Integration of telomere sequences with the draft human genome sequence

Telomeres are the ends of linear eukaryotic chromosomes. To ensure that no large stretches of uncharacterized DNA remain between the ends of the human working draft sequence and the ends of each chromosome, we would need to connect the sequences of the telomeres to the working draft sequence. But telomeres have an unusual DNA sequence composition and organization that makes them particularly difficult to isolate and analyse. Here we use specialized linear yeast artificial chromosome clones, each carrying a large telomere-terminal fragment of human DNA, to integrate most human telomeres with the working draft sequence. Subtelomeric sequence structure appears to vary widely, mainly as a result of large differences in subtelomeric repeat sequence abundance and organization at individual telomeres. Many subtelomeric regions appear to be gene-rich, matching both known and unknown expressed genes. This indicates that human subtelomeric regions are not simply buffers of nonfunctional 'junk DNA' next to the molecular telomere, but are instead functional parts of the expressed genome.     

分布式PowerBuilder应用程序的调试策略和方法

由于分布式应用的独特性,使得分布式应用程序的调试远比单机应用程序的调试复杂得多。文中对分布式应用程序提出一种“先单机单机程调试,次单机多进程调试,最后再多机远程调试”的调试策略,给出在PowerBuilder开发环境下的实施方法,并对分布式PB应用程序的常用调试工具、技术和调试中应注意的问题进行探讨。所述策略和方法知开发《广西变通高校计算机等级考试管理系统MCT》网络版中得到成功使用,证明是正确可行的。     

塔里木盆地前震旦-石炭纪构造与地层组合特征

通过对塔里木盆地前震旦-石炭纪构造演化与地层组合特征的分析研究,将该盆地构造和地层系统划分为2大组合系统5个构造-地层组合.前震旦系为混合片麻岩、浅变质岩组成的地台基底,震旦纪发生板内裂陷形成坳拉槽,早寒武世-奥陶纪和石炭纪是盆地两次大的海侵期,沉积了富含烃类的碳酸盐岩建造,志留-泥盆纪为克拉通边缘隆升阶段,发育浅海相碎屑岩及碳酸盐岩建造.     

一种小额支付协议的研究与设计

分析了PayWord这种小额支付协议,针对PayWord的一些关于交易原子性的局限性,提出了一种新的支付协议--CertWord,此协议沿用PayWord协议的Payword链来表示货币,充分利用交易证书对交易信息进行签名,并记录交易步骤的状态,从流程上保证交易的原子性。     

关于“14条”的几点议论

针对《谈高职院校生源短缺的原因与对策》中描述的 1 4条 ,列举了高职院校在办学规模、专业设置、教育教学改革等方面的成绩 ,从而阐述了与《对策》不同的看法     

Degradation of pyrene and characterization of<Emphasis Type="Italic">Saccharothrix</Emphasis> sp. PYX-6 from the oligotrophic Tianchi Lake in Xinjiang Uygur Autonomous Region,China

Bacterial strain PYX-6 that utilizes anthracene,phenanthrene, or pyrene for carbon and energy sources for growth was isolated from a non-polluted lake (Tianchi Lake) in Xinjiang Uygur Autonomous Region of China. Its morphology, physiological and biochemical properties, cell wall pattern and G C mol% content of DNA molecules were characterized. The 16S rRNA gene of strain PYX-6 was sequenced and analyzed for similarities to related bacterial species. Results indicated that strain PYX-6 is a member of the Genus Saccharothrix, and the strain was named Saccha-rothrix sp. PYX-6. When pyrene was the sole carbon source in cultural medium, the strain PYX-6 assimilated pyrene for growth and 0.005% of yeast extract stimulated pyrene degradation and assimilation. The optimal pH of cultural medium and the optimal shaking frequency during cultivation were 6-8 and 200 r/m, respectively. It was found that the disappearance of pyrene in medium occurred before significant growth of strain PYX-6 took place. Phthalic acid, benzylacetic acid, and benzylpropenoic acid were detected as catabolic intermediates during pyrene degradation with mass spectroscopy and this result indicated that Saccharothrix sp.PYX-6 adopted a pathway that is different from the pathway of the previously reported pyrene-degrading Mycobacterium sp. PYR-1.     

Effect of addition of poly（ethylene glycol） on electrical conductivity of poly（3,4-ethylenedioxythiophene）-poly（styrenesulfonate） hybrid

By mixing various concentrations of poly (ethylene glycol), a series of poly(3,4-ethylenedioxythiophene)-poly(styeenesuffonate) composite thin films were prepared.The electrical conductivity of the PEDOT-PSS/PEG thin films was measured by the four-pcobe method. Experimental results showed that the inclusion of poly(ethylene glycol) influenced the electrical conductivity of PEDOT-PSS film significantly. With the increase of PEG concentrations, the electrical conductivity sharply increased to reach a maximum and then slowly decreased down. Furthermore, the PEG molecular weight and environment temperature also played important roles on the electrical conductivity of PEDOT-PSS/PEG thin films. A good linear relationship was found between in σDC and T^-1/2 within the entire temperature range detected.     

Mutations of PVRL1, encoding a cell-cell adhesion molecule/herpesvirus receptor, in cleft lip/palate-ectodermal dysplasia

Cleft lip, with or without cleft palate (CL/P), is one of the most common birth defects, occurring in 0.4 to 2.0 per 1,000 infants born alive. Approximately 70% of CL/P cases are non-syndromic (MIM 119530), but CL/P also occurs in many single-gene syndromes, each affecting a protein critical for orofacial development. Here we describe positional cloning of the gene responsible for an autosomal recessive CL/P-ectodermal dysplasia (ED) syndrome (CLPED1; previously ED4; ref. 2), which we identify as PVRL1, encoding nectin-1, an immunoglobulin (Ig)-related transmembrane cell-cell adhesion molecule that is part of the NAP cell adhesion system. Nectin-1 is also the principal cell surface receptor for alpha-herpesviruses (HveC; ref. 7), and the high frequency of CLPED1 on Margarita Island in the Caribbean Sea might result from resistance of heterozygotes to infection by these viruses.     

Selection of peptides with semiconductor binding specificity for directed nanocrystal assembly

In biological systems, organic molecules exert a remarkable level of control over the nucleation and mineral phase of inorganic materials such as calcium carbonate and silica, and over the assembly of crystallites and other nanoscale building blocks into complex structures required for biological function. This ability to direct the assembly of nanoscale components into controlled and sophisticated structures has motivated intense efforts to develop assembly methods that mimic or exploit the recognition capabilities and interactions found in biological systems. Of particular value would be methods that could be applied to materials with interesting electronic or optical properties, but natural evolution has not selected for interactions between biomolecules and such materials. However, peptides with limited selectivity for binding to metal surfaces and metal oxide surfaces have been successfully selected. Here we extend this approach and show that combinatorial phage-display libraries can be used to evolve peptides that bind to a range of semiconductor surfaces with high specificity, depending on the crystallographic orientation and composition of the structurally similar materials we have used. As electronic devices contain structurally related materials in close proximity, such peptides may find use for the controlled placement and assembly of a variety of practically important materials, thus broadening the scope for 'bottom-up' fabrication approaches.     

浅谈排球立体进攻战术理论及实践方法

从现代排球立体进攻战术的演变与发展入手,阐述了立体进攻战术的主要特点,提出了培养与实施立体进攻战术的主要方法。