Proteome changes in the plasma of Papilio xuthus (Lepidoptera: Papilionidae): effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera: Pteromalidae)

Although the biochemical dissection of parasitoid-host interactions is becoming well characterized, the molecular knowledge concerning them is minimal. In order to understand the molecular bases of the host immune response to parasitoid attack, we explored the response of Papilio xuthus parasitized by the endoparasitic wasp Pteromalus puparum using proteomic approach. By examining the differential expression of plasma proteins in the parasitized and unparasitized host pupae by two-dimensional (2D) electrophoresis, 16 proteins were found to vary in relation to parasitization compared with unparasitized control samples. All of them were submitted to identification by mass spectrometry coupled with a database search. The modulated proteins were found to fall into the following functional groups: humoral or cellular immunity, detoxification, energy metabolism, and others. This study contributes insights into the molecular mechanism of the relationships between parasitoids and their host insects.     

蜂蜜中多种有机磷农药残留的液相色谱串联质谱检测

针对敌百虫、敌敌畏和蝇毒磷为常用的防治蜜蜂天敌或虫害的有机磷农药杀虫剂,易在蜂蜜中产生残留,建立了蜂蜜中敌百虫、敌敌畏和蝇毒磷残留的液相色谱串联质谱(LC-MS/MS)同时测定方法。蜂蜜样品经乙酸乙酯提取、浓缩,用甲醇溶解残渣,液相色谱(LC)分离,电喷雾(ESI)-MS/MS多反应监测(MRM)模式检测,外标基体曲线定量。3种有机磷农药在20～500μg/L范围内线性响应良好,相关系数均大于0.994;获得的检出限均为0.1μg/kg,定量限均为4μg/kg。在加标浓度为10～40μg/kg范围内,平均回收率为78.5%～96.8%,相对标准偏差为9.1%～13.1%。     

基于金属磁记忆的钢结构焊缝疲劳试验

金属磁记忆检测技术已经在对接焊缝疲劳损伤检测方面有了诸多研究成果,但是目前的研究主要集中于对接焊缝的拉压疲劳,与实际结构中焊缝的弯拉疲劳过程之间存在差异.为此,本研究开展了含对接焊缝的工字钢梁疲劳试验,旨在真实地模拟实际工程中对接焊缝的疲劳开裂过程,揭示疲劳过程中磁信号的变化规律.试验结果表明:焊缝的初始焊接状态对于磁信号分布曲线的变化趋势有一定的影响,对于同一焊缝,当其初始磁信号曲线分布趋势相似时,其峰值及谷值随疲劳周次的变化趋势基本一致;研究发现,磁信号分布曲线的突变可以表征宏观疲劳裂纹出现,但是在疲劳稳定阶段,磁信号法向分量对于疲劳损伤的累计更加敏感;经过标准化处理后的矢量合成磁场平均值特征曲线可以表征焊缝疲劳的变化过程,捕捉到疲劳裂纹萌生的广义时间节点,从而对宏观疲劳裂纹的出现起到预警作用.     

Fabrication of PEDOT/PSS-ZnO nanowire by self-assembly method under vacuum condition

Poly(3,4-ethylenedioxythiophene) (PEDOT) has at- tracted much attention due to its high conductivity, excel- lent environmental stability and chemical processabil- ity[1,2]. PEDOT/PSS dispersion is a water-soluble system using poly(styrene sulfonic acid) (PSS) as the doping counterion during polymerization. The inclusion of PSS results in better solubility and processability to the PE- DOT/PSS aqueous dispersion[3, 4]. Recently, synthesis of one-dimensional (1D) multifunc- tional co…     

一种无线通信信号超窄带滤波方法的改进

在无线通信过程中,采用传统超窄带滤波方法很难获取较宽且没有被干扰的频带,有效信号在频域中会出现裂口,导致大量数据丢失,造成人为干扰。提出一种新的无线通信信号超窄带滤波方法,依据幅值、斜率以及带宽对干扰源位置与数量进行判定,为无线通信信号超窄带滤波提供可靠依据。将干扰数据与有效数据投影至不同空间,分别对其进行超窄带滤波处理,通过数据特征值子空间滤波法,获取无线通信信号超窄带滤波的回波数据,实现无线通信信号超窄带滤波,完成无线通信信号超窄带滤波方法的改进。实验结果表明,所提方法能够有效滤除无线通信信号中的超窄带干扰,能保留数据的完整性,避免人为干扰。     

区域性发电机组检修计划优化与信息管理

提出了区域性发电机组检修计划的多目标规划模型,可对一个或多个目标函数随时进行检修计划的优化调整并评价其可靠性和经济性,既可保证电力系统的可靠性,又能减少电力系统的发电及检修费用,在此基础上开发了区域性发电机组优化检修计划编制与检修信息管理系统．实际应用表明,系统的优化计算模型是正确有效的．     

剥层测量残余应力的修正

已加工表面残余应力以地机械零部件的功能行为有很重要的影响。然而，到目前为止，已加工表面层内残余应力的测量仍存在许多问题。本文提出了表面剥层时残余应力测量（用X射线法或剥层线性方程法）的修正方法及修正公式。通过对试验数据修正计算，结果表明，应用此方法可大大提高测量精度，本文为残余应力测量修正提供了理论依据。作者还讨论了已加工表面残余应力测量修正的必要性问题。     

铁路主要干线提速有关问题的研究

在对列车提速的必要性、原则和有限目标进行论述的基础上，就列车提速对运输技术设备的要求和运输组织方面的若干问题进行了初步探讨，分析了多速度客货列车混跑时，不同速度配比的组合方案对线路通过能力的影响，为干线提速的方案选定提供了基础     

基于Prophet-DeepAR模型的Web流量预测

Web流量预测一直是数据中心网络的热点问题,对于提高网络服务质量具有重要意义。由于Web流量具有非线性、自相关性和周期性等复杂特点,对其准确预测有很大的挑战性。为充分挖掘出Web流量的可预测信息,同时使预测模型具有充分的可解释性和可配置性,本文提出一种基于Prophet和深度自回归(DeepAR)的组合预测模型。其中,Prophet是基于时序分解的加性模型,对Web流量的趋势、季节性周期、节假日信息进行建模。同时,使用基于概率预测的DeepAR模型对Prophet残差隐含的自回归信息建模,捕获长短期依赖关系,以减低Prophet残差的方差,并充分捕获Web流量的自回归信息。在真实的Web流量数据集上进行验证实验,结果表明在RMSE和MAE两项评价指标上均优于对比模型,验证了该组合模型的有效性。     

Seasonal variations and environmental controls on stalagmite calcite crystal growth in Heshang Cave,central China

Stalagmite growth rates are usually considered to reflect changes in paleoclimate and paleoenvironment. However, how exactly growth rates are affected by climate and environment is still unclear. We launched a monitoring and modeling program that lasted approximately 4 years on two active drip sites in Heshang Cave, central China. We collected comprehensive quantitative data on growth rates, cave temperature, CO₂ concentration, drip rate and drip-water chemistry to better understand the relationship between stalagmite growth and cave environment. By laying out glass substrates, we successfully grew stalagmite calcite crystals with rhombic characteristics, and quantified growth rates by measuring the long and short axes of calcite crystals under a microscope. Combined with coeval environmental data, we explicitly examined the roles of cave temperature, drip rate and drip-water chemistry in controlling the micro-scale growth of stalagmite calcite. Results show that growth of stalagmite calcite crystals at two drip sites exhibited similar seasonal variations − generally faster in the summer months, at ∼3 μm d⁻¹, and slower during the late winter to early spring, at ∼1.5 μm d⁻¹. Variations in calcite growth rates were mainly determined by changes in cave temperature, with the growth rate increasing by 8.1%/°C and 6.6%/°C at the two locations. This indicates the potential use of stalagmite growth rates as a seasonal-resolution paleo-temperature proxy in some ventilated caves. On the other hand, the effect of drip rate and drip-water Ca²⁺ and SIC values on growth rates were not significant.