校体育教育专业田径专修跨栏教学中增强学生的自信心,提高教与学的效率.